Recombinant Anti-TGF beta 1 antibody [EPR18163] (ab179695)
![Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)](https://www.abcam.com/ps/products/179/ab179695/Images/ab179695-469676-anti-tgf-beta-1-antibody-epr18163-western-blot-wildtype-a549-tgfb1-knockout-a549-k562-shsy5y.jpg "Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18163] to TGF beta 1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-TGF beta 1 antibody [EPR18163]
See all TGF beta 1 primary antibodies -
Description
Rabbit monoclonal [EPR18163] to TGF beta 1 -
Host species
Rabbit -
Specificity
ab179695 recognizes the mature and cleaved forms of TGF-beta 1. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: L-929, RAW 264.7, HeLa, Wild-type A549, A549, SH-SY5Y and K562 whole cell lysates; Mouse serum; Mouse spleen lysate; Rat kidney and spleen lysates; Rat serum; Human kidney and spleen lysates; Recombinant protein fragment human TGF beta 1.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18163 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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KO cell pellets
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/1000. Detects a band of approximately 50, 12.5 kDa (predicted molecular weight: 44 kDa).
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 50, 12.5 kDa (predicted molecular weight: 44 kDa). |
Target
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Function
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. -
Tissue specificity
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. -
Involvement in disease
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. -
Sequence similarities
Belongs to the TGF-beta family. -
Post-translational
modificationsGlycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 7040 Human
- Entrez Gene: 21803 Mouse
- Entrez Gene: 59086 Rat
- Omim: 190180 Human
- SwissProt: P01137 Human
- SwissProt: P04202 Mouse
- SwissProt: P17246 Rat
- Unigene: 645227 Human
see all -
Alternative names
- Cartilage-inducing factor antibody
- CED antibody
- Differentiation inhibiting factor antibody
see all
Images
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Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)All lanes : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : TGFB1 knockout A549 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab179695 observed at 48 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab179695 was shown to react with TGF beta in wild-type A549 cells in Western blot with loss of signal observed in TGFB1 knockout cell line ab269509 (TGFB1 knockout cell lysate ab269671). Wild-type A549 and TGFB1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab179695 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)](https://www.abcam.com/ps/products/179/ab179695/Images/ab179695-5-anti-tgf-beta-1-antibody-epr18163-western-blot-human-lysate.jpg)
Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)All lanes : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Wild-type HeLa cell lysate
Lane 4 : TGF beta 1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 44 kDaLanes 1 - 4: Merged signal (red and green). Green - ab179695 observed at 50 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab179695 was shown to react with TGF beta 1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255439 (knockout cell lysate ab263799) was used. Wild-type and TGF beta 1 knockout samples were subjected to SDS-PAGE. ab179695 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)](https://www.abcam.com/ps/products/179/ab179695/Images/ab179695-258575-anti-tgf-beta-1-antibody-epr18163-western-blot.jpg)
Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)All lanes : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/5000 dilution
Lane 1 : L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 44 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 minutes; Lane 2-4:30 seconds.
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![Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)](https://www.abcam.com/ps/products/179/ab179695/Images/ab179695-258573-anti-tgf-beta-1-antibody-epr18163-western-blot.jpg)
Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)Lane 1 : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/5000 dilution
Lanes 2-5 : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution
Lane 1 : Mouse serum
Lane 2 : Mouse spleen lysate
Lane 3 : Rat kidney lysate
Lane 4 : Rat spleen lysate
Lane 5 : Rat serum
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 44 kDa
Observed band size: 12.5,50 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/2/3/5: 3minutes; Lane 4: 30 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID:18390240; PMID:12717387; PMID:2139036).
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![Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)](https://www.abcam.com/ps/products/179/ab179695/Images/ab179695-258572-anti-tgf-beta-1-antibody-epr18163-western-blot.jpg)
Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)All lanes : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution
Lane 1 : Human kidney lysate
Lane 2 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
Predicted band size: 44 kDa
Observed band size: 12.5,50 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID:18390240; PMID:12717387; PMID:2139036).
-
![Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)](https://www.abcam.com/ps/products/179/ab179695/Images/ab179695-258570-anti-tgf-beta-1-antibody-epr18163-western-blot.jpg)
Western blot - Anti-TGF beta 1 antibody [EPR18163] (ab179695)All lanes : Anti-TGF beta 1 antibody [EPR18163] (ab179695) at 1/1000 dilution
Lane 1 : Recombinant protein fragment human TGF beta 1
Lane 2 : Recombinant protein fragment human TGF beta 2
Lane 3 : Recombinant protein fragment human TGF beta 3
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 1 second; Lane 2/3: 3 minutes.
Recombinant protein fragment human TGF beta 1 contains aa279-390 with a GST-tag. Recombinant protein fragment human TGF beta 2 contains aa303-414 with a GST-tag Recombinant protein fragment human TGF beta 3 contains aa301-412 with a GST-tag. These three fragment recombinant proteins were made in-house.
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![Anti-TGF beta 1 antibody [EPR18163] (ab179695)](https://www.abcam.com/ps/products/179/ab179695/Images/ab179695-7-benefits-of-recombinant-antibodies.png)
Anti-TGF beta 1 antibody [EPR18163] (ab179695)
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (44)
ab179695 has been referenced in 44 publications.
- Wang Q et al. Association of miRNA-145 with the occurrence and prognosis of hydrosalpinx-induced defective endometrial receptivity. Bosn J Basic Med Sci 21:81-92 (2021). PubMed: 32343942
- Liang Y et al. Silenced SOX2-OT alleviates ventricular arrhythmia associated with heart failure by inhibiting NLRP3 expression via regulating miR-2355-3p. Immun Inflamm Dis 9:255-264 (2021). PubMed: 33270361
- Wei X et al. Ultrasound-targeted microbubble destruction-mediated Galectin-7-siRNA promotes the homing of bone marrow mesenchymal stem cells to alleviate acute myocardial infarction in rats. Int J Mol Med 47:677-687 (2021). PubMed: 33416139
- Liu X et al. Transcription elongation factor A-like 7, regulated by miR-758-3p inhibits the progression of melanoma through decreasing the expression levels of c-Myc and AKT1. Cancer Cell Int 21:43 (2021). PubMed: 33430878
- Miao J et al. Paeonol attenuates inflammation by confining HMGB1 to the nucleus. J Cell Mol Med 25:2885-2899 (2021). PubMed: 33534963
