Recombinant
RabMAb

Recombinant Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604)

Overview

  • Product name

    Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867]
    See all TGF beta 1+TGF beta 3 primary antibodies
  • Description

    Rabbit monoclonal [EPR19867] to TGF beta 1 + TGF beta 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human TGF beta 1 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P01137

  • Positive control

    • WB: A549, K562, HL-60, L-929, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal spleen, mouse spleen and rat spleen lysates; Recombinant protein fragment human TGF beta 1 and Recombinant protein fragment human TGF beta 3.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab205604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 44,12.5 kDa (predicted molecular weight: 44 kDa).

Target

Images

  • All lanes : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/1000 dilution

    Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
    Lane 3 : HL-60 (Human promyelocytic leukemia cell line) whole cell lysate
    Lane 4 : L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa
    Observed band size: 12.5,44 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1-3: 30 seconds; Lane 4: 3 minutes.

  • Lane 1 : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/5000 dilution
    Lanes 2-3 : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/1000 dilution

    Lane 1 : Human fetal spleen lysate
    Lane 2 : Rat spleen lysate
    Lane 3 : Mouse spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
    Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 44 kDa
    Observed band size: 12.5,44 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The cleaved form observed is consistent with what has been described in the literature (PMID:12717387; PMID:2139036; PMID:3208746).

  • All lanes : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa
    Observed band size: 12.5,44 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The cleaved form observed is consistent with what has been described in the literature (PMID:12717387; PMID:2139036; PMID:3208746).

  • Lane 1 : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/10000 dilution
    Lanes 2-3 : Anti-TGF beta 1 + TGF beta 3 antibody [EPR19867] (ab205604) at 1/1000 dilution

    Lane 1 : Recombinant protein fragment human TGF beta 1
    Lane 2 : Recombinant protein fragment human TGF beta 2
    Lane 3 : Recombinant protein fragment human TGF beta 3

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1/2: 3 minutes; Lane 3: 15 seconds.

    Recombinant protein fragment human TGF beta 1 contains aa279-390 with a GST-tag. Recombinant protein fragment human TGF beta 2 contains aa303-414 with a GST-tag Recombinant protein fragment human TGF beta 3 contains aa301-412 with a GST-tag. These three fragment recombinant proteins were made in-house

References

ab205604 has not yet been referenced specifically in any publications.

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