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Hello Could you please clarify the suggested starting dilution for the use of the product AB27969 (TGF-beta1 Mab) for use in IHC on paraffin sections. In the datasheet, it is written as 1:10. Could you please confirm this low dilution? Thank you Kind regards
Asked on Jan 10 2012
Thank you for your enquiry and for your patience. It has taken some time to confirm this information for you with our source. I can confirm that this antibody has been used at 1:1000 successfully. I have added this to the datasheet. However, other researchers have needed to use it at 1:10. The dilutions provided on the online datasheet are a guideline only. Individual customers will usually need to optimize the dilution for their individual experiments. I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.
Answered on Jan 10 2012