Overview

  • Product name

    Anti-TGF beta Receptor III/TGFBR3 antibody
    See all TGF beta Receptor III/TGFBR3 primary antibodies
  • Description

    Goat polyclonal to TGF beta Receptor III/TGFBR3
  • Host species

    Goat
  • Specificity

    ab18885 recognises TGF-beta Receptor III/TGFBR3.

  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human TGF beta Receptor III/TGFBR3 (extracellular).

  • Positive control

    • IHC-P: human placenta FFPE tissue sections
  • General notes

     This product was previously labelled as TGF beta Receptor III

Properties

Applications

Our Abpromise guarantee covers the use of ab18885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 94 kDa.

Target

  • Function

    Binds to TGF-beta. Could be involved in capturing and retaining TGF-beta for presentation to the signaling receptors.
  • Sequence similarities

    Contains 1 ZP domain.
  • Post-translational
    modifications

    Extensively modified by glycosaminoglycan groups (GAG).
  • Cellular localization

    Secreted. Secreted > extracellular space. Cell membrane. Exists both as a membrane-bound form and as soluble form in serum and in the extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta glycan antibody
    • Betaglycan antibody
    • Betaglycan proteoglycan antibody
    • BGCAN antibody
    • TGBR3_HUMAN antibody
    • TGF beta receptor type 3 antibody
    • TGF beta receptor type III antibody
    • TGF-beta receptor type 3 antibody
    • TGF-beta receptor type III antibody
    • TGFB R3 antibody
    • TGFBR 3 antibody
    • TGFBR3 antibody
    • TGFR 3 antibody
    • TGFR-3 antibody
    • TGFR3 antibody
    • Transforming growth factor beta receptor III antibody
    • Transforming growth factor beta receptor III betaglycan 300kDa antibody
    • Transforming growth factor beta receptor type 3 antibody
    see all

Images

  • IHC image of TGF beta Receptor III/TGFBR3 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18885, 5µg/ml, for 15 mins at room temperature. A donkey anti-goat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Rumilla KM  et al. Hyperinsulinemic hypoglycemia with nesidioblastosis: histologic features and growth factor expression. Mod Pathol : (2008). Read more (PubMed: 18931647) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your patience in awaiting our reply. I have run the immunogen sequence to search against opposums and found only Monodelphis domestica has 74% homology with the immunogen, most other types are much below that. Generally we predict reactivity at 85% homology or higher, so it is most likley not your teachnique, but that the antibody does not work in your species. We have only tested this antibody in human, as indicated on the datasheet.

Please let me know if I can be of further assistance.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up