Overview

  • Product name

    Anti-TGIF2 antibody [EPR5655(2)]
    See all TGIF2 primary antibodies
  • Description

    Rabbit monoclonal [EPR5655(2)] to TGIF2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TGIF2 (internal sequence).
    Database link: Q9GZN2

  • Positive control

    • Human fetal kidney tissue lysate; HeLa cell lysate; Human ovary cancer tissue lysate; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Previously labelled as TGF beta induced factor 2. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Transcriptional repressor, which probably repress transcription by binding directly the 5'-CTGTCAA-3' DNA sequence or by interacting with TGF-beta activated SMAD proteins. Probably represses transcription via the recruitment of histone deacetylase proteins.
    • Tissue specificity

      Widely expressed. Highly expressed in heart, kidney and testis. Weakly expressed in brain and prostate.
    • Sequence similarities

      Belongs to the TALE/TGIF homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Post-translational
      modifications

      The C-terminal part is phosphorylated in response to EGF signaling by the Ras/MAPK pathway.
    • Cellular localization

      Nucleus. Excluded from nucleoli.
    • Information by UniProt
    • Database links

    • Alternative names

      • 5' TG 3' interacting factor 2 antibody
      • 5''-TG-3''-interacting factor 2 antibody
      • Homeobox protein TGIF2 antibody
      • TGF (beta) induced transcription factor 2 antibody
      • TGF beta induced factor 2 antibody
      • TGF-beta-induced transcription factor 2 antibody
      • TGFB induced factor 2 antibody
      • TGFB-induced factor 2 antibody
      • Tgif2 antibody
      • TGIF2_HUMAN antibody
      • Transforming growth factor beta induced factor 2 antibody
      see all

    Images

    • All lanes : Anti-TGIF2 antibody [EPR5655(2)] (ab155948) at 1/1000 dilution

      Lane 1 : Human fetal kidney tissue lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Human ovary cancer tissue lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 26 kDa

    • Immunofluorescence analysis of HeLa cells, labeling TGIF2 using ab155948 at 1/250 dilution.

    References

    This product has been referenced in:

    • Tanaka F  et al. Nuclear PKM2 promotes the progression of oral squamous cell carcinoma by inducing EMT and post-translationally repressing TGIF2. Oncotarget 9:33745-33761 (2018). Read more (PubMed: 30333907) »
    • Agrawal P  et al. A Systems Biology Approach Identifies FUT8 as a Driver of Melanoma Metastasis. Cancer Cell 31:804-819.e7 (2017). Read more (PubMed: 28609658) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Melanoma cells 4L)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    60 µg
    Specification
    Melanoma cells 4L
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted May 15 2017

    Application
    ChIP
    Sample
    Human Cell lysate - whole cell (melanoma cell lines 4L and SkMel85)
    Specification
    melanoma cell lines 4L and SkMel85
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: para formaldehyde

    Abcam user community

    Verified customer

    Submitted May 04 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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