• Product name
  • Description
    Rabbit polyclonal to TGM1
  • Host species
  • Specificity
    This antibody is specific to keratinocyte Transglutaminase 1.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TGM1. A 15 residue synthetic peptide derived from a domain specific for TGM1.
    Database link: P22735


Our Abpromise guarantee covers the use of ab27000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 90 kDa.


  • Function
    Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Responsible for cross-linking epidermal proteins during formation of the stratum corneum.
  • Involvement in disease
    Defects in TGM1 are the cause of ichthyosis lamellar type 1 (LI1) [MIM:242300]. LI is a non-bullous ichthyosis, a skin disorder characterized by abnormal cornification of the epidermis. It is one the most severe forms of ichthyoses apparent at birth and persisting throughout life. LI patients are born encased in a tight, shiny, translucent covering called collodion membrane. Over the first weeks of life, the collodion membrane is gradually replaced by generalized large, dark brown, plate-like scales with minimal to no erythroderma. Tautness of facial skin commonly results in ectropion, eclabium and scarring alopecia of the scalp. Common complications are severe heat intolerance and recurrent ear infections.
    Defects in TGM1 are a cause of non-bullous congenital ichthyosiform erythroderma (NCIE) [MIM:242100]. NCIE is a non-bullous ichthyosis, a skin disorder characterized by abnormal cornification of the epidermis. Most affected individuals are born with a tight, shiny, translucent covering called collodion membrane. The collodion membrane subsequently evolves into generalized scaling and intense redness of the skin. Clinical features are milder than in lamellar ichthyoses and demonstrate a greater variability in the intensity of erythema, size and type of scales. In contrast to lamellar ichthyoses, scales are usually white, fine and powdery, and palms and soles are severely affected. Patients suffer from palmoplantar keratoderma, often with painful fissures, digital contractures, and loss of pulp volume.
    Defects in TGM1 are the cause of ichthyosis congenital autosomal recessive TGM1-related (ARCI-TGM1) [MIM:242300]. A disorder of keratinization with abnormal differentiation and desquamation of the epidermis resulting in two major clinical entities. Lamellar ichthyosis is a condition often associated with an embedment in a collodion-like membrane at birth; skin scales later develop, covering the entire body surface. Non-bullous congenital ichthyosiform erythroderma characterized by fine whitish scaling on an erythrodermal background; larger brownish scales are present on the buttocks, neck and legs.
  • Sequence similarities
    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Post-translational
    The membrane anchorage region possesses a cluster of five cysteines within which fatty acid(s) may become thioester-linked. It is subject to phorbol ester-stimulated phosphorylation and is hypersensitive to proteolysis, which releases the enzyme in a soluble form.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • ARCI1 antibody
    • Epidermal TGase antibody
    • ICR2 antibody
    • KTG antibody
    • LI antibody
    • LI1 antibody
    • Protein glutamine gamma glutamyltransferase K antibody
    • Protein-glutamine gamma-glutamyltransferase K antibody
    • TG(K) antibody
    • TGase 1 antibody
    • TGASE antibody
    • TGase K antibody
    • TGase-1 antibody
    • TGK antibody
    • TGM1 antibody
    • TGM1_HUMAN antibody
    • Transglutaminase 1 (K polypeptide epidermal type I, protein glutamine gamma glutamyltransferase) antibody
    • Transglutaminase 1 antibody
    • Transglutaminase K antibody
    • Transglutaminase, keratinocyte antibody
    • Transglutaminase-1 antibody
    see all


  • Anti-TGM1 antibody (ab27000) at 1/500 dilution + Human recombinant Transglutaminase at 0.5 µg

    HRP-conjugated anti Rabbit IgG at 1/5000 dilution

    Predicted band size: 90 kDa
    Observed band size: 90 kDa


This product has been referenced in:
  • Mallery SR  et al. Topical application of a mucoadhesive freeze-dried black raspberry gel induces clinical and histologic regression and reduces loss of heterozygosity events in premalignant oral intraepithelial lesions: results from a multicentered, placebo-controlled clinical trial. Clin Cancer Res 20:1910-24 (2014). WB ; Human . Read more (PubMed: 24486592) »
  • Grall A  et al. PNPLA1 mutations cause autosomal recessive congenital ichthyosis in golden retriever dogs and humans. Nat Genet 44:140-7 (2012). Read more (PubMed: 22246504) »

See all 2 Publications for this product

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Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (human reconstruct epidermis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate ph6
human reconstruct epidermis
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C

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Submitted Jun 28 2017


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