Anti-TGF beta Receptor I antibody (ab121024)
Key features and details
- Goat polyclonal to TGF beta Receptor I
- Suitable for: IHC-P, Flow Cyt (Intra), WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TGF beta Receptor I antibody
See all TGF beta Receptor I primary antibodies -
Description
Goat polyclonal to TGF beta Receptor I -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Rat
-
Immunogen
Synthetic peptide within Human TGF beta Receptor I aa 50-150 (internal sequence). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Corresponds to NP_004603.1 and NP_001124388.1.
Database link: P36897 -
Positive control
- WB: Rat liver and lung tissue lysates. IHC-P: Human adrenal gland and small intestine tissues. Flow Cyt (Intra): HeLa cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab121024 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| WB |
Rat
|
| All applications |
Mouse
Rabbit
Horse
Cow
Dog
Pig
Rhesus monkey
Gorilla
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
|
|
| WB | (1) |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
3% milk in TBST for blocking, 1 hr at RT. Primary antibody diluted in blocking buffer, 1 hr at room temperature. |
| Notes |
|---|
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). 3% milk in TBST for blocking, 1 hr at RT. Primary antibody diluted in blocking buffer, 1 hr at room temperature. |
Target
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Function
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for TGF-beta. -
Tissue specificity
Found in all tissues examined, most abundant in placenta and least abundant in brain and heart. -
Involvement in disease
Defects in TGFBR1 are the cause of Loeys-Dietz syndrome type 1A (LDS1A) [MIM:609192]; also known as Furlong syndrome or Loeys-Dietz aortic aneurysm syndrome (LDAS). LDS1 is an aortic aneurysm syndrome with widespread systemic involvement. The disorder is characterized by arterial tortuosity and aneurysms, craniosynostosis, hypertelorism, and bifid uvula or cleft palate. Other findings include exotropy, micrognathia and retrognathia, structural brain abnormalities, intellectual deficit, congenital heart disease, translucent skin, joint hyperlaxity and aneurysm with dissection throughout the arterial tree.
Defects in TGFBR1 are the cause of Loeys-Dietz syndrome type 2A (LDS2A) [MIM:608967]. LDS2 is an aortic aneurysm syndrome with widespread systemic involvement. Physical findings include prominent joint laxity, easy bruising, wide and atrophic scars, velvety and translucent skin with easily visible veins, spontaneous rupture of the spleen or bowel, diffuse arterial aneurysms and dissections, and catastrophic complications of pregnancy, including rupture of the gravid uterus and the arteries, either during pregnancy or in the immediate postpartum period. LDS2 is characterized by the absence of craniofacial abnormalities with the exception of bifid uvula that can be present in some patients.
Defects in TGFBR1 are the cause of aortic aneurysm familial thoracic type 5 (AAT5) [MIM:608967]. Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated at basal levels in the absence of ligand binding. Activated by multiple phosphorylation, mainly in the GS region. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 282382 Cow
- Entrez Gene: 7046 Human
- Entrez Gene: 21812 Mouse
- Entrez Gene: 396665 Pig
- Omim: 190181 Human
- SwissProt: O46680 Cow
- SwissProt: P36897 Human
- SwissProt: Q64729 Mouse
see all -
Alternative names
- AAT 5 antibody
- AAT5 antibody
- Activin A receptor type II like kinase 53kDa antibody
see all
Images
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Western blot - Anti-TGF beta Receptor I antibody (ab121024)Lane 1 : Anti-TGF beta Receptor I antibody (ab121024) at 0.1 µg/ml
Lane 2 : Anti-TGF beta Receptor I antibody (ab121024) at 0.03 µg/ml
Lane 1 : Rat liver lysate in RIPA buffer
Lane 2 : Rat lung lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 55 kDa
Observed band size: 55 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
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Flow Cytometry (Intracellular) - Anti-TGF beta Receptor I antibody (ab121024)Flow cytometric (Intracellular) analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Datasheets and documents
References (4)
ab121024 has been referenced in 4 publications.
- Sun H et al. Long Non-Coding RNA (LncRNA)-ATB Promotes Inflammation, Cell Apoptosis and Senescence in Transforming Growth Factor-ß1 (TGF-ß1) Induced Human Kidney 2 (HK-2) Cells via TGFß/SMAD2/3 Signaling Pathway. Med Sci Monit 26:e922029 (2020). PubMed: 32447340
- Lou LL et al. (+)-Isobicyclogermacrenal and spathulenol from Aristolochia yunnanensis alleviate cardiac fibrosis by inhibiting transforming growth factor ß/small mother against decapentaplegic signaling pathway. Phytother Res 33:214-223 (2019). PubMed: 30375049
- Seki M et al. Periostin-expressing cell-specific transforming growth factor-ß inhibition in pulmonary artery prevents pulmonary arterial hypertension. PLoS One 14:e0220795 (2019). PubMed: 31437169
- Yuan M et al. Proteomic Analysis of Nucleus Pulposus Cell-derived Extracellular Matrix Niche and Its Effect on Phenotypic Alteration of Dermal Fibroblasts. Sci Rep 8:1512 (2018). IHC-P ; Rabbit . PubMed: 29367647
