Anti-TGF beta 1 antibody (ab9758)

Thank you for contacting Abcam.

We spoke earlier regarding the expected western blot band size associated with TGF beta-1, detected by antibody ab64715. As we discussed, the molecular weight of the TGF beta-1 protein is about 45 kDa, which is the predicted band size for this antibody. Below is the SwissProt link that I used to obtain the molecular weight and disulfide bond information for the TGF beta-1 protein
http://www.uniprot.org/uniprot/P01137

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Thank you for contacting us.

We do not have mouse monoclonal antibodies to the target you are requested. However we do have following antibodies available;

ab9758; https://www.abcam.com/TGF-beta-1-antibody-ab9758.html

ab46780; https://www.abcam.com/TGF-beta-1-antibody-ab46780.html

The immunogen for ab15580 is homologous to rabbit species so this antibody might cross react;

https://www.abcam.com/Ki67-antibody-Proliferation-Marker-ab15580.html

I am sorry I am not able to find the antibodies against TGGS.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

Thank for answering the questions of the Abcam "post complaint survey".

I understand that you have not used the replacement kit yet.
I would like to remind you that this free of charge replacement kit is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for your feedback! We are always happy to help, and if you have any further questions please let me know.

Gracias por tomarte la molestia de responder nuestro cuestionario.

He estudiado el protocolo enviado, y hay una serie de sugerencias que me gustaría hacer para intentar mejorar los resultados del anticuerpo.

Es posible que no se observen bandas al incubar con el anticuerpo debido a un exceso en las condiciones de bloqueo. Mi recomendación es usar 5% BSA para bloquear en lugar de leche. Incubar durante 1 hora a TA con este agente. Además, para diluir los anticuerpos (primario y secundario) yo os sugeriría usar el mismo agente que para bloquear, es decir BSA, pero a una concentración menor, como 3%.
Aumentar la concentración de primario, a 1/100.
Solo por confirmar, ¿es el secundario es especifico contra rabbit?



Te agradecería que por favor me enviaras los datos del pedido y lote del anticuerpo, pues nos serán útiles para comprobar que no ha habido problemas durante el envío, así como monitorizar la calidad del lote específico de anticuerpo.

Espero que estos consejos te permitan obtener mejores resultados del anticuerpo. En caso de que no sea así, por favor, no dudes en contactarnos de nuevo para que podamos ayudarte a resolver el problema lo más rápido y eficazmente posible.

Gracias por ponerte en contacto con nosotros y alertarnos del problema experimentado con nuestro producto. Cualquier comentario o queja procedente de nuestros clientes es muy importante para mejorar y monitorizar la calidad de nuestros productos.

Para tratar de entender mejor el problema, y monitorizar la calidad de nuestros productos, te agradecería mucho que me ampliarais la información del protocolo y las condiciones en las que se ha testado el producto. Te envío un cuestionario que contiene algunas preguntas, y que no se tarda mucho en rellenar, y sin embargo a nosotros nos ayuda a llevar el registro de calidad de los productos y a sugeriros posibles mejoras en caso de encontrarlas.

Nuestra garantía Abpromise garantiza que los productos funcionan tal y como se especifica en su ficha técnica, y en caso contrario se os manda un anticuerpo de remplazo, se os reembolsa el dinero, o se emite un vale sin fecha de caducidad para futuras compras con nosotros.

Espero que esta información sea de utilidad. Por favor no dudes en contactarnos para cualquier otra duda o sugerencia.

Thank you for confirming those details. I have now arranged for the chicken polyclonal we discussed to be sent out to you at the address specified. The new order number is xxxxx(purchase order number xxxxxx). This should be with you tomorrow.

Please do let me know how you get on with your further experiments with ab9758 as well as how you find ab46780.

Until then, I wish you all the best with your writing and research.

Thank you for getting back to me with that information and sorry for the delay in getting back to you.

From the blot you have shared with me, it does indeed look like you are detecting the protein as expected, especially in the human thrombocyte sample. From having reviewed the protocol used to obtain this blot there are a few suggestions I would make which may improve the non-specificity observed. As you have tried BSA and the ECL blocking agent with non-specificity observed, I would suggest also trying 5% milk blocking. This can sometimes have a surprisingly marked effect.

As you have mentioned, trying a higher dilution of the primary antibody may also help and increasing the eletrophoresis time will allow you to identify the bands of interest more clearly.

As you have had to put in quite a bit of effort in optimising this antibody I am willing to provide you with the chicken polyclonmal (ab46780) you are interested in free of charge. If you would like for me to do this could you please confirm that this is the address you would like it sent to:


I look forward to receiving your reply. Until then, I wish you all the best with your thesis writing.

Thank you for getting back to me with how you have been getting on with the anti-TGF beta 1 antibody(ab9758).

I am glad to hear that you thinkyou have been detecting the expected protein. If you would like help in optimising the experiment further in order to reduce the background observed I'd be happy to help. If you would like me to do this please could you fill out the questionnaire I have attached to this email to find out the up-to-date results and protocol used. Please could you also attach an image of the results obtained.

As you have been having such problems in getting this antibody to produce good results I would be able to provide you with an alternative antibody from our catalogue if you would like to try one, such as mouse monoclonal ab64715 or chicken polyclonal ab46780. If you would likefor me to arrangethis pleasedo let me know.

I look forward to receiving your reply.

Thank you very much for your interest in ab9758.

Unfortunately, this antibody has not been tested in opossum and the immunogen also does not show a significant homology to opossum according to NCBI Blast.

But rat shows a 100% sequence homology with the immunogen.

Therefore, I can offer a discount off a future purchase if you buy ab9758 now, test it in rat and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab9758 in rat. I will then send a discount code. This code must be issued before purchasing ab9758 so please wait for my reply before ordering.

2. Purchase ab9758 either by phone, fax, or online (www.abcam.com).

3. Test it in rat.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab9758 turns out to be unsuitable for rat, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.