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WB with human samples
no signal, but not even pos. crtl (stimulated cells) gave no signal
other antibodies worked and actin was detected
Ab: 1/500, 1/50
20 ug protein loaded
block w/ Odyssee blocking buffer
secondary: Cy3, is OK
transfer: worked, other proteins detected
Asked on Jan 25 2012
Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry these 3 products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued free of charge replacements with the order number xxx for ab50884 and ab78421, and the order number xxx for ab118402 (backordered). The estimated delivery date for the latter product is xxx. I hope this is OK.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.
Answered on Jan 26 2012