Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab15537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use at an assay dependent concentration.

The immunogen for ab15537 is located close to C-terminus and therefore should be able to detect the proform at 50 kDa and mature form at 12.5 kDa under reducing condition.

IHC-P 1/100. Incubate for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. Ab15537 demonstrates cytoplasmic staining.
IP Use at an assay dependent concentration.

Target

  • Function
    Involved in embryogenesis and cell differentiation.
  • Involvement in disease
    Defects in TGFB3 are a cause of familial arrhythmogenic right ventricular dysplasia type 1 (ARVD1) [MIM:107970]; also known as arrhythmogenic right ventricular cardiomyopathy 1 (ARVC1). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARVD antibody
    • ARVD1 antibody
    • FLJ16571 antibody
    • LDS5 antibody
    • MGC105479 antibody
    • MGC118722 antibody
    • prepro-transforming growth factor beta-3 antibody
    • RNHF antibody
    • TGF beta 3 antibody
    • TGF beta3 antibody
    • TGF-beta-3 antibody
    • TGFB 3 antibody
    • Tgfb3 antibody
    • TGFB3_HUMAN antibody
    • transforming growth factor beta 3 antibody
    • Transforming growth factor beta-3 antibody
    see all

Images

  • All lanes : Anti-TGF beta 3 antibody (ab15537) at 1/200 dilution

    Lane 1 : MCF7 (Hu) whole cell lysate
    Lane 2 : U2OS (Hu) whole cell lysate
    Lane 3 : 293T (Hu) whole cell lysate
    Lane 4 : Cos7 (AGMk) whole cell lysate
    Lane 5 : CHO (Hm) whole cell lysate
    Lane 6 : PC12 (Rat) whole cell lysate
    Lane 7 : 3T3 (Ms) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP conjugated swine anti-rabbit antibody at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 50 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 30 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 6 minutes


    The blot was blocked with 5% milk for 1 hour at 21°C and incubated with the primary antibody for 12 hours at 4°C.

    Detects a 50 kDa species, which is the presumed TGF-Beta 3 precursor, across all cell lysates and species tested including: Hu, AGMk Ms, Rat, and Hm. Higher molecular weight species are observed in all lysates, which could be glycosylated forms of the precursor. A fast migrating species (~14 kDa) was observed in U2OS cell lysate (Lane 2), which could be the cleaved, active form of TGF-Beta3.

    See Abreview

  • Human placenta stained with ab15537 by Immunohistochemistry (FFPE-sections).
  • ab15537 at 1/200 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before incubation with the antibody for 30 minutes. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • ICC/IF image of ab15537 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15537 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Li M  et al. Genetically-modified bone mesenchymal stem cells with TGF-ß3 improve wound healing and reduce scar tissue formation in a rabbit model. Exp Cell Res 367:24-29 (2018). WB . Read more (PubMed: 29453974) »
  • Nicholas SE  et al. Unravelling the interplay of sphingolipids and TGF-ß signaling in the human corneal stroma. PLoS One 12:e0182390 (2017). WB . Read more (PubMed: 28806736) »
See all 17 Publications for this product

Customer reviews and Q&As

1-10 of 15 Abreviews or Q&A

Question
Answer


We do not have any experimental data regarding the cross-reactivity of ab15537 with TGF beta 1 and 2. We compared the immunogen sequence for ab15537 with that of the sequences for TGF beta 1 and 2 (P01137 / P61812 - Uniprot) and the percentage homology is 57% and 76%, respectively. Thus it is unlikely that ab15537 will cross react with TGF beta 1 and 2.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
s-block (Roche) as blocking agent for 4 minute(s) · Concentration: 000000% · Temperature: RT°C
Antigen retrieval step
None
Sample
Cow Tissue sections (Placentome)
Specification
Placentome
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 01 2014

Answer

Thank you for your email.

There may be some antibodies where I might have suggested the human tissues or cells. I apologize for this. I have included the positive control information irrespective of species so similar mouse tissues sections can used as positive control.

Actually the positive control information for each antibody is present on the datasheet; please check the datasheet. Alternatively, we always follow human protein Atlas database (http://www.proteinatlas.org/index.php). The second option is, searching positive control for other antibodies in catalogue against same target; the cell line or tissue sections tested with those antibodies could also be used as positive control. The third place to search for is literature.

I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

Read More

Answer

Thank you for contacting us.

The positive control for ab36392 is IL2 activatedcultured NK cells.

ab9918; human tonsil sections, human cervical squamous cell carcinoma section

ab34674; human artery or colon tissue sections

ab15537; HepG2 cells. mouse brain sections, human placenta sections; Human hepatocellular liver carcinoma cell line; Human hepatocarcinoma tissue sections

ab2557; mouse spleen tissue sections

ab9475; human tonsil tissue sections

ab22378; mouse spleen tissue sections

ab51312; Thymus tissue sections, human pancreas tissue sections.

The best database to search for positive control is Human Protein Atlas. Please click the link http://www.proteinatlas.org/index.php

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse retina whole cell lysate)
Loading amount
50 µg
Specification
mouse retina whole cell lysate
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 22 2011

Answer

Thank you for your patience. I would like to forward some further information to your customer. We do not have any data regarding the cross-reactivity of TGF beta 3 with either TGF beta 1 or beta 2. I compared the immunogen sequence with that of TGFb1 (P01137) and TGFb2 (P61812) and the percentage homology is 57% and 76%, respectively. Theoretically ab15537 will not cross-react with TGFb1 but it may cross-react with TGFb2. Please do not hesitate to contact me with additional questions or concerns.

Read More

Answer

Thank you for your enquiry. Unfortunately, the immunogen sequence of ab15537 is proprietary and can not be released. However, I can confirm that the sequence is 100% identical to the human recombinant TGF beta 3 sequence provided. I hope this information will be helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T)
Total protein in input
250 µg
Specification
293T
Immuno-precipitation step
Protein A

Abcam user community

Verified customer

Submitted Dec 03 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Anal cancer)
Specification
Anal cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 02 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7, U2OS and 293T cell lines)
Loading amount
30 µg
Specification
MCF7, U2OS and 293T cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 02 2007

1-10 of 15 Abreviews or Q&A

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