Key features and details
- Rabbit polyclonal to TGF beta 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TGF beta 1 antibody
See all TGF beta 1 primary antibodies
DescriptionRabbit polyclonal to TGF beta 1
SpecificityFull length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Sheep, Rabbit, Horse, Guinea pig, Hamster, Cow, PigDoes not react with: Dog
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab9758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500.
In reduced samples, the expected bands are 12 kDa (mature protein) and 44 kDa (unprocessed protein). Expected band at 25 kDa in non-reduced samples (homodimer of mature protein). Glycosylations may also cause unprocessed protein to run a few kDa higher (around 50 kDa). TGFB1 also heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
Sequence similaritiesBelongs to the TGF-beta family.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
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All lanes : Anti-TGF beta 1 antibody (ab9758) at 1/200 dilution
Lane 1 : Reduced human recombinant TGF beta 1
Lane 2 : Non-reduced human recombinant TGF beta 1
Lysates/proteins at 0.25 µg per lane.
All lanes : Peroxidase-conjugated anti-rabbit IgG at 1/40000 dilution
Observed band size: 12,25 kDa why is the actual band size different from the predicted?
Incubated with the primary antibody overnight at 4°C.
Incubated with the secondary antibody for 30 min at room temperature.
ab9758 has been referenced in 45 publications.
- Singh S et al. Endothelial-specific Loss of IFT88 Promotes Endothelial-to-Mesenchymal Transition and Exacerbates Bleomycin-induced Pulmonary Fibrosis. Sci Rep 10:4466 (2020). PubMed: 32161282
- Jiang X et al. LncRNA WT1-AS over-expression inhibits non-small cell lung cancer cell stemness by down-regulating TGF-ß1. BMC Pulm Med 20:113 (2020). PubMed: 32349718
- Yun IS et al. Effect of Relaxin Expression from an Alginate Gel-Encapsulated Adenovirus on Scar Remodeling in a Pig Model. Yonsei Med J 60:854-863 (2019). PubMed: 31433583
- Huo Y et al. LncRNA AWPPH participates in the metastasis of non-small cell lung cancer by upregulating TGF-ß1 expression. Oncol Lett 18:4246-4252 (2019). PubMed: 31516619
- Oh E et al. Regulatory T Cells Induce Metastasis by Activating Tgf-? and Enhancing the Epithelial-Mesenchymal Transition. Cells 8:N/A (2019). PubMed: 31690033