Recombinant Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19867] to TGF beta 1 + TGF beta 3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867]
See all TGF beta 1+TGF beta 3 primary antibodies -
Description
Rabbit monoclonal [EPR19867] to TGF beta 1 + TGF beta 3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A549, K562, HL-60, L-929, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal spleen, mouse spleen and rat spleen lysates; Recombinant protein fragment human TGF beta 1 and Recombinant protein fragment human TGF beta 3.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19867 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 44,12.5 kDa (predicted molecular weight: 44 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 44,12.5 kDa (predicted molecular weight: 44 kDa). |
Target
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Cellular localization
TGF beta 1: Secreted > extracellular space > extracellular matrix. TGF beta 3: Secreted. -
Database links
- Entrez Gene: 7040 Human
- Entrez Gene: 7043 Human
- Entrez Gene: 21803 Mouse
- Entrez Gene: 21809 Mouse
- Entrez Gene: 25717 Rat
- Entrez Gene: 59086 Rat
- Omim: 190180 Human
- Omim: 190230 Human
see all
Images
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All lanes : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/1000 dilution
Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 3 : HL-60 (Human promyelocytic leukemia cell line) whole cell lysate
Lane 4 : L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 44 kDa
Observed band size: 12.5,44 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1-3: 30 seconds; Lane 4: 3 minutes.
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Lane 1 : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/5000 dilution
Lanes 2-3 : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/1000 dilution
Lane 1 : Human fetal spleen tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 44 kDa
Observed band size: 12.5,44 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The cleaved form observed is consistent with what has been described in the literature (PMID:12717387; PMID:2139036; PMID:3208746).
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All lanes : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 44 kDa
Observed band size: 12.5,44 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
The cleaved form observed is consistent with what has been described in the literature (PMID:12717387; PMID:2139036; PMID:3208746).
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Lane 1 : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/10000 dilution
Lanes 2-3 : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/1000 dilution
Lane 1 : Recombinant protein fragment human TGF beta 1
Lane 2 : Recombinant protein fragment human TGF beta 2
Lane 3 : Recombinant protein fragment human TGF beta 3
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1/2: 3 minutes; Lane 3: 15 seconds.
Recombinant protein fragment human TGF beta 1 contains aa279-390 with a GST-tag. Recombinant protein fragment human TGF beta 2 contains aa303-414 with a GST-tag Recombinant protein fragment human TGF beta 3 contains aa301-412 with a GST-tag. These three fragment recombinant proteins were made in-house
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab205604 has been referenced in 1 publication.
- Harb I et al. Nicorandil prevents the nephrotoxic effect of cyclosporine-A in albino rats through modulation of HIF-1a/VEGF /eNOS signaling. Can J Physiol Pharmacol N/A:N/A (2020). PubMed: 32822562