Key features and details
- Rabbit polyclonal to TGF beta 3
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Rabbit, Human
- Isotype: IgG
Product nameAnti-TGF beta 3 antibody
See all TGF beta 3 primary antibodies
DescriptionRabbit polyclonal to TGF beta 3
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Human
Synthetic peptide within Human TGF beta 3 aa 350 to the C-terminus. The exact sequence is proprietary.
Database link: P10600
- Purchase matching WB positive control:Recombinant human TGF beta 3 protein (Active)
- MCF7 lysate, Placenta
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab15537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/100. Incubate for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. Ab15537 demonstrates cytoplasmic staining.|
FunctionInvolved in embryogenesis and cell differentiation.
Involvement in diseaseDefects in TGFB3 are a cause of familial arrhythmogenic right ventricular dysplasia type 1 (ARVD1) [MIM:107970]; also known as arrhythmogenic right ventricular cardiomyopathy 1 (ARVC1). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
Sequence similaritiesBelongs to the TGF-beta family.
- Information by UniProt
- ARVD antibody
- ARVD1 antibody
- FLJ16571 antibody
Human placenta stained with ab15537 by Immunohistochemistry (FFPE-sections).
ab15537 at 1/200 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before incubation with the antibody for 30 minutes. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ICC/IF image of ab15537 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15537 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab15537 has been referenced in 28 publications.
- Conedera FM et al. Diverse Signaling by TGFß Isoforms in Response to Focal Injury is Associated with Either Retinal Regeneration or Reactive Gliosis. Cell Mol Neurobiol N/A:N/A (2020). PubMed: 32219603
- Chen X et al. Exosome-mediated transfer of miR-93-5p from cancer-associated fibroblasts confer radioresistance in colorectal cancer cells by downregulating FOXA1 and upregulating TGFB3. J Exp Clin Cancer Res 39:65 (2020). PubMed: 32293494
- MacFarlane EG et al. Lineage-specific events underlie aortic root aneurysm pathogenesis in Loeys-Dietz syndrome. J Clin Invest 129:659-675 (2019). PubMed: 30614814
- He W et al. Synergistic effects of recombinant Lentiviral-mediated BMP2 and TGF-beta3 on the osteogenic differentiation of rat bone marrow mesenchymal stem cells in vitro. Cytokine 120:1-8 (2019). PubMed: 30991228
- Yang J et al. Transforming growth factor-ß is involved in maintaining oocyte meiotic arrest by promoting natriuretic peptide type C expression in mouse granulosa cells. Cell Death Dis 10:558 (2019). PubMed: 31332164