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    tgf-beta-receptor-i-antibody-ab121024.pdf

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Cardiovascular Angiogenesis Growth Factors TGF
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Anti-TGF beta Receptor I antibody (ab121024)

  • Datasheet
Reviews (1) Submit a question References (4)

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Western blot - Anti-TGF beta Receptor I antibody (ab121024)
  • Flow Cytometry (Intracellular) - Anti-TGF beta Receptor I antibody (ab121024)

Key features and details

  • Goat polyclonal to TGF beta Receptor I
  • Suitable for: IHC-P, Flow Cyt (Intra), WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant human TGF beta Receptor I protein (ab105908)
Secondary
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Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
Primary
Product image
Anti-TGF beta Receptor II antibody [EPR14673] (ab184948)

View more associated products

Overview

  • Product name

    Anti-TGF beta Receptor I antibody
    See all TGF beta Receptor I primary antibodies
  • Description

    Goat polyclonal to TGF beta Receptor I
  • Host species

    Goat
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human TGF beta Receptor I aa 50-150 (internal sequence). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Corresponds to NP_004603.1 and NP_001124388.1.
    Database link: P36897

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Rat liver and lung tissue lysates. IHC-P: Human adrenal gland and small intestine tissues. Flow Cyt (Intra): HeLa cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab121024 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • TGF
    • Signal Transduction
    • Growth Factors/Hormones
    • TGF
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Surface Molecules
    • Cancer
    • Growth factors
    • TGF
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant human TGF beta Receptor I protein (ab105908)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab121024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Rat
All applications
Mouse
Rabbit
Horse
Cow
Dog
Pig
Rhesus monkey
Gorilla
Application Abreviews Notes
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)
Use a concentration of 10 µg/ml.
WB (1)
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

3% milk in TBST for blocking, 1 hr at RT. Primary antibody diluted in blocking buffer, 1 hr at room temperature.

Notes
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)
Use a concentration of 10 µg/ml.
WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

3% milk in TBST for blocking, 1 hr at RT. Primary antibody diluted in blocking buffer, 1 hr at room temperature.

Target

  • Function

    On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for TGF-beta.
  • Tissue specificity

    Found in all tissues examined, most abundant in placenta and least abundant in brain and heart.
  • Involvement in disease

    Defects in TGFBR1 are the cause of Loeys-Dietz syndrome type 1A (LDS1A) [MIM:609192]; also known as Furlong syndrome or Loeys-Dietz aortic aneurysm syndrome (LDAS). LDS1 is an aortic aneurysm syndrome with widespread systemic involvement. The disorder is characterized by arterial tortuosity and aneurysms, craniosynostosis, hypertelorism, and bifid uvula or cleft palate. Other findings include exotropy, micrognathia and retrognathia, structural brain abnormalities, intellectual deficit, congenital heart disease, translucent skin, joint hyperlaxity and aneurysm with dissection throughout the arterial tree.
    Defects in TGFBR1 are the cause of Loeys-Dietz syndrome type 2A (LDS2A) [MIM:608967]. LDS2 is an aortic aneurysm syndrome with widespread systemic involvement. Physical findings include prominent joint laxity, easy bruising, wide and atrophic scars, velvety and translucent skin with easily visible veins, spontaneous rupture of the spleen or bowel, diffuse arterial aneurysms and dissections, and catastrophic complications of pregnancy, including rupture of the gravid uterus and the arteries, either during pregnancy or in the immediate postpartum period. LDS2 is characterized by the absence of craniofacial abnormalities with the exception of bifid uvula that can be present in some patients.
    Defects in TGFBR1 are the cause of aortic aneurysm familial thoracic type 5 (AAT5) [MIM:608967]. Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 GS domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated at basal levels in the absence of ligand binding. Activated by multiple phosphorylation, mainly in the GS region.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P36897 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 282382 Cow
    • Entrez Gene: 7046 Human
    • Entrez Gene: 21812 Mouse
    • Entrez Gene: 396665 Pig
    • Omim: 190181 Human
    • SwissProt: O46680 Cow
    • SwissProt: P36897 Human
    • SwissProt: Q64729 Mouse
    • SwissProt: Q5CD18 Pig
    • Unigene: 494622 Human
    • Unigene: 197552 Mouse
    see all
  • Alternative names

    • AAT 5 antibody
    • AAT5 antibody
    • Activin A receptor type II like kinase 53kDa antibody
    • Activin A receptor type II like kinase antibody
    • Activin A receptor type II like kinase, 53kD antibody
    • Activin A receptor type II like protein kinase of 53kD antibody
    • activin A receptor type II-like kinase, 53kDa antibody
    • activin A receptor type II-like protein kinase of 53kD antibody
    • Activin receptor like kinase 5 antibody
    • Activin receptor-like kinase 5 antibody
    • ACVRLK 4 antibody
    • ACVRLK4 antibody
    • ALK 5 antibody
    • ALK-5 antibody
    • ALK5 antibody
    • LDS1A antibody
    • LDS2A antibody
    • MSSE antibody
    • Serine/threonine protein kinase receptor R4 antibody
    • Serine/threonine-protein kinase receptor R4 antibody
    • SKR 4 antibody
    • SKR4 antibody
    • TbetaR I antibody
    • TbetaR-I antibody
    • TGF beta receptor type 1 antibody
    • TGF beta receptor type I antibody
    • TGF beta type I receptor antibody
    • TGF-beta receptor type I antibody
    • TGF-beta receptor type-1 antibody
    • TGF-beta type I receptor antibody
    • TGFBR 1 antibody
    • TGFBR1 antibody
    • TGFBR1 protein antibody
    • TGFR 1 antibody
    • TGFR-1 antibody
    • TGFR1 antibody
    • TGFR1_HUMAN antibody
    • Transforming growth factor beta receptor 1 antibody
    • Transforming growth factor beta receptor I (activin A receptor type II like kinase, 53kD) antibody
    • Transforming growth factor beta receptor I antibody
    • transforming growth factor, beta receptor 1 antibody
    • transforming growth factor, beta receptor I (activin A receptor type II-like kinase, 53kD) antibody
    • Transforming growth factor-beta receptor type I antibody
    see all

Images

  • Western blot - Anti-TGF beta Receptor I antibody (ab121024)
    Western blot - Anti-TGF beta Receptor I antibody (ab121024)
    Lane 1 : Anti-TGF beta Receptor I antibody (ab121024) at 0.1 µg/ml
    Lane 2 : Anti-TGF beta Receptor I antibody (ab121024) at 0.03 µg/ml

    Lane 1 : Rat liver lysate in RIPA buffer
    Lane 2 : Rat lung lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Primary incubation 1 hour at room temperature. Detected by chemiluminescence.

  • Flow Cytometry (Intracellular) - Anti-TGF beta Receptor I antibody (ab121024)
    Flow Cytometry (Intracellular) - Anti-TGF beta Receptor I antibody (ab121024)

    Flow cytometric (Intracellular) analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (4)

    Publishing research using ab121024? Please let us know so that we can cite the reference in this datasheet.

    ab121024 has been referenced in 4 publications.

    • Sun H  et al. Long Non-Coding RNA (LncRNA)-ATB Promotes Inflammation, Cell Apoptosis and Senescence in Transforming Growth Factor-ß1 (TGF-ß1) Induced Human Kidney 2 (HK-2) Cells via TGFß/SMAD2/3 Signaling Pathway. Med Sci Monit 26:e922029 (2020). PubMed: 32447340
    • Lou LL  et al. (+)-Isobicyclogermacrenal and spathulenol from Aristolochia yunnanensis alleviate cardiac fibrosis by inhibiting transforming growth factor ß/small mother against decapentaplegic signaling pathway. Phytother Res 33:214-223 (2019). PubMed: 30375049
    • Seki M  et al. Periostin-expressing cell-specific transforming growth factor-ß inhibition in pulmonary artery prevents pulmonary arterial hypertension. PLoS One 14:e0220795 (2019). PubMed: 31437169
    • Yuan M  et al. Proteomic Analysis of Nucleus Pulposus Cell-derived Extracellular Matrix Niche and Its Effect on Phenotypic Alteration of Dermal Fibroblasts. Sci Rep 8:1512 (2018). IHC-P ; Rabbit . PubMed: 29367647

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-TGF beta Receptor I antibody

    Poor
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Cancer cell)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Treatment
    transfected with TGFBR1
    Specification
    Cancer cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 36°C
    Read More

    Eun-Jeong Yu

    Verified customer

    Submitted Dec 09 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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