Anti-TGF beta 1 antibody (ab9758)
- Datasheet
- References (23)
- Protocols
Overview
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Product nameAnti-TGF beta 1 antibody
See all TGF beta 1 primary antibodies -
DescriptionRabbit polyclonal to TGF beta 1
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Host speciesRabbit
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SpecificityFull length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
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Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Sheep, Rabbit, Horse, Guinea pig, Hamster, Cow, Pig
Does not react with:
Dog -
Immunogen
Synthetic peptide corresponding to Human TGF beta 1.
Sequence:FSSTEKN-C
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Positive control
- WB: Recombinant human TGF beta 1.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab9758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. PubMed: 25421510 | |
| IHC-P | Use at an assay dependent concentration. PubMed: 25207642 | |
| WB | 1/100 - 1/500. In reduced samples, the expected bands are 12 kDa (mature protein) and 44 kDa (unprocessed protein). Expected band at 25 kDa in non-reduced samples (homodimer of mature protein). Glycosylations may also cause unprocessed protein to run a few kDa higher (around 50 kDa). TGFB1 also heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB. |
Target
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FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
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Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
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Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
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Sequence similaritiesBelongs to the TGF-beta family.
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Post-translational
modificationsGlycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. -
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
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Database links
- Entrez Gene: 282089 Cow
- Entrez Gene: 7040 Human
- Entrez Gene: 21803 Mouse
- Omim: 190180 Human
- SwissProt: P18341 Cow
- SwissProt: P01137 Human
- SwissProt: P04202 Mouse
- Unigene: 645227 Human
see all -
Alternative names
- Cartilage-inducing factor antibody
- CED antibody
- Differentiation inhibiting factor antibody
see all
Images
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Western blot - Anti-TGF beta 1 antibody (ab9758)All lanes : Anti-TGF beta 1 antibody (ab9758) at 1/200 dilution
Lane 1 : Reduced recombinant TGF beta 1
Lane 2 : Non-reduced recombinant TGF beta 1
Lysates/proteins at 0.25 µg per lane.
Secondary
All lanes : Peroxidase-conjugated anti-rabbit IgG at 1/40000 dilution
Observed band size: 12,25 kDa why is the actual band size different from the predicted?Incubated with the primary antibody overnight at 4°C.
Incubated with the secondary antibody for 30 min at room temperature.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 1 antibody (ab9758)Image from Young VJ et al., PLoS One. 2014;9(9):e106773. Fig 2.; doi: 10.1371/journal.pone.0106773.Five micron sections of paraffin embedded peritoneum tissue were mounted onto electrostatically charged microscope slides and dewaxed in xylene (2×5min) and rehydrated. Antigens were retrieval by pressure-cooking slides in 10 mM Tris 1 mM EDTA pH 9, for 5 mins. Slides were washed in H2O before incubation with 3% hydrogen peroxide for 30 min. Slides were blocked then incubated with ab9758 diluted 1/1000 or rabbit IgG in blocking buffer overnight at 4°C. Slides were washed in TBST20 and incubated for 30 min with secondary antibody and washed again before incubation with 3, 3′-diaminobenzidine for 5 min. Slides were then counterstained with hematoxylin, dehydrated and visualized by light microscopy, using an Olympus Provis microscope equipped with a Kodak DCS330 camera.
Datasheets and documents
References
This product has been referenced in:
- Mendes LO et al. Modulation of inflammatory and hormonal parameters in response to testosterone therapy: Effects on the ventral prostate of adult rats. Cell Biol Int N/A:N/A (2018). Read more (PubMed: 29771451) »
- Murugavel S et al. Valproic Acid Induces Endothelial-to-Mesenchymal Transition-Like Phenotypic Switching. Front Pharmacol 9:737 (2018). Read more (PubMed: 30050438) »
