Anti-TGF beta 1 antibody (ab9758)

Reviews (2)Q&A (24)References (37)

Overview

  • Product name

    Anti-TGF beta 1 antibody
    See all TGF beta 1 primary antibodies

  • Description

    Rabbit polyclonal to TGF beta 1

  • Host species

    Rabbit

  • Specificity

    Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Sheep, Rabbit, Horse, Guinea pig, Hamster, Cow, PigDoes not react with: Dog

  • Immunogen

    Synthetic peptide corresponding to Human TGF beta 1.
    Sequence:

    FSSTEKN-C

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Recombinant human TGF beta 1.

Properties

Applications

Our Abpromise guarantee covers the use of ab9758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 25421510
IHC-P Use at an assay dependent concentration. PubMed: 25207642
WB 1/100 - 1/500.

In reduced samples, the expected bands are 12 kDa (mature protein) and 44 kDa (unprocessed protein). Expected band at 25 kDa in non-reduced samples (homodimer of mature protein). Glycosylations may also cause unprocessed protein to run a few kDa higher (around 50 kDa). TGFB1 also heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.

Target

  • Function

    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.

  • Tissue specificity

    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.

  • Involvement in disease

    Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.

  • Sequence similarities

    Belongs to the TGF-beta family.

  • Post-translational
    modifications

    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Cartilage-inducing factor antibody
    • CED antibody
    • Differentiation inhibiting factor antibody
    • DPD1 antibody
    • LAP antibody
    • Latency-associated peptide antibody
    • Prepro transforming growth factor beta 1 antibody
    • TGF beta 1 antibody
    • TGF beta antibody
    • TGF beta 1 protein antibody
    • TGF-beta 1 protein antibody
    • TGF-beta-1 antibody
    • TGF-beta-5 antibody
    • TGF-beta1 antibody
    • TGFB antibody
    • Tgfb-1 antibody
    • tgfb1 antibody
    • TGFB1_HUMAN antibody
    • TGFbeta antibody
    • TGFbeta1 antibody
    • Transforming Growth Factor b1 antibody
    • Transforming Growth Factor beta 1 antibody
    • Transforming growth factor beta 1a antibody
    • transforming growth factor beta-1 antibody
    • transforming growth factor, beta 1 antibody
    • Transforming Growth Factor-ß1 antibody
    see all

Images

  • Western blot - Anti-TGF beta 1 antibody (ab9758)
    Western blot - Anti-TGF beta 1 antibody (ab9758)
    All lanes : Anti-TGF beta 1 antibody (ab9758) at 1/200 dilution

    Lane 1 : Reduced human recombinant TGF beta 1
    Lane 2 : Non-reduced human recombinant TGF beta 1

    Lysates/proteins at 0.25 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated anti-rabbit IgG at 1/40000 dilution

    Observed band size: 12,25 kDa
    why is the actual band size different from the predicted?



    Incubated with the primary antibody overnight at 4°C.

    Incubated with the secondary antibody for 30 min at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 1 antibody (ab9758)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 1 antibody (ab9758)Image from Young VJ et al., PLoS One. 2014;9(9):e106773. Fig 2.; doi: 10.1371/journal.pone.0106773. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Five micron sections of paraffin embedded peritoneum tissue were mounted onto electrostatically charged microscope slides and dewaxed in xylene (2×5min) and rehydrated. Antigens were retrieval by pressure-cooking slides in 10 mM Tris 1 mM EDTA pH 9, for 5 mins. Slides were washed in H2O before incubation with 3% hydrogen peroxide for 30 min. Slides were blocked then incubated with ab9758 diluted 1/1000 or rabbit IgG in blocking buffer overnight at 4°C. Slides were washed in TBST20 and incubated for 30 min with secondary antibody and washed again before incubation with 3, 3′-diaminobenzidine for 5 min. Slides were then counterstained with hematoxylin, dehydrated and visualized by light microscopy, using an Olympus Provis microscope equipped with a Kodak DCS330 camera.

References

This product has been referenced in:

  • Miao F  et al. LncRNA HAND2-AS1 inhibits non-small cell lung cancer migration, invasion and maintains cell stemness through the interactions with TGF-ß1. Biosci Rep 39:N/A (2019). Read more (PubMed: 30509963) »
  • Xu Q  et al. All-trans retinoic acid inhibits lipopolysaccharide-induced inflammatory responses in bovine adipocytes via TGFß1/Smad3 signaling pathway. BMC Vet Res 15:48 (2019). Read more (PubMed: 30709353) »
See all 37 Publications for this product

Publishing research using ab9758? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 26 Abreviews or Q&A

Question

Customer inquiring about expected band size fo TGF beta 1 protein.
Molecular weight: 45 kDa

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Answer

Thank you for contacting Abcam.

We spoke earlier regarding the expected western blot band size associated with TGF beta-1, detected by antibody ab64715. As we discussed, the molecular weight of the TGF beta-1 protein is about 45 kDa, which is the predicted band size for this antibody. Below is the SwissProt link that I used to obtain the molecular weight and disulfide bond information for the TGF beta-1 protein
http://www.uniprot.org/uniprot/P01137

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

Coul you suggest antibodies for TGF beat 1, Ki67 anf TGGS protein?

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Answer

Thank you for contacting us.

We do not have mouse monoclonal antibodies to the target you are requested. However we do have following antibodies available;

ab9758; https://www.abcam.com/TGF-beta-1-antibody-ab9758.html

ab46780; https://www.abcam.com/TGF-beta-1-antibody-ab46780.html

The immunogen for ab15580 is homologous to rabbit species so this antibody might cross react;

https://www.abcam.com/Ki67-antibody-Proliferation-Marker-ab15580.html

I am sorry I am not able to find the antibodies against TGGS.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Hola,

Una pregunta: cuando llegará el kit???

Gracias

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Answer

Thank for answering the questions of the Abcam "post complaint survey".

I understand that you have not used the replacement kit yet.
I would like to remind you that this free of charge replacement kit is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Question

I just wanted to thank you again for you incredible help

Such an amazing service and responsibility for your products



I feel like I'm in good hands!

Thanks again and have a great day.

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Answer

Thank you for your feedback! We are always happy to help, and if you have any further questions please let me know.

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Question

Adjunto el cuestionario. Muchas gracias.

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Answer

Gracias por tomarte la molestia de responder nuestro cuestionario.

He estudiado el protocolo enviado, y hay una serie de sugerencias que me gustaría hacer para intentar mejorar los resultados del anticuerpo.

Es posible que no se observen bandas al incubar con el anticuerpo debido a un exceso en las condiciones de bloqueo. Mi recomendación es usar 5% BSA para bloquear en lugar de leche. Incubar durante 1 hora a TA con este agente. Además, para diluir los anticuerpos (primario y secundario) yo os sugeriría usar el mismo agente que para bloquear, es decir BSA, pero a una concentración menor, como 3%.
Aumentar la concentración de primario, a 1/100.
Solo por confirmar, ¿es el secundario es especifico contra rabbit?



Te agradecería que por favor me enviaras los datos del pedido y lote del anticuerpo, pues nos serán útiles para comprobar que no ha habido problemas durante el envío, así como monitorizar la calidad del lote específico de anticuerpo.

Espero que estos consejos te permitan obtener mejores resultados del anticuerpo. En caso de que no sea así, por favor, no dudes en contactarnos de nuevo para que podamos ayudarte a resolver el problema lo más rápido y eficazmente posible.

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Question

Apreciada Abcam
He comprado un anticuerpos y no funciona.
Adjunto foto para combrobar lo que pasò.
El anticuerpo es Tgf beta 1 de tamaño 12-25 KDa.
Y lo que pasa en WB es que me sale una banda que no es la que yo quieria, mientras en coloración con Comassie la banda es muy clara, y es de 12KDa.
Puede aiudarme?
Gracias

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Answer

Gracias por ponerte en contacto con nosotros y alertarnos del problema experimentado con nuestro producto. Cualquier comentario o queja procedente de nuestros clientes es muy importante para mejorar y monitorizar la calidad de nuestros productos.

Para tratar de entender mejor el problema, y monitorizar la calidad de nuestros productos, te agradecería mucho que me ampliarais la información del protocolo y las condiciones en las que se ha testado el producto. Te envío un cuestionario que contiene algunas preguntas, y que no se tarda mucho en rellenar, y sin embargo a nosotros nos ayuda a llevar el registro de calidad de los productos y a sugeriros posibles mejoras en caso de encontrarlas.

Nuestra garantía Abpromise garantiza que los productos funcionan tal y como se especifica en su ficha técnica, y en caso contrario se os manda un anticuerpo de remplazo, se os reembolsa el dinero, o se emite un vale sin fecha de caducidad para futuras compras con nosotros.

Espero que esta información sea de utilidad. Por favor no dudes en contactarnos para cualquier otra duda o sugerencia.

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Question

Thank you for all the help. Yes I think it might be good to try out milk also, due to time constraints and just in order to get results we skipped it because I think the ECL blocking kit contains milk. Either way, I will try milk because as you said, the result might be surprisingly good sometimes.

I would be really greatful if I could try that antibody so you are welcome to send it to:

It will be interesting to try our protocol with this antibody. Looking forward to it. Thank you for all your help!

Best regards

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Answer

Thank you for confirming those details. I have now arranged for the chicken polyclonal we discussed to be sent out to you at the address specified. The new order number is xxxxx(purchase order number xxxxxx). This should be with you tomorrow.

Please do let me know how you get on with your further experiments with ab9758 as well as how you find ab46780.

Until then, I wish you all the best with your writing and research.

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Question

Hello and thank you for your answer!

Any kind of help is much appriciated, I have filled your questionnaire and I'm attaching it along with 2 images of the wb results. The biggest issue is that we have multiple bands, especially near were our protein of interest should be (12 and 25 kDa). That is why we think that we can see the protein but not be completley sure. I am very busy with my thesis writing right now but will later run a longer electrophoresis in order to get better separation, and also try to use 1:1000 dilution of the prim ab to reduce the smear and perhaps also the multiple bands. Your tips in this matter would be very gladly taken into account. Tissue lysates from our WT mouse shows a band while our knockout mouse (Gai2-/-) seems to lack it (WB pic 2), which is as we have hypothezied.

Chicken polyclonal ab46780 seems interesting as it binds to the active form of our protein (as the ab we have been using), the other one you mentioned (mouse monoclonal ab64715) binds to the 45kDa inactive form which we are not interested in. I would of course first have to talk to my professor but can you do anything to the price?

Thank you for you help!

Best regards

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Answer

Thank you for getting back to me with that information and sorry for the delay in getting back to you.

From the blot you have shared with me, it does indeed look like you are detecting the protein as expected, especially in the human thrombocyte sample. From having reviewed the protocol used to obtain this blot there are a few suggestions I would make which may improve the non-specificity observed. As you have tried BSA and the ECL blocking agent with non-specificity observed, I would suggest also trying 5% milk blocking. This can sometimes have a surprisingly marked effect.

As you have mentioned, trying a higher dilution of the primary antibody may also help and increasing the eletrophoresis time will allow you to identify the bands of interest more clearly.

As you have had to put in quite a bit of effort in optimising this antibody I am willing to provide you with the chicken polyclonmal (ab46780) you are interested in free of charge. If you would like for me to do this could you please confirm that this is the address you would like it sent to:


I look forward to receiving your reply. Until then, I wish you all the best with your thesis writing.

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Question

Hi!

I am sorry that I haven't contacted you with a follow-up, I have been very busy with my thesis writing and experiment. We are having some difficulties but we think that we see our protein of interest. It is difficult to interpret since the antibody binds to so many things and it is not specific against only the active form of TGF-beta1. However, we will most probably continue with optimization.

Your support was very much appriciated due to the quick answers, the tips were however not helpful in this case but we of course understand that in the case of western blot it is really difficult to know what will help and what not. We have some stuff we are going to try and I am positive that we will be able to detect the protein.

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Answer

Thank you for getting back to me with how you have been getting on with the anti-TGF beta 1 antibody(ab9758).

I am glad to hear that you thinkyou have been detecting the expected protein. If you would like help in optimising the experiment further in order to reduce the background observed I'd be happy to help. If you would like me to do this please could you fill out the questionnaire I have attached to this email to find out the up-to-date results and protocol used. Please could you also attach an image of the results obtained.

As you have been having such problems in getting this antibody to produce good results I would be able to provide you with an alternative antibody from our catalogue if you would like to try one, such as mouse monoclonal ab64715 or chicken polyclonal ab46780. If you would likefor me to arrangethis pleasedo let me know.

I look forward to receiving your reply.

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Question

Could you please let me know whether this antibody

Anti-TGF beta 1 antibody (ab9758)

reacts with rat and opossum tissue?

Many thanks,

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Answer

Thank you very much for your interest in ab9758.

Unfortunately, this antibody has not been tested in opossum and the immunogen also does not show a significant homology to opossum according to NCBI Blast.

But rat shows a 100% sequence homology with the immunogen.

Therefore, I can offer a discount off a future purchase if you buy ab9758 now, test it in rat and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab9758 in rat. I will then send a discount code. This code must be issued before purchasing ab9758 so please wait for my reply before ordering.

2. Purchase ab9758 either by phone, fax, or online (www.abcam.com).

3. Test it in rat.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab9758 turns out to be unsuitable for rat, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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1-10 of 26 Abreviews or Q&A

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