Recombinant
RabMAb

Anti-TGFBI antibody [EPR12079(B)] (ab169771)

Overview

  • Product name
    Anti-TGFBI antibody [EPR12079(B)]
    See all TGFBI primary antibodies
  • Description
    Rabbit monoclonal [EPR12079(B)] to TGFBI
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human TGFBI (UniProt Q15582).

  • Positive control
    • Human fetal kidney, Human small intestine, Mouse eyeball and Rat small intestine lysate; Human colon adenocarcinoma and uterus tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab169771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500.
WB 1/1000 - 1/5000. Predicted molecular weight: 75 kDa.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function
      Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation.
    • Tissue specificity
      Highly expressed in the corneal epithelium.
    • Involvement in disease
      Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD) [MIM:121820]; also known as Cogan corneal dystrophy or map-dot-fingerprint type corneal dystrophy. EBMD is a bilateral anterior corneal dystrophy characterized by grayish epithelial fingerprint lines, geographic map-like lines, and dots (or microcysts) on slit-lamp examination. Pathologic studies show abnormal, redundant basement membrane and intraepithelial lacunae filled with cellular debris. Although this disorder usually is not considered to be inherited, families with autosomal dominant inheritance have been identified.
      Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1) [MIM:121900]; also known as corneal dystrophy granular type. Inheritance is autosomal dominant. Corneal dystrophies show progressive opacification of the cornea leading to severe visual handicap.
      Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1) [MIM:122200]. Inheritance is autosomal dominant.
      Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB) [MIM:602082]; also known as corneal dystrophy of Bowman layer type 2 (CDB2).
      Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB) [MIM:608470]; also known as corneal dystrophy of Bowman layer type 1 (CDB1).
      Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A) [MIM:608471]. CDL3A clinically resembles to lattice corneal dystrophy type 3, but differs in that its age of onset is 70 to 90 years. It has an autosomal dominant inheritance pattern.
      Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD) [MIM:607541]. ACD could be considered a variant of granular dystrophy with a significant amyloidogenic tendency. Inheritance is autosomal dominant.
    • Sequence similarities
      Contains 1 EMI domain.
      Contains 4 FAS1 domains.
    • Post-translational
      modifications
      Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix. May be associated both with microfibrils and with the cell surface.
    • Information by UniProt
    • Database links
    • Alternative names
      • RGD containing collagen associated protein antibody
      • AI181842 antibody
      • AI747162 antibody
      • Beta ig antibody
      • Beta ig h3 antibody
      • Beta ig-h3 antibody
      • BGH3_HUMAN antibody
      • Big h3 antibody
      • BIGH3 antibody
      • CDB1 antibody
      • CDG2 antibody
      • CDGG1 antibody
      • CSD antibody
      • CSD1 antibody
      • CSD2 antibody
      • CSD3 antibody
      • EBMD antibody
      • Kerato epithelin antibody
      • Kerato-epithelin antibody
      • LCD1 antibody
      • MGC150270 antibody
      • RGD CAP antibody
      • RGD-CAP antibody
      • RGD-containing collagen-associated protein antibody
      • TGFBI antibody
      • TGFBI transforming growth factor, beta induced, 68kDa antibody
      • Transforming growth factor beta induced protein ig h3 antibody
      • Transforming growth factor-beta-induced protein ig-h3 antibody
      see all

    Images

    • All lanes : Anti-TGFBI antibody [EPR12079(B)] (ab169771) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human small intestine lysate
      Lane 3 : Mouse eyeball lysate
      Lane 4 : Rat small intestine lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 75 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling TGFBI with ab169771 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TGFBI with ab169771 at 1/250 dilution.

    References

    This product has been referenced in:
    • Lv J  et al. Renoprotective Effect of the Shen-Yan-Fang-Shuai Formula by Inhibiting TNF-a/NF-?B Signaling Pathway in Diabetic Rats. J Diabetes Res 2017:4319057 (2017). WB, IHC . Read more (PubMed: 28713834) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (10% PAGE)
    Sample
    Mouse Cell lysate - whole cell (Cardiomyocytes)
    Specification
    Cardiomyocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 16 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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