Product nameAnti-TGFBI antibody [EPR12079(B)]
See all TGFBI primary antibodies
DescriptionRabbit monoclonal [EPR12079(B)] to TGFBI
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human TGFBI (UniProt Q15582).
- Human fetal kidney, Human small intestine, Mouse eyeball and Rat small intestine lysate; Human colon adenocarcinoma and uterus tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab169771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/250 - 1/500.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 75 kDa.|
FunctionBinds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation.
Tissue specificityHighly expressed in the corneal epithelium.
Involvement in diseaseDefects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD) [MIM:121820]; also known as Cogan corneal dystrophy or map-dot-fingerprint type corneal dystrophy. EBMD is a bilateral anterior corneal dystrophy characterized by grayish epithelial fingerprint lines, geographic map-like lines, and dots (or microcysts) on slit-lamp examination. Pathologic studies show abnormal, redundant basement membrane and intraepithelial lacunae filled with cellular debris. Although this disorder usually is not considered to be inherited, families with autosomal dominant inheritance have been identified.
Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1) [MIM:121900]; also known as corneal dystrophy granular type. Inheritance is autosomal dominant. Corneal dystrophies show progressive opacification of the cornea leading to severe visual handicap.
Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1) [MIM:122200]. Inheritance is autosomal dominant.
Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB) [MIM:602082]; also known as corneal dystrophy of Bowman layer type 2 (CDB2).
Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB) [MIM:608470]; also known as corneal dystrophy of Bowman layer type 1 (CDB1).
Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A) [MIM:608471]. CDL3A clinically resembles to lattice corneal dystrophy type 3, but differs in that its age of onset is 70 to 90 years. It has an autosomal dominant inheritance pattern.
Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD) [MIM:607541]. ACD could be considered a variant of granular dystrophy with a significant amyloidogenic tendency. Inheritance is autosomal dominant.
Sequence similaritiesContains 1 EMI domain.
Contains 4 FAS1 domains.
modificationsGamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
Cellular localizationSecreted > extracellular space > extracellular matrix. May be associated both with microfibrils and with the cell surface.
- Information by UniProt
- RGD containing collagen associated protein antibody
- AI181842 antibody
- AI747162 antibody
All lanes : Anti-TGFBI antibody [EPR12079(B)] (ab169771) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human small intestine lysate
Lane 3 : Mouse eyeball lysate
Lane 4 : Rat small intestine lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 75 kDa
Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling TGFBI with ab169771 at 1/250 dilution.
Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TGFBI with ab169771 at 1/250 dilution.