Recombinant Anti-TGFBI antibody [EPR17990-13] (ab187085)
![Western blot - Anti-TGFBI antibody [EPR17990-13] (ab187085)](https://www.abcam.com/ps/products/187/ab187085/Images/ab187085-291632-anti-tgfbi-antibody-epr17990-13-western-blot.jpg "Western blot - Anti-TGFBI antibody [EPR17990-13] (ab187085)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17990-13] to TGFBI
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-TGFBI antibody [EPR17990-13]
See all TGFBI primary antibodies -
Description
Rabbit monoclonal [EPR17990-13] to TGFBI -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment within Mouse TGFBI aa 500 to the C-terminus. The exact sequence is proprietary.
Database link: P82198 -
Positive control
- WB: Rat eyeball and liver lysates; Mouse eyeball and spleen lysates. IP: Mouse eyeball lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17990-13 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab187085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 75 kDa). | |
| IP | 1/30. |
Target
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Function
Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation. -
Tissue specificity
Highly expressed in the corneal epithelium. -
Involvement in disease
Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD) [MIM:121820]; also known as Cogan corneal dystrophy or map-dot-fingerprint type corneal dystrophy. EBMD is a bilateral anterior corneal dystrophy characterized by grayish epithelial fingerprint lines, geographic map-like lines, and dots (or microcysts) on slit-lamp examination. Pathologic studies show abnormal, redundant basement membrane and intraepithelial lacunae filled with cellular debris. Although this disorder usually is not considered to be inherited, families with autosomal dominant inheritance have been identified.
Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1) [MIM:121900]; also known as corneal dystrophy granular type. Inheritance is autosomal dominant. Corneal dystrophies show progressive opacification of the cornea leading to severe visual handicap.
Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1) [MIM:122200]. Inheritance is autosomal dominant.
Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB) [MIM:602082]; also known as corneal dystrophy of Bowman layer type 2 (CDB2).
Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB) [MIM:608470]; also known as corneal dystrophy of Bowman layer type 1 (CDB1).
Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A) [MIM:608471]. CDL3A clinically resembles to lattice corneal dystrophy type 3, but differs in that its age of onset is 70 to 90 years. It has an autosomal dominant inheritance pattern.
Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD) [MIM:607541]. ACD could be considered a variant of granular dystrophy with a significant amyloidogenic tendency. Inheritance is autosomal dominant. -
Sequence similarities
Contains 1 EMI domain.
Contains 4 FAS1 domains. -
Post-translational
modificationsGamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
Cellular localization
Secreted > extracellular space > extracellular matrix. May be associated both with microfibrils and with the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 21810 Mouse
- Entrez Gene: 116487 Rat
- SwissProt: P82198 Mouse
- Unigene: 14455 Mouse
- Unigene: 1046 Rat
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Alternative names
- RGD containing collagen associated protein antibody
- AI181842 antibody
- AI747162 antibody
see all
Images
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Western blot - Anti-TGFBI antibody [EPR17990-13] (ab187085)All lanes : Anti-TGFBI antibody [EPR17990-13] (ab187085) at 1/1000 dilution
Lane 1 : Rat eyeball lysate at 20 µg
Lane 2 : Mouse eyeball lysate at 20 µg
Lane 3 : Mouse spleen lysate at 10 µg
Lane 4 : Rat liver lysate at 10 µg
Secondary
Lanes 1 & 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 75 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?Exposure time : Lane 1: 3 minutes; Lane 2: 1 second; Lanes 3 and 4: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular mass observed is consistent with what has been described in the literature (PMID: 19478074).
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![Immunoprecipitation - Anti-TGFBI antibody [EPR17990-13] (ab187085)](https://www.abcam.com/ps/products/187/ab187085/Images/ab187085-291631-anti-tgfbi-antibody-epr17990-13-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TGFBI antibody [EPR17990-13] (ab187085)TGFBI was immunoprecipitated from 0.35 mg of mouse eyeball lysate with ab187085 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187085 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse eyeball lysate 10 µg (Input).
Lane 2: ab187085 IP in mouse eyeball lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187085 in mouse eyeball lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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![Anti-TGFBI antibody [EPR17990-13] (ab187085)](https://www.abcam.com/ps/products/187/ab187085/Images/ab187085-3-benefits-of-recombinant-antibodies.png)
Anti-TGFBI antibody [EPR17990-13] (ab187085)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab187085 has not yet been referenced specifically in any publications.
