Anti-TGF beta Receptor I (phospho S165) antibody (ab112095)
Key features and details
- Rabbit polyclonal to TGF beta Receptor I (phospho S165)
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TGF beta Receptor I (phospho S165) antibody
See all TGF beta Receptor I primary antibodies -
Description
Rabbit polyclonal to TGF beta Receptor I (phospho S165) -
Host species
Rabbit -
Specificity
ab112095 detects endogenous levels of TGF beta Receptor I only when phosphorylated at serine 165. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
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Positive control
- HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.88% Sodium chloride, 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab112095 is affinity-purified from rabbit antiserum by affinity-chromatography using an epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/500.
|
| Notes |
|---|
|
ICC/IF
1/100 - 1/500. |
Target
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Function
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for TGF-beta. -
Tissue specificity
Found in all tissues examined, most abundant in placenta and least abundant in brain and heart. -
Involvement in disease
Defects in TGFBR1 are the cause of Loeys-Dietz syndrome type 1A (LDS1A) [MIM:609192]; also known as Furlong syndrome or Loeys-Dietz aortic aneurysm syndrome (LDAS). LDS1 is an aortic aneurysm syndrome with widespread systemic involvement. The disorder is characterized by arterial tortuosity and aneurysms, craniosynostosis, hypertelorism, and bifid uvula or cleft palate. Other findings include exotropy, micrognathia and retrognathia, structural brain abnormalities, intellectual deficit, congenital heart disease, translucent skin, joint hyperlaxity and aneurysm with dissection throughout the arterial tree.
Defects in TGFBR1 are the cause of Loeys-Dietz syndrome type 2A (LDS2A) [MIM:608967]. LDS2 is an aortic aneurysm syndrome with widespread systemic involvement. Physical findings include prominent joint laxity, easy bruising, wide and atrophic scars, velvety and translucent skin with easily visible veins, spontaneous rupture of the spleen or bowel, diffuse arterial aneurysms and dissections, and catastrophic complications of pregnancy, including rupture of the gravid uterus and the arteries, either during pregnancy or in the immediate postpartum period. LDS2 is characterized by the absence of craniofacial abnormalities with the exception of bifid uvula that can be present in some patients.
Defects in TGFBR1 are the cause of aortic aneurysm familial thoracic type 5 (AAT5) [MIM:608967]. Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated at basal levels in the absence of ligand binding. Activated by multiple phosphorylation, mainly in the GS region. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 7046 Human
- Entrez Gene: 21812 Mouse
- Entrez Gene: 29591 Rat
- Omim: 190181 Human
- SwissProt: P36897 Human
- SwissProt: Q64729 Mouse
- SwissProt: P80204 Rat
- Unigene: 494622 Human
see all -
Alternative names
- AAT 5 antibody
- AAT5 antibody
- Activin A receptor type II like kinase 53kDa antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TGF beta Receptor I (phospho S165) antibody (ab112095)ab112095, at a 1/100 dilution, staining TGF beta Receptor I in HeLa cells by Immunofluorescence, in the absence (left) and presence (right) of the phosphopeptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab112095 has been referenced in 8 publications.
- Lou LL et al. (+)-Isobicyclogermacrenal and spathulenol from Aristolochia yunnanensis alleviate cardiac fibrosis by inhibiting transforming growth factor ß/small mother against decapentaplegic signaling pathway. Phytother Res 33:214-223 (2019). PubMed: 30375049
- Wojciech S et al. The orphan GPR50 receptor promotes constitutive TGFß receptor signaling and protects against cancer development. Nat Commun 9:1216 (2018). PubMed: 29572483
- Dalvi P et al. Hyperactive TGF-ß Signaling in Smooth Muscle Cells Exposed to HIV-protein(s) and Cocaine: Role in Pulmonary Vasculopathy. Sci Rep 7:10433 (2017). WB . PubMed: 28874783
- Liu Y et al. Decorin inhibits the proliferation of HepG2 cells by elevating the expression of transforming growth factor-ß receptor II. Exp Ther Med 12:2191-2195 (2016). WB ; Human . PubMed: 27698710
- Jin K et al. Hepatic Premalignant Alterations Triggered by Human Nephrotoxin Aristolochic Acid I in Canines. Cancer Prev Res (Phila) 9:324-34 (2016). PubMed: 26851235
- Su CT et al. Latent transforming growth factor binding protein 4 regulates transforming growth factor beta receptor stability. Hum Mol Genet 24:4024-36 (2015). PubMed: 25882708
- Silva MT et al. Impaired structural and functional regeneration of skeletal muscles from ß2-adrenoceptor knockout mice. Acta Physiol (Oxf) 211:617-33 (2014). PubMed: 24938737
- Rudalska R et al. In vivo RNAi screening identifies a mechanism of sorafenib resistance in liver cancer. Nat Med 20:1138-46 (2014). PubMed: 25216638