Application
Western blot
Sample
Cow Recombinant protein (Total protein extracted from transiently transform)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
7000 µg
Specification
Total protein extracted from transiently transform
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: TBS+ 0.05% sodium azide
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed
Detection
Detection method
BCIP/NBT
Negative control
Untransformed plants, and plants transformed with an empty vector (mock)
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 48 kDa
Positive control
Recombinant Cow TGF beta 1 protein (ab224869) 10ng
Additional data
Additional Notes
Recombinant bovine tgfB1 fusion protein, transiently expressed in Tobacco plants, predicted molecular weight: 48 kD.
There are many other bands in the test samples, perhaps due to PTMs (for band more then 48 kD) and degradation (for less then 48 kD)
There aren't any none-spesific bands in the negative controls (Untransformed plants or plants transformed with an empty vector).
MW Ladder: SuperMarker2700 (5ul)
Samples:
1: 10ng of ab224869
2-7: transformed plants
8, 10: mock transformation
9, 11: wt plants
Abcam user community
Verified customer
Submitted Oct 26 2020