Overview

  • Product name
    Anti-TGF beta 1 antibody [TB21]
    See all TGF beta 1 primary antibodies
  • Description
    Mouse monoclonal [TB21] to TGF beta 1
  • Host species
    Mouse
  • Specificity
    This antibody recognises natural and recombinant TGF beta 1. It reacts with both dimeric and monomeric natural forms under reducing and non reducing conditions. Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, ELISA, ICC/IF, Neutralising, WBmore details
  • Species reactivity
    Reacts with: Sheep, Human, Pig
    Predicted to work with: Rat
  • Immunogen

    Human TGF Beta 1 from human platelets.

  • Positive control
  • General notes

    We have mixed feedback from customers about the Rat specificity so the Rat species has been moved to predicted as we can't guarantee it. The antibody has been successfully used with Rat samples in publication PMID 22471627 and PMID 19820199 though.

     

    This clone (TB21) demonstrates neutralising activity against TGF beta 1 in cell proliferation assays. Removal of sodium azide is recommended prior to use in functional assays.

Properties

Applications

Our Abpromise guarantee covers the use of ab27969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration. PubMed: 8237223
WB 1/2000.

See Abreview. We recommend using BSA in blocking solution. Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.

Target

  • Function
    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • Tissue specificity
    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • Involvement in disease
    Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Post-translational
    modifications
    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cartilage-inducing factor antibody
    • CED antibody
    • Differentiation inhibiting factor antibody
    • DPD1 antibody
    • LAP antibody
    • Latency-associated peptide antibody
    • Prepro transforming growth factor beta 1 antibody
    • TGF beta 1 antibody
    • TGF beta antibody
    • TGF beta 1 protein antibody
    • TGF-beta 1 protein antibody
    • TGF-beta-1 antibody
    • TGF-beta-5 antibody
    • TGF-beta1 antibody
    • TGFB antibody
    • Tgfb-1 antibody
    • tgfb1 antibody
    • TGFB1_HUMAN antibody
    • TGFbeta antibody
    • TGFbeta1 antibody
    • Transforming Growth Factor b1 antibody
    • Transforming Growth Factor beta 1 antibody
    • Transforming growth factor beta 1a antibody
    • transforming growth factor beta-1 antibody
    • transforming growth factor, beta 1 antibody
    • Transforming Growth Factor-ß1 antibody
    see all

Images

  • ab27969 staining TGF beta 1 in rat kidney tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 2% BSA for 2 hours at 25°C followed by incubation with the primary antibody, at a 1/200 dilution, for 9 hours at 4°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 594 (red) used at a 1/500 dilution.

    See Abreview

  • ab 27969 staining TGF beta 1 in human 293ft cells by Immunocytochemistry/ Immunofluorescence.
    The 293 FT cells were cultured for 3 days then fixed with 3.7% formaldehyde for 10 minutes and blocked with 5% BSA in PBS for 1 hour at +4°C. The cells were incubated with the primary antibody at 1/200 dilution overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 555 Goat anti-Rabbit IgG used at a dilution of 1/200.
  • ab27969 staining TGF beta 1 in Human ureter tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/150) for 16 hours at 4°C. A Cy2®-conjugated Goat anti-mouse polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Chen X  et al. The role of miR-497-5p in myofibroblast differentiation of LR-MSCs and pulmonary fibrogenesis. Sci Rep 7:40958 (2017). WB ; Mouse . Read more (PubMed: 28098218) »
  • Chen Y  et al. Degradation of Mg-6Zn alloy stents does not influence the healing of the common bile duct in vivo. Exp Ther Med 13:2651-2656 (2017). Read more (PubMed: 28587326) »
See all 30 Publications for this product

Customer reviews and Q&As

1-10 of 32 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human skin)
Specification
Human skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 31 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Ureter)
Specification
Ureter
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 14 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Ureter)
Specification
Ureter
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C

Ms. Beate Thode

Verified customer

Submitted Mar 14 2013

Answer

Vielen Dank für Ihre Anfrage.

Ich freue mich ihnen mitteilen zu koennen dass wir fuer den gesamten November unsere Aktion 4 fuer 3 laufen haben. Bei der Bestellung von 4 Produkten bekommen Sie das guenstigste Produkt kostenfrei.

Sie koennen ganz einfach per E-mail bestellen: mailto:orders@abcam.com


Geben sie bei der Bestellung bitte den Aktionscode an: 4-FOR-3-EB3AB


Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for getting back to me with that information.

I am not entirely sure why you have lost the staining. Could it be that the level of TGF beta 1 is quite low in your samples? I have found reference to it being quite low in normal rat brain, and elevated by injury:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3054842/pdf/11961311.pdf

From the gene expression database, it seems that the protein has been found to be under-expressed in brain tissue:

http://www.ebi.ac.uk/gxa/gene?gid=P01137

Do you have access to any tissue where the protein would be expressed at a higher level such as spleen, kidneyor bone?

I look forward to receiving your reply.

Read More

Answer

Thank you for getting back to me with that information.

I am sorry to hear that you are still having difficulties with the TGF beta 1 staining. Since your initial complaint we have retested both the lots of ab27969 you used and have not been able to identify a problem. I am therefore not sure where the problems may be originating from.

I have your protocol details that you provided earlier. Could you provide a few more details so I can try to see if there is anything we would possibly suggest changing to improve the results observed so far?

1. Has the way you have collected the samples changed at all since using the first vial of ab27969 which you purchased last year?

2. Have you tried the secondary antibody used with ab27969 and ab64715 with a different primary antibody? How have these results been? Which secondary antibody are you using (Supplier and catalogue number)?

3. Have you stained any other targets on the same tissue preparations?

I will try to have a further look into how these antibodies have been used previously to see if there are any further suggestions I can provide.

I look forward to receiving your reply.

Read More

Answer

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu. Je me permets de vous transférer le numéro de commande de remplacement gratuit de ce produit :xx. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

Veuillez noter que le tube de remplacement est aussi couvert par notre garantie dans le cas ou le problème ne serait pas résolu.

N'hésitez pas à nous contacter lors d'une prochaine occasion.

Read More

Answer

Merci de votre réponse et d'avoir pris en compte mes suggestions. Puisque dans le contrôle positive vous ne voyez pas non plus la bande spécifique mais les autres bandes, je dois conclure que l'anticorps est endommagé. Je suis désolée que il semble que vous avez reçu un tube fautif et je serais ravie de vous remplacer ce tube soit par unnouveau tube de cet anticorps, un autre anticorps ouun avoir/remboursement. Veuillez s'il vous plaît me dire si vous avez des questions ainsi que me confirmer quel option vous préférez..

Je me réjouis de votre réponse.

Read More

Question
Answer

Merci d'avoir pris le temps de remplir notre questionnaire et de nous avoir contactés. Je suis désolée que vous avez eu des difficultés à obtenir de bons résultats cet anticorps.

Comme vous savez, notre politique est d'offrir tout d'abord la meilleure assistance technique. Si nous concluons que le produit que vous avez reçu ne fonctionne pas comme mentionné sur sa fiche technique et que ce produit a été commandé sous 180 jours, nous nous ferons un plaisir de vous offrir un remplacement gratuit ou un avoir en compensation.

J'aimerais donc vous offrir quelques suggestions afin d'optimiser vos résultats. J'apprécierais si vous pouviez confirmer quelques détails de la procédure.

1.) Je suggère fortement de prendre un contrôle positive avec. Ceci va permettre de valider l'expérience ainsi que le poids moléculaire attendue. Comme contrôle positive, vous pouvez prendre tout tissu/cellule avec expression connue. Les cellules MCF7 par exemple expriment le TGF beta 1.

2.) Est-ce que vous avez vérifié que les protéines de bas poids moléculaires se sont bien transférées?

3.) Est-ce que vous avez ajouté un agent dénaturant aux échantillons, comme le SDS? Est-ce que vous avez dilué les échantillons dans le tampon Laemmli par exemple?

4.) Combien d'agent réducteur est-ce que vous avez mis? Est-ce que vous pouvez ajouter encore plus de beta Mercaptoethanol?

J'espère que ces informations vous aideront.Veuillez s'il vous plaît me tenir au courant des résultats. Je me réjouis de votre réponse.

Read More

Answer

Thank you very much for contacting us.

I have looked at the review you have been attempting to submit and I have a few questions.

1. The review xxxxx is for the antibody anti-Osteopontin antibody ab11503, not the anti-TGF beta 1 antibody ab27969. Is this intentional? The review number 31406 is for the anti-TGF beta 1 antibody ab27969, is this the one you have been having difficulty with?

2. The application ofreview xxxx and xxxxx chosen is "blocking", if the review is for "Neutralising"I would choose "other". Can you describe the experiment to me so that I can evaluate if this would be the correct catagory?

I am unsure as to why the system is not letting you change the sample type, but if you can fill in as much as possible then I can change the other information manually for you. If you provide me with the remaining detail of the Abreview I can fill it in for you on my side.

I hope this information has been of help. I look forward to receiving your reply.

Read More

1-10 of 32 Abreviews or Q&A

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