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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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RecombinantRabMAb

Recombinant Anti-TGFBI antibody [EPR12078(B)] (ab170874)

  • Datasheet
Reviews (3) Submit a question References (12)

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Western blot - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
  • Immunocytochemistry/ Immunofluorescence - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
  • Anti-TGFBI antibody [EPR12078(B)] (ab170874)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12078(B)] to TGFBI
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-TGFBI antibody [EPR12078(B)]
    See all TGFBI primary antibodies
  • Description

    Rabbit monoclonal [EPR12078(B)] to TGFBI
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human TGFBI aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: O60784

  • Positive control

    • Human fetal kidney and Human fetal liver lysates, Human colon tissue and Human uterus tissue, Y79 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12078(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen
    • Signal Transduction
    • Growth Factors/Hormones
    • TGF
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Neuroscience
    • Sensory System
    • Visual system
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Other ECM Proteins
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development

Associated products

  • Alternative Versions

    • Anti-TGFBI antibody [EPR12078(B)] - BSA and Azide free (ab240162)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human TGFBI protein (ab51281)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB (1)
1/1000 - 1/5000. Predicted molecular weight: 75 kDa.
ICC/IF
1/50 - 1/100.
IHC-P (1)
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 75 kDa.
ICC/IF
1/50 - 1/100.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation.
    • Tissue specificity

      Highly expressed in the corneal epithelium.
    • Involvement in disease

      Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD) [MIM:121820]; also known as Cogan corneal dystrophy or map-dot-fingerprint type corneal dystrophy. EBMD is a bilateral anterior corneal dystrophy characterized by grayish epithelial fingerprint lines, geographic map-like lines, and dots (or microcysts) on slit-lamp examination. Pathologic studies show abnormal, redundant basement membrane and intraepithelial lacunae filled with cellular debris. Although this disorder usually is not considered to be inherited, families with autosomal dominant inheritance have been identified.
      Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1) [MIM:121900]; also known as corneal dystrophy granular type. Inheritance is autosomal dominant. Corneal dystrophies show progressive opacification of the cornea leading to severe visual handicap.
      Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1) [MIM:122200]. Inheritance is autosomal dominant.
      Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB) [MIM:602082]; also known as corneal dystrophy of Bowman layer type 2 (CDB2).
      Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB) [MIM:608470]; also known as corneal dystrophy of Bowman layer type 1 (CDB1).
      Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A) [MIM:608471]. CDL3A clinically resembles to lattice corneal dystrophy type 3, but differs in that its age of onset is 70 to 90 years. It has an autosomal dominant inheritance pattern.
      Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD) [MIM:607541]. ACD could be considered a variant of granular dystrophy with a significant amyloidogenic tendency. Inheritance is autosomal dominant.
    • Sequence similarities

      Contains 1 EMI domain.
      Contains 4 FAS1 domains.
    • Post-translational
      modifications

      Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix. May be associated both with microfibrils and with the cell surface.
    • Target information above from: UniProt accession Q15582 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7045 Human
      • Entrez Gene: 21810 Mouse
      • Entrez Gene: 116487 Rat
      • Omim: 601692 Human
      • SwissProt: Q15582 Human
      • SwissProt: P82198 Mouse
      • Unigene: 369397 Human
      • Unigene: 14455 Mouse
      • Unigene: 1046 Rat
      see all
    • Alternative names

      • RGD containing collagen associated protein antibody
      • AI181842 antibody
      • AI747162 antibody
      • Beta ig antibody
      • Beta ig h3 antibody
      • Beta ig-h3 antibody
      • BGH3_HUMAN antibody
      • Big h3 antibody
      • BIGH3 antibody
      • CDB1 antibody
      • CDG2 antibody
      • CDGG1 antibody
      • CSD antibody
      • CSD1 antibody
      • CSD2 antibody
      • CSD3 antibody
      • EBMD antibody
      • Kerato epithelin antibody
      • Kerato-epithelin antibody
      • LCD1 antibody
      • MGC150270 antibody
      • RGD CAP antibody
      • RGD-CAP antibody
      • RGD-containing collagen-associated protein antibody
      • TGFBI antibody
      • TGFBI transforming growth factor, beta induced, 68kDa antibody
      • Transforming growth factor beta induced protein ig h3 antibody
      • Transforming growth factor-beta-induced protein ig-h3 antibody
      see all

    Images

    • Western blot - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
      Western blot - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
      All lanes : Anti-TGFBI antibody [EPR12078(B)] (ab170874) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 75 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TGFBI using ab170874 at a 1/100 dilution

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
      Immunocytochemistry/ Immunofluorescence - Anti-TGFBI antibody [EPR12078(B)] (ab170874)

      Immunofluorescent staining of Y79 cells labeling TGFBI using ab170874 at a 1/50 dilution (green) and DAPI nuclear staining (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)

      Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TGFBI using ab170874 at a 1/100 dilution

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-TGFBI antibody [EPR12078(B)] (ab170874)
      Anti-TGFBI antibody [EPR12078(B)] (ab170874)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (12)

    Publishing research using ab170874? Please let us know so that we can cite the reference in this datasheet.

    ab170874 has been referenced in 12 publications.

    • Wang W  et al. Enterovirus A71 capsid protein VP1 increases blood-brain barrier permeability and virus receptor vimentin on the brain endothelial cells. J Neurovirol 26:84-94 (2020). PubMed: 31512144
    • Leng L  et al. Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth. Stem Cell Res Ther 11:415 (2020). PubMed: 32967725
    • Zhu L  et al. Interference of the long noncoding RNA CDKN2B-AS1 upregulates miR-181a-5p/TGFßI axis to restrain the metastasis and promote apoptosis and senescence of cervical cancer cells. Cancer Med 8:1721-1730 (2019). PubMed: 30884187
    • Liu Y  et al. Competitive endogenous RNA is an intrinsic component of EMT regulatory circuits and modulates EMT. Nat Commun 10:1637 (2019). PubMed: 30967542
    • Zhang R  et al. FcgRIII Deficiency and FcgRIIb Defeciency Promote Renal Injury in Diabetic Mice. Biomed Res Int 2019:3514574 (2019). PubMed: 31534958
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-TGFBI antibody [EPR12078(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Mouse brain tumor)
    Permeabilization
    No
    Specification
    Mouse brain tumor
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 13 2020

    Western blot abreview for Anti-TGFBI antibody [EPR12078(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (mouse brain tumor)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    5 µg
    Specification
    mouse brain tumor
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 13 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TGFBI antibody [EPR12078(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 23 2018

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