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    tgf-beta-1-antibody-ab9758.pdf

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Cardiovascular Angiogenesis Growth Factors TGF
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Anti-TGF beta 1 antibody (ab9758)

  • Datasheet
Reviews (2)Q&A (24)References (45)

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Western blot - Anti-TGF beta 1 antibody (ab9758)

    Key features and details

    • Rabbit polyclonal to TGF beta 1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-TGF beta 1 antibody
      See all TGF beta 1 primary antibodies
    • Description

      Rabbit polyclonal to TGF beta 1
    • Host species

      Rabbit
    • Specificity

      Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Sheep, Rabbit, Horse, Guinea pig, Hamster, Cow, PigDoes not react with: Dog
    • Immunogen

      Synthetic peptide corresponding to Human TGF beta 1.
      Sequence:

      FSSTEKN-C

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Growth Factors
      • TGF
      • Signal Transduction
      • Growth Factors/Hormones
      • TGF
      • Stem Cells
      • Signaling Pathways
      • TGF beta
      • Secreted
      • Cancer
      • Growth factors
      • TGF
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • Angiogenic growth factors
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Metabolism
      • Pathways and Processes
      • Cofactors, Vitamins / minerals
      • Co-factors
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human TGF beta 1 protein (Active) (ab50036)

    Applications

    Our Abpromise guarantee covers the use of ab9758 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/100 - 1/500.

    In reduced samples, the expected bands are 12 kDa (mature protein) and 44 kDa (unprocessed protein). Expected band at 25 kDa in non-reduced samples (homodimer of mature protein). Glycosylations may also cause unprocessed protein to run a few kDa higher (around 50 kDa). TGFB1 also heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.

    Target

    • Function

      Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
    • Tissue specificity

      Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
    • Involvement in disease

      Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
    • Sequence similarities

      Belongs to the TGF-beta family.
    • Post-translational
      modifications

      Glycosylated.
      The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession P01137 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 282089 Cow
      • Entrez Gene: 7040 Human
      • Omim: 190180 Human
      • SwissProt: P18341 Cow
      • SwissProt: P01137 Human
      • Unigene: 645227 Human
      • Alternative names

        • Cartilage-inducing factor antibody
        • CED antibody
        • Differentiation inhibiting factor antibody
        • DPD1 antibody
        • LAP antibody
        • Latency-associated peptide antibody
        • Prepro transforming growth factor beta 1 antibody
        • TGF beta 1 antibody
        • TGF beta antibody
        • TGF beta 1 protein antibody
        • TGF-beta 1 protein antibody
        • TGF-beta-1 antibody
        • TGF-beta-5 antibody
        • TGF-beta1 antibody
        • TGFB antibody
        • Tgfb-1 antibody
        • tgfb1 antibody
        • TGFB1_HUMAN antibody
        • TGFbeta antibody
        • TGFbeta1 antibody
        • Transforming Growth Factor b1 antibody
        • Transforming Growth Factor beta 1 antibody
        • Transforming growth factor beta 1a antibody
        • transforming growth factor beta-1 antibody
        • transforming growth factor, beta 1 antibody
        • Transforming Growth Factor-ß1 antibody
        see all

      Images

      • Western blot - Anti-TGF beta 1 antibody (ab9758)
        Western blot - Anti-TGF beta 1 antibody (ab9758)
        All lanes : Anti-TGF beta 1 antibody (ab9758) at 1/200 dilution

        Lane 1 : Reduced human recombinant TGF beta 1
        Lane 2 : Non-reduced human recombinant TGF beta 1

        Lysates/proteins at 0.25 µg per lane.

        Secondary
        All lanes : Peroxidase-conjugated anti-rabbit IgG at 1/40000 dilution

        Observed band size: 12,25 kDa
        why is the actual band size different from the predicted?



        Incubated with the primary antibody overnight at 4°C.

        Incubated with the secondary antibody for 30 min at room temperature.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (45)

      Publishing research using ab9758? Please let us know so that we can cite the reference in this datasheet.

      ab9758 has been referenced in 45 publications.

      • Singh S  et al. Endothelial-specific Loss of IFT88 Promotes Endothelial-to-Mesenchymal Transition and Exacerbates Bleomycin-induced Pulmonary Fibrosis. Sci Rep 10:4466 (2020). PubMed: 32161282
      • Jiang X  et al. LncRNA WT1-AS over-expression inhibits non-small cell lung cancer cell stemness by down-regulating TGF-ß1. BMC Pulm Med 20:113 (2020). PubMed: 32349718
      • Yun IS  et al. Effect of Relaxin Expression from an Alginate Gel-Encapsulated Adenovirus on Scar Remodeling in a Pig Model. Yonsei Med J 60:854-863 (2019). PubMed: 31433583
      • Huo Y  et al. LncRNA AWPPH participates in the metastasis of non-small cell lung cancer by upregulating TGF-ß1 expression. Oncol Lett 18:4246-4252 (2019). PubMed: 31516619
      • Oh E  et al. Regulatory T Cells Induce Metastasis by Activating Tgf-? and Enhancing the Epithelial-Mesenchymal Transition. Cells 8:N/A (2019). PubMed: 31690033
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 26 Abreviews or Q&A

      Question

      Customer inquiring about expected band size fo TGF beta 1 protein.
      Molecular weight: 45 kDa

      Read More

      Abcam community

      Verified customer

      Asked on Nov 26 2012

      Answer

      Thank you for contacting Abcam.

      We spoke earlier regarding the expected western blot band size associated with TGF beta-1, detected by antibody ab64715. As we discussed, the molecular weight of the TGF beta-1 protein is about 45 kDa, which is the predicted band size for this antibody. Below is the SwissProt link that I used to obtain the molecular weight and disulfide bond information for the TGF beta-1 protein
      http://www.uniprot.org/uniprot/P01137

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

      Read More

      Abcam Scientific Support

      Answered on Nov 27 2012

      Question

      Coul you suggest antibodies for TGF beat 1, Ki67 anf TGGS protein?

      Read More

      Abcam community

      Verified customer

      Asked on Oct 09 2012

      Answer

      Thank you for contacting us.

      We do not have mouse monoclonal antibodies to the target you are requested. However we do have following antibodies available;

      ab9758; https://www.abcam.com/TGF-beta-1-antibody-ab9758.html

      ab46780; https://www.abcam.com/TGF-beta-1-antibody-ab46780.html

      The immunogen for ab15580 is homologous to rabbit species so this antibody might cross react;

      https://www.abcam.com/Ki67-antibody-Proliferation-Marker-ab15580.html

      I am sorry I am not able to find the antibodies against TGGS.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Oct 09 2012

      Question

      Hola,

      Una pregunta: cuando llegará el kit???

      Gracias

      Read More

      Abcam community

      Verified customer

      Asked on Sep 13 2012

      Answer

      Thank for answering the questions of the Abcam "post complaint survey".

      I understand that you have not used the replacement kit yet.
      I would like to remind you that this free of charge replacement kit is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

      Read More

      Abcam Scientific Support

      Answered on Sep 13 2012

      Question

      I just wanted to thank you again for you incredible help

      Such an amazing service and responsibility for your products



      I feel like I'm in good hands!

      Thanks again and have a great day.

      Read More

      Abcam community

      Verified customer

      Asked on Jul 19 2012

      Answer

      Thank you for your feedback! We are always happy to help, and if you have any further questions please let me know.

      Read More

      Abcam Scientific Support

      Answered on Jul 19 2012

      Question

      Adjunto el cuestionario. Muchas gracias.

      Read More

      Abcam community

      Verified customer

      Asked on Jul 17 2012

      Answer

      Gracias por tomarte la molestia de responder nuestro cuestionario.

      He estudiado el protocolo enviado, y hay una serie de sugerencias que me gustaría hacer para intentar mejorar los resultados del anticuerpo.

      Es posible que no se observen bandas al incubar con el anticuerpo debido a un exceso en las condiciones de bloqueo. Mi recomendación es usar 5% BSA para bloquear en lugar de leche. Incubar durante 1 hora a TA con este agente. Además, para diluir los anticuerpos (primario y secundario) yo os sugeriría usar el mismo agente que para bloquear, es decir BSA, pero a una concentración menor, como 3%.
      Aumentar la concentración de primario, a 1/100.
      Solo por confirmar, ¿es el secundario es especifico contra rabbit?



      Te agradecería que por favor me enviaras los datos del pedido y lote del anticuerpo, pues nos serán útiles para comprobar que no ha habido problemas durante el envío, así como monitorizar la calidad del lote específico de anticuerpo.

      Espero que estos consejos te permitan obtener mejores resultados del anticuerpo. En caso de que no sea así, por favor, no dudes en contactarnos de nuevo para que podamos ayudarte a resolver el problema lo más rápido y eficazmente posible.

      Read More

      Abcam Scientific Support

      Answered on Jul 17 2012

      Question

      Apreciada Abcam
      He comprado un anticuerpos y no funciona.
      Adjunto foto para combrobar lo que pasò.
      El anticuerpo es Tgf beta 1 de tamaño 12-25 KDa.
      Y lo que pasa en WB es que me sale una banda que no es la que yo quieria, mientras en coloración con Comassie la banda es muy clara, y es de 12KDa.
      Puede aiudarme?
      Gracias

      Read More

      Abcam community

      Verified customer

      Asked on Jul 16 2012

      Answer

      Gracias por ponerte en contacto con nosotros y alertarnos del problema experimentado con nuestro producto. Cualquier comentario o queja procedente de nuestros clientes es muy importante para mejorar y monitorizar la calidad de nuestros productos.

      Para tratar de entender mejor el problema, y monitorizar la calidad de nuestros productos, te agradecería mucho que me ampliarais la información del protocolo y las condiciones en las que se ha testado el producto. Te envío un cuestionario que contiene algunas preguntas, y que no se tarda mucho en rellenar, y sin embargo a nosotros nos ayuda a llevar el registro de calidad de los productos y a sugeriros posibles mejoras en caso de encontrarlas.

      Nuestra garantía Abpromise garantiza que los productos funcionan tal y como se especifica en su ficha técnica, y en caso contrario se os manda un anticuerpo de remplazo, se os reembolsa el dinero, o se emite un vale sin fecha de caducidad para futuras compras con nosotros.

      Espero que esta información sea de utilidad. Por favor no dudes en contactarnos para cualquier otra duda o sugerencia.

      Read More

      Abcam Scientific Support

      Answered on Jul 16 2012

      Question

      Thank you for all the help. Yes I think it might be good to try out milk also, due to time constraints and just in order to get results we skipped it because I think the ECL blocking kit contains milk. Either way, I will try milk because as you said, the result might be surprisingly good sometimes.

      I would be really greatful if I could try that antibody so you are welcome to send it to:

      It will be interesting to try our protocol with this antibody. Looking forward to it. Thank you for all your help!

      Best regards

      Read More

      Abcam community

      Verified customer

      Asked on May 17 2012

      Answer

      Thank you for confirming those details. I have now arranged for the chicken polyclonal we discussed to be sent out to you at the address specified. The new order number is xxxxx(purchase order number xxxxxx). This should be with you tomorrow.

      Please do let me know how you get on with your further experiments with ab9758 as well as how you find ab46780.

      Until then, I wish you all the best with your writing and research.

      Read More

      Abcam Scientific Support

      Answered on May 17 2012

      Question

      Hello and thank you for your answer!

      Any kind of help is much appriciated, I have filled your questionnaire and I'm attaching it along with 2 images of the wb results. The biggest issue is that we have multiple bands, especially near were our protein of interest should be (12 and 25 kDa). That is why we think that we can see the protein but not be completley sure. I am very busy with my thesis writing right now but will later run a longer electrophoresis in order to get better separation, and also try to use 1:1000 dilution of the prim ab to reduce the smear and perhaps also the multiple bands. Your tips in this matter would be very gladly taken into account. Tissue lysates from our WT mouse shows a band while our knockout mouse (Gai2-/-) seems to lack it (WB pic 2), which is as we have hypothezied.

      Chicken polyclonal ab46780 seems interesting as it binds to the active form of our protein (as the ab we have been using), the other one you mentioned (mouse monoclonal ab64715) binds to the 45kDa inactive form which we are not interested in. I would of course first have to talk to my professor but can you do anything to the price?

      Thank you for you help!

      Best regards

      Read More

      Abcam community

      Verified customer

      Asked on May 17 2012

      Answer

      Thank you for getting back to me with that information and sorry for the delay in getting back to you.

      From the blot you have shared with me, it does indeed look like you are detecting the protein as expected, especially in the human thrombocyte sample. From having reviewed the protocol used to obtain this blot there are a few suggestions I would make which may improve the non-specificity observed. As you have tried BSA and the ECL blocking agent with non-specificity observed, I would suggest also trying 5% milk blocking. This can sometimes have a surprisingly marked effect.

      As you have mentioned, trying a higher dilution of the primary antibody may also help and increasing the eletrophoresis time will allow you to identify the bands of interest more clearly.

      As you have had to put in quite a bit of effort in optimising this antibody I am willing to provide you with the chicken polyclonmal (ab46780) you are interested in free of charge. If you would like for me to do this could you please confirm that this is the address you would like it sent to:


      I look forward to receiving your reply. Until then, I wish you all the best with your thesis writing.

      Read More

      Abcam Scientific Support

      Answered on May 17 2012

      Question

      Hi!

      I am sorry that I haven't contacted you with a follow-up, I have been very busy with my thesis writing and experiment. We are having some difficulties but we think that we see our protein of interest. It is difficult to interpret since the antibody binds to so many things and it is not specific against only the active form of TGF-beta1. However, we will most probably continue with optimization.

      Your support was very much appriciated due to the quick answers, the tips were however not helpful in this case but we of course understand that in the case of western blot it is really difficult to know what will help and what not. We have some stuff we are going to try and I am positive that we will be able to detect the protein.

      Read More

      Abcam community

      Verified customer

      Asked on May 14 2012

      Answer

      Thank you for getting back to me with how you have been getting on with the anti-TGF beta 1 antibody(ab9758).

      I am glad to hear that you thinkyou have been detecting the expected protein. If you would like help in optimising the experiment further in order to reduce the background observed I'd be happy to help. If you would like me to do this please could you fill out the questionnaire I have attached to this email to find out the up-to-date results and protocol used. Please could you also attach an image of the results obtained.

      As you have been having such problems in getting this antibody to produce good results I would be able to provide you with an alternative antibody from our catalogue if you would like to try one, such as mouse monoclonal ab64715 or chicken polyclonal ab46780. If you would likefor me to arrangethis pleasedo let me know.

      I look forward to receiving your reply.

      Read More

      Abcam Scientific Support

      Answered on May 14 2012

      Question

      Could you please let me know whether this antibody

      Anti-TGF beta 1 antibody (ab9758)

      reacts with rat and opossum tissue?

      Many thanks,

      Read More

      Abcam community

      Verified customer

      Asked on May 07 2012

      Answer

      Thank you very much for your interest in ab9758.

      Unfortunately, this antibody has not been tested in opossum and the immunogen also does not show a significant homology to opossum according to NCBI Blast.

      But rat shows a 100% sequence homology with the immunogen.

      Therefore, I can offer a discount off a future purchase if you buy ab9758 now, test it in rat and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know that you would like to proceed and test ab9758 in rat. I will then send a discount code. This code must be issued before purchasing ab9758 so please wait for my reply before ordering.

      2. Purchase ab9758 either by phone, fax, or online (www.abcam.com).

      3. Test it in rat.

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab9758 turns out to be unsuitable for rat, you will still receive the discount on your next purchase after your Abreview has been submitted.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on May 07 2012

      1-10 of 26 Abreviews or Q&A

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