Overview

  • Product name
    Anti-TGF beta 1 antibody [2E6]
    See all TGF beta 1 primary antibodies
  • Description
    Mouse monoclonal [2E6] to TGF beta 1
  • Host species
    Mouse
  • Specificity
    Western blotting demonstrated that this antibody reacts with the dimeric (25 kDa) and monomeric (12.5 kDa.) forms of TGF-beta under both non-reducing and reducing conditions respectively. TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB. This antibody recognizes both human platelet-derived and recombinant TGF-beta in ELISA.
  • Tested applications
    Suitable for: Neutralising, WB, ELISAmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human TGF beta 1. TGF beta 1 from human platelets.

  • Positive control
    • WB: recombinant protein
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Neutralising Use at an assay dependent concentration. PubMed: 17574548
WB Use a concentration of 1 - 10 µg/ml.

Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.This antibody, used at this concentration will allow visualization of 100 ng/lane of TFG-beta.

ELISA Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
    • Tissue specificity
      Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
    • Involvement in disease
      Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
    • Sequence similarities
      Belongs to the TGF-beta family.
    • Post-translational
      modifications
      Glycosylated.
      The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cartilage-inducing factor antibody
      • CED antibody
      • Differentiation inhibiting factor antibody
      • DPD1 antibody
      • LAP antibody
      • Latency-associated peptide antibody
      • Prepro transforming growth factor beta 1 antibody
      • TGF beta 1 antibody
      • TGF beta antibody
      • TGF beta 1 protein antibody
      • TGF-beta 1 protein antibody
      • TGF-beta-1 antibody
      • TGF-beta-5 antibody
      • TGF-beta1 antibody
      • TGFB antibody
      • Tgfb-1 antibody
      • tgfb1 antibody
      • TGFB1_HUMAN antibody
      • TGFbeta antibody
      • TGFbeta1 antibody
      • Transforming Growth Factor b1 antibody
      • Transforming Growth Factor beta 1 antibody
      • Transforming growth factor beta 1a antibody
      • transforming growth factor beta-1 antibody
      • transforming growth factor, beta 1 antibody
      • Transforming Growth Factor-ß1 antibody
      see all

    References

    This product has been referenced in:
    • Rocks N  et al. ADAMTS-1 metalloproteinase promotes tumor development through the induction of a stromal reaction in vivo. Cancer Res 68:9541-50 (2008). WB ; Human . Read more (PubMed: 19010931) »
    • Schmid SA  et al. Lactate adversely affects the in vitro formation of endothelial cell tubular structures through the action of TGF-beta1. Exp Cell Res 313:2531-49 (2007). Read more (PubMed: 17574548) »
    See all 3 Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews

    Application
    Western blot
    Sample
    Guinea pig Tissue lysate - whole (Brain)
    Loading amount
    30 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Yvonne Sondy

    Verified customer

    Submitted May 21 2012

    Application
    Western blot
    Sample
    Human Cell lysate - other (Fibroblast)
    Loading amount
    30 µg
    Specification
    Fibroblast
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

    Abcam user community

    Verified customer

    Submitted Nov 21 2006

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