Product nameAnti-TGFBI antibody [EPR17990-13]
See all TGFBI primary antibodies
DescriptionRabbit monoclonal [EPR17990-13] to TGFBI
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Rat
Recombinant fragment within Mouse TGFBI aa 500 to the C-terminus. The exact sequence is proprietary.
Database link: P82198
- WB: Rat eyeball and liver lysates; Mouse eyeball and spleen lysates. IP: Mouse eyeball lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab187085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 75 kDa).|
FunctionBinds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation.
Tissue specificityHighly expressed in the corneal epithelium.
Involvement in diseaseDefects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD) [MIM:121820]; also known as Cogan corneal dystrophy or map-dot-fingerprint type corneal dystrophy. EBMD is a bilateral anterior corneal dystrophy characterized by grayish epithelial fingerprint lines, geographic map-like lines, and dots (or microcysts) on slit-lamp examination. Pathologic studies show abnormal, redundant basement membrane and intraepithelial lacunae filled with cellular debris. Although this disorder usually is not considered to be inherited, families with autosomal dominant inheritance have been identified.
Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1) [MIM:121900]; also known as corneal dystrophy granular type. Inheritance is autosomal dominant. Corneal dystrophies show progressive opacification of the cornea leading to severe visual handicap.
Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1) [MIM:122200]. Inheritance is autosomal dominant.
Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB) [MIM:602082]; also known as corneal dystrophy of Bowman layer type 2 (CDB2).
Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB) [MIM:608470]; also known as corneal dystrophy of Bowman layer type 1 (CDB1).
Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A) [MIM:608471]. CDL3A clinically resembles to lattice corneal dystrophy type 3, but differs in that its age of onset is 70 to 90 years. It has an autosomal dominant inheritance pattern.
Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD) [MIM:607541]. ACD could be considered a variant of granular dystrophy with a significant amyloidogenic tendency. Inheritance is autosomal dominant.
Sequence similaritiesContains 1 EMI domain.
Contains 4 FAS1 domains.
modificationsGamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
Cellular localizationSecreted > extracellular space > extracellular matrix. May be associated both with microfibrils and with the cell surface.
- Information by UniProt
- RGD containing collagen associated protein antibody
- AI181842 antibody
- AI747162 antibody
All lanes : Anti-TGFBI antibody [EPR17990-13] (ab187085) at 1/1000 dilution
Lane 1 : Rat eyeball lysate at 20 µg
Lane 2 : Mouse eyeball lysate at 20 µg
Lane 3 : Mouse spleen lysate at 10 µg
Lane 4 : Rat liver lysate at 10 µg
Lanes 1 & 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 75 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Exposure time : Lane 1: 3 minutes; Lane 2: 1 second; Lanes 3 and 4: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular mass observed is consistent with what has been described in the literature (PMID: 19478074).
TGFBI was immunoprecipitated from 0.35 mg of mouse eyeball lysate with ab187085 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187085 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse eyeball lysate 10 µg (Input).
Lane 2: ab187085 IP in mouse eyeball lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187085 in mouse eyeball lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
ab187085 has not yet been referenced specifically in any publications.