Overview

  • Product name

  • Description

    Rabbit polyclonal to TGF beta 3
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of total TGF ß3 protein.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from human TGF ß3.

  • Positive control

    • Extracts from COS7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab53727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 47 kDa.
ELISA 1/10000.

Target

  • Function

    Involved in embryogenesis and cell differentiation.
  • Involvement in disease

    Defects in TGFB3 are a cause of familial arrhythmogenic right ventricular dysplasia type 1 (ARVD1) [MIM:107970]; also known as arrhythmogenic right ventricular cardiomyopathy 1 (ARVC1). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
  • Sequence similarities

    Belongs to the TGF-beta family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARVD antibody
    • ARVD1 antibody
    • FLJ16571 antibody
    • LDS5 antibody
    • MGC105479 antibody
    • MGC118722 antibody
    • prepro-transforming growth factor beta-3 antibody
    • RNHF antibody
    • TGF beta 3 antibody
    • TGF beta3 antibody
    • TGF-beta-3 antibody
    • TGFB 3 antibody
    • Tgfb3 antibody
    • TGFB3_HUMAN antibody
    • transforming growth factor beta 3 antibody
    • Transforming growth factor beta-3 antibody
    see all

Images

  • All lanes : Anti-TGF beta 3 antibody (ab53727) at 1/500 dilution

    Lane 1 : extracts from COS7 cells
    Lane 2 : extracts from COS7 cells with immunizing peptide

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 11 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 65 kDa (possible non-specific binding)



    The active molecule is a nonglycosylated, homodimer comprised of 13-kDa polypeptide chains linked by disulphide bonds.

References

ab53727 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry.

ab9758 - the major band at44.3 kDa corresponding to the unprocessed (pre-pro-TGF beta 1) form, the mature processed TGF beta 1 is a 25 kD homodimer composed of two 12.5 kDa subunits joined by disulfide bonds. As the Western blot image indicates a 25 kDa band is seen in the non-reducing lane and a 12 kDa band in the reducing lane. Please visit UniProt website for further information at: http://www.uniprot.org/uniprot/P01137

ab36495 - the major band at 47.8 kDa corresponding to the unprocessed form;the mature (processed)can form homodimers. UniProt: http://www.uniprot.org/uniprot/P61812

ab53727 - the major band at 47.3 kDacorresponding to the unprocessed form, the mature (processed) can form homodimers. UniProt:http://www.uniprot.org/uniprot/P10600

I hope this helps and if I can assist further, please do not hesitate to contact me.

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Answer

The immunogen is 10aa long. The cross-reactivity against TGFb1 and TGFb2 has not been determined. However, the homology with the immunogen is 80% for both sequences. Thus, cross-reactivity could be possible.

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