Overview

  • Product name
  • Description
    Goat polyclonal to TGIF2
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human TGIF2 aa 211-220 (C terminal).
    Sequence:

    EMELQKQQDP

  • Positive control
    • A431, A549 and HEK293 cell lysates
  • General notes

    Previously labelled as TGF beta induced factor 2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab39998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa).
ELISA 1/16000.

Target

  • Function
    Transcriptional repressor, which probably repress transcription by binding directly the 5'-CTGTCAA-3' DNA sequence or by interacting with TGF-beta activated SMAD proteins. Probably represses transcription via the recruitment of histone deacetylase proteins.
  • Tissue specificity
    Widely expressed. Highly expressed in heart, kidney and testis. Weakly expressed in brain and prostate.
  • Sequence similarities
    Belongs to the TALE/TGIF homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications
    The C-terminal part is phosphorylated in response to EGF signaling by the Ras/MAPK pathway.
  • Cellular localization
    Nucleus. Excluded from nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5' TG 3' interacting factor 2 antibody
    • 5''-TG-3''-interacting factor 2 antibody
    • Homeobox protein TGIF2 antibody
    • TGF (beta) induced transcription factor 2 antibody
    • TGF beta induced factor 2 antibody
    • TGF-beta-induced transcription factor 2 antibody
    • TGFB induced factor 2 antibody
    • TGFB-induced factor 2 antibody
    • Tgif2 antibody
    • TGIF2_HUMAN antibody
    • Transforming growth factor beta induced factor 2 antibody
    see all

Images

  • Anti-TGIF2 antibody (ab39998) at 0.3 µg/ml + A431 lysate in RIPA buffer at 35 µg

    Predicted band size: 26 kDa
    Observed band size: 22 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour. Detected by chemiluminescence.

References

ab39998 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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