Key features and details
- Rabbit polyclonal to TGIFLX
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TGIFLX antibody
DescriptionRabbit polyclonal to TGIFLX
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
- WB: TGIF2LX over-expression - HEK-293T whole cell lysates; IHC-P: Human testis tissue; ICC: SH-SY5Y cells.
This product was previously labelled as TGIF2LX
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 26 kDa.|
FunctionMay have a transcription role in testis.
Tissue specificitySpecifically expressed in adult testis.
Sequence similaritiesBelongs to the TALE/TGIF homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- Homeobox protein TGIF2LX antibody
- MGC130458 antibody
- testis expressed homeobox Tex1 antibody
All lanes : Anti-TGIFLX antibody (ab224490) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : TGIF2LX over-expression - HEK-293T whole cell lysate
Predicted band size: 26 kDa
Immunohistochemical analysis of human testis tissue labeling TGIFLX in the nucleus of cells in seminiferus ducts with ab224490 at 1/1000 dilution.
Immunofluorescent analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling TGIFLX (green) in the nucleoplasm with ab224490 at 2 µg/ml.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224490 has not yet been referenced specifically in any publications.