Recombinant Anti-TGFBI antibody [EPR12078(B)] (ab170874)
![Western blot - Anti-TGFBI antibody [EPR12078(B)] (ab170874)](https://www.abcam.com/ps/products/170/ab170874/Images/ab170874-171047-anti-tgfbi-antibody-epr12078b-western-blot.jpg "Western blot - Anti-TGFBI antibody [EPR12078(B)] (ab170874)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12078(B)] to TGFBI
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-TGFBI antibody [EPR12078(B)]
See all TGFBI primary antibodies -
Description
Rabbit monoclonal [EPR12078(B)] to TGFBI -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human TGFBI aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: O60784 -
Positive control
- Human fetal kidney and Human fetal liver lysates, Human colon tissue and Human uterus tissue, Y79 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12078(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 75 kDa.
|
| ICC/IF |
1/50 - 1/100.
|
|
| IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 75 kDa. |
|
ICC/IF
1/50 - 1/100. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation. -
Tissue specificity
Highly expressed in the corneal epithelium. -
Involvement in disease
Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD) [MIM:121820]; also known as Cogan corneal dystrophy or map-dot-fingerprint type corneal dystrophy. EBMD is a bilateral anterior corneal dystrophy characterized by grayish epithelial fingerprint lines, geographic map-like lines, and dots (or microcysts) on slit-lamp examination. Pathologic studies show abnormal, redundant basement membrane and intraepithelial lacunae filled with cellular debris. Although this disorder usually is not considered to be inherited, families with autosomal dominant inheritance have been identified.
Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1) [MIM:121900]; also known as corneal dystrophy granular type. Inheritance is autosomal dominant. Corneal dystrophies show progressive opacification of the cornea leading to severe visual handicap.
Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1) [MIM:122200]. Inheritance is autosomal dominant.
Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB) [MIM:602082]; also known as corneal dystrophy of Bowman layer type 2 (CDB2).
Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB) [MIM:608470]; also known as corneal dystrophy of Bowman layer type 1 (CDB1).
Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A) [MIM:608471]. CDL3A clinically resembles to lattice corneal dystrophy type 3, but differs in that its age of onset is 70 to 90 years. It has an autosomal dominant inheritance pattern.
Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD) [MIM:607541]. ACD could be considered a variant of granular dystrophy with a significant amyloidogenic tendency. Inheritance is autosomal dominant. -
Sequence similarities
Contains 1 EMI domain.
Contains 4 FAS1 domains. -
Post-translational
modificationsGamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
Cellular localization
Secreted > extracellular space > extracellular matrix. May be associated both with microfibrils and with the cell surface. - Information by UniProt
-
Database links
- Entrez Gene: 7045 Human
- Entrez Gene: 21810 Mouse
- Entrez Gene: 116487 Rat
- Omim: 601692 Human
- SwissProt: Q15582 Human
- SwissProt: P82198 Mouse
- Unigene: 369397 Human
- Unigene: 14455 Mouse
see all -
Alternative names
- RGD containing collagen associated protein antibody
- AI181842 antibody
- AI747162 antibody
see all
Images
-
Western blot - Anti-TGFBI antibody [EPR12078(B)] (ab170874)All lanes : Anti-TGFBI antibody [EPR12078(B)] (ab170874) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 75 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)](https://www.abcam.com/ps/products/170/ab170874/Images/ab170874-171048-anti-tgfbi-antibody-epr12078b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TGFBI using ab170874 at a 1/100 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-TGFBI antibody [EPR12078(B)] (ab170874)](https://www.abcam.com/ps/products/170/ab170874/Images/ab170874-171050-anti-tgfbi-antibody-epr12078b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TGFBI antibody [EPR12078(B)] (ab170874)Immunofluorescent staining of Y79 cells labeling TGFBI using ab170874 at a 1/50 dilution (green) and DAPI nuclear staining (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)](https://www.abcam.com/ps/products/170/ab170874/Images/ab170874-171049-anti-tgfbi-antibody-epr12078b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGFBI antibody [EPR12078(B)] (ab170874)Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TGFBI using ab170874 at a 1/100 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Anti-TGFBI antibody [EPR12078(B)] (ab170874)](https://www.abcam.com/ps/products/170/ab170874/Images/ab170874-6-benefits-of-recombinant-antibodies.png)
Anti-TGFBI antibody [EPR12078(B)] (ab170874)
Protocols
Datasheets and documents
References (12)
ab170874 has been referenced in 12 publications.
- Wang W et al. Enterovirus A71 capsid protein VP1 increases blood-brain barrier permeability and virus receptor vimentin on the brain endothelial cells. J Neurovirol 26:84-94 (2020). PubMed: 31512144
- Leng L et al. Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth. Stem Cell Res Ther 11:415 (2020). PubMed: 32967725
- Zhu L et al. Interference of the long noncoding RNA CDKN2B-AS1 upregulates miR-181a-5p/TGFßI axis to restrain the metastasis and promote apoptosis and senescence of cervical cancer cells. Cancer Med 8:1721-1730 (2019). PubMed: 30884187
- Liu Y et al. Competitive endogenous RNA is an intrinsic component of EMT regulatory circuits and modulates EMT. Nat Commun 10:1637 (2019). PubMed: 30967542
- Zhang R et al. FcgRIII Deficiency and FcgRIIb Defeciency Promote Renal Injury in Diabetic Mice. Biomed Res Int 2019:3514574 (2019). PubMed: 31534958
