Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse brain tumor)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
5 µg
Specification
mouse brain tumor
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Thermo Scientific Pierce ECL Plus Substr
Negative control
lane 1: proneural subtype tumor lysate
Exposure
5 second(s)
Bands
Specific: 60 kDa
Positive control
lane 2: mesenchymal subtype tumor lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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Submitted Jul 13 2020