Product nameAnti-TGN46 antibody [2F7.1]
See all TGN46 primary antibodies
DescriptionMouse monoclonal [2F7.1] to TGN46
Tested applicationsSuitable for: IP, Inhibition Assay, WB, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Rat, Rabbit, Hamster, Human, Non human primates, African green monkey, Chinese hamster
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
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Our Abpromise guarantee covers the use of ab2809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|Inhibition Assay||Use at an assay dependent concentration.|
This antibody detects an ~38 kDa protein representing recombinant rat TGN38.
|Flow Cyt||1/20 - 1/50.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/20 - 1/50.|
FunctionMay be involved in regulating membrane traffic to and from trans-Golgi network.
Tissue specificityIsoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.
Cellular localizationCell membrane. Golgi apparatus > trans-Golgi network membrane. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes.
- Information by UniProt
- TGN 46 antibody
- TGN 51 antibody
- TGN38 antibody
ab2809 staining TGN46 (green) in HepG2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% donkey serum in 0.1% PBST for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in blocking solution) for 3 hours at 22°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal was used as the secondary antibody. F-actin (blue) was stained using CytoPainter F-actin Staining Kit - Blue Fluorescence (ab112124).
Overlay histogram showing CHO cells stained with ab2809 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2809, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was goat anti-mouse DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in CHO cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
ab2809 staining TGN46 in rat hepatocytescells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% donkey serum in 0.1% PBST for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in blocking solution) for 3 hours at 22°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal was used as the secondary antibody.
Anti-TGN46 antibody [2F7.1] (ab2809) at 1/2000 dilution + HepG2 whole cell lysate at 20 µg
HRP-conjugated donkey anti-mouse IgG polyclonal at 1/1 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 45 seconds
Blocked with 5% milk in PBST for 1 hour at 22°C.
Incubated with the primary antibody for 12 hours at 4°C in 1% BSA in PBS.
HeLa and COS7 cells were rinsed with PBS and fixed with -20°C methanol for 5 minutes. Cells were incubated with anti-TGN46 antibody (ab2809) diluted 1/100 in PBS containing 0.2% BSA for 1 hour. The cells were then rinsed in PBS and incubated with goat anti-mouse IgG conjugated to rhodamine for 30 minutes in PBS containing 0.2% BSA.
ab2809 staining Chinese Hamster Ovary cell by Immunocytochemistry/Immunofluorescence. The cells were paraformaldehyde fixed and permeabilized in 0.5% Triton prior to blocking with 10% FCS for 30 minutes at room temperature. The primary antibody was diluted 1/1000 and incubated with the sample for 2 hours. The secondary antibody was an Alexa Fluor® 488-conjugated Goat antibody, diluted 1/1000.
This product has been referenced in:
- Poulsen ET et al. An Aberrant Phosphorylation of Amyloid Precursor Protein Tyrosine Regulates Its Trafficking and the Binding to the Clathrin Endocytic Complex in Neural Stem Cells of Alzheimer's Disease Patients. Front Mol Neurosci 10:59 (2017). ICC/IF . Read more (PubMed: 28360834) »
- Han F et al. Globozoospermia and lack of acrosome formation in GM130-deficient mice. Cell Death Dis 8:e2532 (2017). Read more (PubMed: 28055014) »