Overview

  • Product name

    Anti-TGG1 / Myrosinase 1 antibody
  • Description

    Rabbit polyclonal to TGG1 / Myrosinase 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Arabidopsis thaliana
  • Immunogen

    Synthetic peptide corresponding to Arabidopsis thaliana TGG1/ Myrosinase 1 aa 353-366 conjugated to bovine serum albumin.
    Sequence:

    AQNNQTIVPSDVHT


    Database link: P37702

  • Positive control

    • WB: Arabidopsis leaf extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Filter-sterilized.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab243202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. Predicted molecular weight: 61 kDa.

Target

  • Relevance

    In Brassicaceae, the enzyme myrosinase (beta-thioglucoside glucohydrolase, TGG) degrades glucosinolates to produce toxins like thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones that deter herbivory. There are two TGG enzymes, TGG1 and TGG2, which have a redundant function.
  • Database links

    Images

    • Anti-TGG1 / Myrosinase 1 antibody (ab243202) at 1/1000 dilution + Arabidopsis leaf extract

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Predicted band size: 61 kDa



      The protein undergoes modifications such as elimination of the signal peptide and glycosylation at nine positions, which changes molecular mass in the mature form.

    References

    This product has been referenced in:

    • Shirakawa M  et al. Myrosin cell development is regulated by endocytosis machinery and PIN1 polarity in leaf primordia of Arabidopsis thaliana. Plant Cell 26:4448-61 (2014). WB ; Arabidopsis thaliana . Read more (PubMed: 25428982) »
    • Liebminger E  et al. Myrosinases TGG1 and TGG2 from Arabidopsis thaliana contain exclusively oligomannosidic N-glycans. Phytochemistry 84:24-30 (2012). WB ; Arabidopsis thaliana . Read more (PubMed: 23009876) »
    See all 7 Publications for this product

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