Anti-TGG1 / Myrosinase 1 antibody (ab243202)
Key features and details
- Rabbit polyclonal to TGG1 / Myrosinase 1
- Suitable for: WB
- Reacts with: Arabidopsis thaliana
- Isotype: IgG
Overview
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Product name
Anti-TGG1 / Myrosinase 1 antibody -
Description
Rabbit polyclonal to TGG1 / Myrosinase 1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Arabidopsis thaliana -
Immunogen
Synthetic peptide corresponding to Arabidopsis thaliana TGG1/ Myrosinase 1 aa 350-450 conjugated to bovine serum albumin.
Database link: P37702 -
Positive control
- WB: Arabidopsis leaf extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Filter-sterilized. -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/3000. Predicted molecular weight: 61 kDa.
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Notes |
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WB
1/1000 - 1/3000. Predicted molecular weight: 61 kDa. |
Target
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Relevance
In Brassicaceae, the enzyme myrosinase (beta-thioglucoside glucohydrolase, TGG) degrades glucosinolates to produce toxins like thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones that deter herbivory. There are two TGG enzymes, TGG1 and TGG2, which have a redundant function. -
Database links
- SwissProt: P37702 Arabidopsis thaliana
Images
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Anti-TGG1 / Myrosinase 1 antibody (ab243202) at 1/1000 dilution + Arabidopsis leaf extract at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 61 kDaThe protein undergoes modifications such as elimination of the signal peptide and glycosylation at nine positions, which changes molecular mass in the mature form.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab243202 has been referenced in 7 publications.
- Shirakawa M et al. Myrosin cell development is regulated by endocytosis machinery and PIN1 polarity in leaf primordia of Arabidopsis thaliana. Plant Cell 26:4448-61 (2014). WB ; Arabidopsis thaliana . PubMed: 25428982
- Liebminger E et al. Myrosinases TGG1 and TGG2 from Arabidopsis thaliana contain exclusively oligomannosidic N-glycans. Phytochemistry 84:24-30 (2012). WB ; Arabidopsis thaliana . PubMed: 23009876
- Hüttner S et al. Unraveling the function of Arabidopsis thaliana OS9 in the endoplasmic reticulum-associated degradation of glycoproteins. Plant Mol Biol 79:21-33 (2012). WB ; Arabidopsis thaliana . PubMed: 22328055
- Farid A et al. Arabidopsis thaliana alpha1,2-glucosyltransferase (ALG10) is required for efficient N-glycosylation and leaf growth. Plant J 68:314-25 (2011). WB ; Arabidopsis thaliana . PubMed: 21707802
- Agee AE et al. MODIFIED VACUOLE PHENOTYPE1 is an Arabidopsis myrosinase-associated protein involved in endomembrane protein trafficking. Plant Physiol 152:120-32 (2010). WB ; Arabidopsis thaliana . PubMed: 19880612
- Shirakawa M et al. Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development. Plant J 64:924-35 (2010). WB ; Arabidopsis thaliana . PubMed: 21143674
- Ueda H et al. AtVAM3 is required for normal specification of idioblasts, myrosin cells. Plant Cell Physiol 47:164-75 (2006). WB, IHC-P, Electron Microscopy . PubMed: 16306062