Product nameAnti-TGF beta 3 antibody
See all TGF beta 3 primary antibodies
DescriptionRabbit polyclonal to TGF beta 3
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, IPmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Human
Synthetic peptide within Human TGF beta 3 aa 350 to the C-terminus. The exact sequence is proprietary.
Database link: P10600
- Purchase matching WB positive control:Recombinant human TGF beta 3 protein (Active)
- MCF7 lysate, Placenta
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab15537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use at an assay dependent concentration.
The immunogen for ab15537 is located close to C-terminus and therefore should be able to detect the proform at 50 kDa and mature form at 12.5 kDa under reducing condition.
|IHC-P||1/100. Incubate for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. Ab15537 demonstrates cytoplasmic staining.|
|IP||Use at an assay dependent concentration.|
FunctionInvolved in embryogenesis and cell differentiation.
Involvement in diseaseDefects in TGFB3 are a cause of familial arrhythmogenic right ventricular dysplasia type 1 (ARVD1) [MIM:107970]; also known as arrhythmogenic right ventricular cardiomyopathy 1 (ARVC1). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
Sequence similaritiesBelongs to the TGF-beta family.
- Information by UniProt
- ARVD antibody
- ARVD1 antibody
- FLJ16571 antibody
All lanes : Anti-TGF beta 3 antibody (ab15537) at 1/200 dilution
Lane 1 : MCF7 (Hu) whole cell lysate
Lane 2 : U2OS (Hu) whole cell lysate
Lane 3 : 293T (Hu) whole cell lysate
Lane 4 : Cos7 (AGMk) whole cell lysate
Lane 5 : CHO (Hm) whole cell lysate
Lane 6 : PC12 (Rat) whole cell lysate
Lane 7 : 3T3 (Ms) whole cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : HRP conjugated swine anti-rabbit antibody at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 6 minutes
The blot was blocked with 5% milk for 1 hour at 21°C and incubated with the primary antibody for 12 hours at 4°C.
Detects a 50 kDa species, which is the presumed TGF-Beta 3 precursor, across all cell lysates and species tested including: Hu, AGMk Ms, Rat, and Hm. Higher molecular weight species are observed in all lysates, which could be glycosylated forms of the precursor. A fast migrating species (~14 kDa) was observed in U2OS cell lysate (Lane 2), which could be the cleaved, active form of TGF-Beta3.
Human placenta stained with ab15537 by Immunohistochemistry (FFPE-sections).
ab15537 at 1/200 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before incubation with the antibody for 30 minutes. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ICC/IF image of ab15537 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15537 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- MacFarlane EG et al. Lineage-specific events underlie aortic root aneurysm pathogenesis in Loeys-Dietz syndrome. J Clin Invest 129:659-675 (2019). Read more (PubMed: 30614814) »
- He W et al. Synergistic effects of recombinant Lentiviral-mediated BMP2 and TGF-beta3 on the osteogenic differentiation of rat bone marrow mesenchymal stem cells in vitro. Cytokine 120:1-8 (2019). Read more (PubMed: 30991228) »