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Cardiovascular Angiogenesis Growth Factors TGF
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Anti-TGF beta Receptor I antibody (ab31013)

  • Datasheet
  • SDS
Reviews (31)Q&A (8)References (133)

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Western blot - Anti-TGF beta Receptor I antibody (ab31013)
  • Western blot - Anti-TGF beta Receptor I antibody (ab31013)
  • Western blot - Anti-TGF beta Receptor I antibody (ab31013)
  • Western blot - Anti-TGF beta Receptor I antibody (ab31013)

Key features and details

  • Rabbit polyclonal to TGF beta Receptor I
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant human TGF beta Receptor I protein (ab105908)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
Human TGFBR1 knockout A549 cell line (ab277894)

View more associated products

Overview

  • Product name

    Anti-TGF beta Receptor I antibody
    See all TGF beta Receptor I primary antibodies
  • Description

    Rabbit polyclonal to TGF beta Receptor I
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human TGF beta Receptor I aa 150-250. The exact sequence is proprietary.
    Database link: P36897

  • Positive control

    • Purchase matching WB positive control:Recombinant human TGF beta Receptor I protein
  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.



    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • TGF
    • Signal Transduction
    • Growth Factors/Hormones
    • TGF
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Surface Molecules
    • Cancer
    • Growth factors
    • TGF
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant human TGF beta Receptor I protein (ab105908)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab31013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Rat
Horse
Chicken
Cow
Pig
Xenopus laevis
Application Abreviews Notes
WB (14)
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.

Target

  • Function

    On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for TGF-beta.
  • Tissue specificity

    Found in all tissues examined, most abundant in placenta and least abundant in brain and heart.
  • Involvement in disease

    Defects in TGFBR1 are the cause of Loeys-Dietz syndrome type 1A (LDS1A) [MIM:609192]; also known as Furlong syndrome or Loeys-Dietz aortic aneurysm syndrome (LDAS). LDS1 is an aortic aneurysm syndrome with widespread systemic involvement. The disorder is characterized by arterial tortuosity and aneurysms, craniosynostosis, hypertelorism, and bifid uvula or cleft palate. Other findings include exotropy, micrognathia and retrognathia, structural brain abnormalities, intellectual deficit, congenital heart disease, translucent skin, joint hyperlaxity and aneurysm with dissection throughout the arterial tree.
    Defects in TGFBR1 are the cause of Loeys-Dietz syndrome type 2A (LDS2A) [MIM:608967]. LDS2 is an aortic aneurysm syndrome with widespread systemic involvement. Physical findings include prominent joint laxity, easy bruising, wide and atrophic scars, velvety and translucent skin with easily visible veins, spontaneous rupture of the spleen or bowel, diffuse arterial aneurysms and dissections, and catastrophic complications of pregnancy, including rupture of the gravid uterus and the arteries, either during pregnancy or in the immediate postpartum period. LDS2 is characterized by the absence of craniofacial abnormalities with the exception of bifid uvula that can be present in some patients.
    Defects in TGFBR1 are the cause of aortic aneurysm familial thoracic type 5 (AAT5) [MIM:608967]. Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 GS domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated at basal levels in the absence of ligand binding. Activated by multiple phosphorylation, mainly in the GS region.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P36897 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 282382 Cow
    • Entrez Gene: 7046 Human
    • Entrez Gene: 21812 Mouse
    • Entrez Gene: 396665 Pig
    • Entrez Gene: 29591 Rat
    • Omim: 190181 Human
    • SwissProt: O46680 Cow
    • SwissProt: P36897 Human
    • SwissProt: Q64729 Mouse
    • SwissProt: Q5CD18 Pig
    • SwissProt: P80204 Rat
    • Unigene: 494622 Human
    • Unigene: 197552 Mouse
    • Unigene: 44402 Rat
    see all
  • Alternative names

    • AAT 5 antibody
    • AAT5 antibody
    • Activin A receptor type II like kinase 53kDa antibody
    • Activin A receptor type II like kinase antibody
    • Activin A receptor type II like kinase, 53kD antibody
    • Activin A receptor type II like protein kinase of 53kD antibody
    • activin A receptor type II-like kinase, 53kDa antibody
    • activin A receptor type II-like protein kinase of 53kD antibody
    • Activin receptor like kinase 5 antibody
    • Activin receptor-like kinase 5 antibody
    • ACVRLK 4 antibody
    • ACVRLK4 antibody
    • ALK 5 antibody
    • ALK-5 antibody
    • ALK5 antibody
    • LDS1A antibody
    • LDS2A antibody
    • MSSE antibody
    • Serine/threonine protein kinase receptor R4 antibody
    • Serine/threonine-protein kinase receptor R4 antibody
    • SKR 4 antibody
    • SKR4 antibody
    • TbetaR I antibody
    • TbetaR-I antibody
    • TGF beta receptor type 1 antibody
    • TGF beta receptor type I antibody
    • TGF beta type I receptor antibody
    • TGF-beta receptor type I antibody
    • TGF-beta receptor type-1 antibody
    • TGF-beta type I receptor antibody
    • TGFBR 1 antibody
    • TGFBR1 antibody
    • TGFBR1 protein antibody
    • TGFR 1 antibody
    • TGFR-1 antibody
    • TGFR1 antibody
    • TGFR1_HUMAN antibody
    • Transforming growth factor beta receptor 1 antibody
    • Transforming growth factor beta receptor I (activin A receptor type II like kinase, 53kD) antibody
    • Transforming growth factor beta receptor I antibody
    • transforming growth factor, beta receptor 1 antibody
    • transforming growth factor, beta receptor I (activin A receptor type II-like kinase, 53kD) antibody
    • Transforming growth factor-beta receptor type I antibody
    see all

Images

  • Western blot - Anti-TGF beta Receptor I antibody (ab31013)
    Western blot - Anti-TGF beta Receptor I antibody (ab31013)Verified customer
    All lanes :

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with Anti-TGF beta Receptor I antibody (ab31013)
    Lane 2 : A2780 (Human ovarian cancer cell line) whole cell lysate with Anti-TGF beta Receptor I antibody (ab31013)

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 1/1000 dilution per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa


    Exposure time: 15 seconds


    Primary antibody incubated for 15 hours at 4°C in TBST.

    3% BSA used as blocking agent for 1 hour at 23°C.

    See Abreview

  • Western blot - Anti-TGF beta Receptor I antibody (ab31013)
    Western blot - Anti-TGF beta Receptor I antibody (ab31013)This image is courtesy of an anonymous Abreview
    Anti-TGF beta Receptor I antibody (ab31013) at 1/1000 dilution (for 18 hours at 4°C) + Mouse intestine whole tissue lysate at 15 µg

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/8000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa


    Exposure time: 1 minute


    Blocking Step: 5% milk for 1 hour at 4°C

    See Abreview

  • Western blot - Anti-TGF beta Receptor I antibody (ab31013)
    Western blot - Anti-TGF beta Receptor I antibody (ab31013)
    Anti-TGF beta Receptor I antibody (ab31013) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) whole cell lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa

  • Western blot - Anti-TGF beta Receptor I antibody (ab31013)
    Western blot - Anti-TGF beta Receptor I antibody (ab31013)This image is courtesy of an anonymous Abreview
    Anti-TGF beta Receptor I antibody (ab31013) at 1/750 dilution (for 16 hours at 4°C) + Rat intestine whole tissue lysate at 20 µg

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa


    Exposure time: 2 minutes


    Blocking Step: 5% milk for 1 hour at 20°C

    See Abreview

Protocols

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (133)

Publishing research using ab31013? Please let us know so that we can cite the reference in this datasheet.

ab31013 has been referenced in 133 publications.

  • Lai CC  et al. Vitamin D Attenuates Loss of Endothelial Biomarker Expression in Cardio-Endothelial Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32235811
  • Huang Y  et al. Overexpression of miR-133a-3p inhibits fibrosis and proliferation of keloid fibroblasts by regulating IRF5 to inhibit the TGF-ß/Smad2 pathway. Mol Cell Probes N/A:101563 (2020). PubMed: 32205184
  • Lezmi G  et al. Congenital cystic adenomatoid malformations of the lung: an epithelial transcriptomic approach. Respir Res 21:43 (2020). PubMed: 32019538
  • Gao S  et al. Nucleolar and Spindle Associated Protein 1 (NUSAP1) Promotes Bladder Cancer Progression Through the TGF-ß Signaling Pathway. Onco Targets Ther 13:813-825 (2020). PubMed: 32099387
  • Romano GL  et al. Retinal biomarkers and pharmacological targets for Hermansky-Pudlak syndrome 7. Sci Rep 10:3972 (2020). PubMed: 32132582
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review

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1-10 of 31 Abreviews

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TGF beta Receptor I antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Spine)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: hyaluronidase
Permeabilization
No
Specification
Spine
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
zinc buffered formallin
Read More

Abcam user community

Verified customer

Submitted May 17 2018

Western blot abreview for Anti-TGF beta Receptor I antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Cancer Cell lines)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Cancer Cell lines
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Read More

Abcam user community

Verified customer

Submitted May 03 2018

Immunoprecipitation abreview for Anti-TGF beta Receptor I antibody

Poor
Abreviews
Abreviews
Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (bone marrow cells)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
bone marrow cells
Read More

Jing Fu

Verified customer

Submitted May 03 2017

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-TGF beta Receptor I antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.2% Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 10°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Apr 15 2015

Western blot abreview for Anti-TGF beta Receptor I antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (482N1 murine lung cancer cells)
Loading amount
25000 cells
Specification
482N1 murine lung cancer cells
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Dec 03 2012

Western blot abreview for Anti-TGF beta Receptor I antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Pig Tissue lysate - whole (liver)
Loading amount
50 µg
Specification
liver
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Sep 26 2012

Immunoprecipitation abreview for Anti-TGF beta Receptor I antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (Mouse mammary gland, MEFs)
Total protein in input
1000 µg
Specification
Mouse mammary gland, MEFs
Treatment
2ng/ml TGF-b1 for 30min or 5uM SB431542 for 1hr
Immuno-precipitation step
Protein G
Read More

Abcam user community

Verified customer

Submitted Mar 16 2012

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TGF beta Receptor I antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lymph node)
Specification
Lymph node
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted May 31 2011

Immunocytochemistry/ Immunofluorescence abreview for Anti-TGF beta Receptor I antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (liver immortalized cells)
Specification
liver immortalized cells
Fixative
Methanol
Permeabilization
No
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Mar 16 2011

Flow Cytometry abreview for Anti-TGF beta Receptor I antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (liver immortalized cells)
Specification
liver immortalized cells
Preparation
Cell harvesting/tissue preparation method: cell culture
Sample buffer: 5% Mouse Serum + PBS
Fixation
No fixative
Permeabilization
No
Gating Strategy
Negative control without antibody
Read More

Ms. Megha Rajaram

Verified customer

Submitted Mar 08 2011

1-10 of 31 Abreviews

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