• Product name
    Anti-TGFBI antibody [EPR12078(B)]
    See all TGFBI primary antibodies
  • Description
    Rabbit monoclonal [EPR12078(B)] to TGFBI
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TGFBI aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: O60784

  • Positive control
    • Human fetal kidney and Human fetal liver lysates, Human colon tissue and Human uterus tissue, Y79 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab170874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 75 kDa.
ICC/IF 1/50 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation.
    • Tissue specificity
      Highly expressed in the corneal epithelium.
    • Involvement in disease
      Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD) [MIM:121820]; also known as Cogan corneal dystrophy or map-dot-fingerprint type corneal dystrophy. EBMD is a bilateral anterior corneal dystrophy characterized by grayish epithelial fingerprint lines, geographic map-like lines, and dots (or microcysts) on slit-lamp examination. Pathologic studies show abnormal, redundant basement membrane and intraepithelial lacunae filled with cellular debris. Although this disorder usually is not considered to be inherited, families with autosomal dominant inheritance have been identified.
      Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1) [MIM:121900]; also known as corneal dystrophy granular type. Inheritance is autosomal dominant. Corneal dystrophies show progressive opacification of the cornea leading to severe visual handicap.
      Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1) [MIM:122200]. Inheritance is autosomal dominant.
      Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB) [MIM:602082]; also known as corneal dystrophy of Bowman layer type 2 (CDB2).
      Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB) [MIM:608470]; also known as corneal dystrophy of Bowman layer type 1 (CDB1).
      Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A) [MIM:608471]. CDL3A clinically resembles to lattice corneal dystrophy type 3, but differs in that its age of onset is 70 to 90 years. It has an autosomal dominant inheritance pattern.
      Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD) [MIM:607541]. ACD could be considered a variant of granular dystrophy with a significant amyloidogenic tendency. Inheritance is autosomal dominant.
    • Sequence similarities
      Contains 1 EMI domain.
      Contains 4 FAS1 domains.
    • Post-translational
      Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix. May be associated both with microfibrils and with the cell surface.
    • Information by UniProt
    • Database links
    • Alternative names
      • RGD containing collagen associated protein antibody
      • AI181842 antibody
      • AI747162 antibody
      • Beta ig antibody
      • Beta ig h3 antibody
      • Beta ig-h3 antibody
      • BGH3_HUMAN antibody
      • Big h3 antibody
      • BIGH3 antibody
      • CDB1 antibody
      • CDG2 antibody
      • CDGG1 antibody
      • CSD antibody
      • CSD1 antibody
      • CSD2 antibody
      • CSD3 antibody
      • EBMD antibody
      • Kerato epithelin antibody
      • Kerato-epithelin antibody
      • LCD1 antibody
      • MGC150270 antibody
      • RGD CAP antibody
      • RGD-CAP antibody
      • RGD-containing collagen-associated protein antibody
      • TGFBI antibody
      • TGFBI transforming growth factor, beta induced, 68kDa antibody
      • Transforming growth factor beta induced protein ig h3 antibody
      • Transforming growth factor-beta-induced protein ig-h3 antibody
      see all


    • All lanes : Anti-TGFBI antibody [EPR12078(B)] (ab170874) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 75 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TGFBI using ab170874 at a 1/100 dilution

    • Immunofluorescent staining of Y79 cells labeling TGFBI using ab170874 at a 1/50 dilution (green) and DAPI nuclear staining (blue).

    • Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TGFBI using ab170874 at a 1/100 dilution


    This product has been referenced in:
    • Zhang J & Li Z ß-inducible gene-h3 promotes human breast carcinoma cell metastasis by activating the phosphatidylinositol 3-kinase/protein kinase B signaling pathway. Exp Ther Med 15:2910-2916 (2018). Read more (PubMed: 29599830) »
    • Wei Y  et al. Assessment of tumor growth factor-ß1 neutralizing antibody in the treatment of allergic rhinitis and asthma. Exp Ther Med 15:649-656 (2018). Read more (PubMed: 29399067) »
    See all 4 Publications for this product

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Rat Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

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    Verified customer

    Submitted Jan 23 2018


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