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I would like to know the working concentration for TGIF antibody in western blotting (ab50884). Thanks.
Asked on Jan 21 2008
Thank you for your enquiry. I can confirm that this antibody was used at 1:100 for the detection of the immunogen peptide in western blotting, as shown in the image on the datasheet. I can suggest this would be a good starting dilution to try. However, this may require some optimisation in your laboratory, particularly as you will be using endogenous samples. I am sorry we do not currently have any data for use of the antibody on cell or tissue lysate. I hope this is helpful to you. Should you have any further questions, please do not hesitate to contact us.
Answered on Jan 21 2008