Product nameAnti-TGN46 antibody
See all TGN46 primary antibodies
DescriptionRabbit polyclonal to TGN46
Tested applicationsSuitable for: ICC/IF, IHC-P, IPmore details
Species reactivityReacts with: Human
- In IF, this antibody gave a positive signal in HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab16052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||Use a concentration of 5 µg/ml.|
FunctionMay be involved in regulating membrane traffic to and from trans-Golgi network.
Tissue specificityIsoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.
Cellular localizationCell membrane. Golgi apparatus > trans-Golgi network membrane. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes.
- Information by UniProt
- TGN 46 antibody
- TGN 51 antibody
- TGN38 antibody
ICC/IF image of ab16052 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab16052, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
TGN46 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to TGN46 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16052.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 51kDa; TGN46
Image courtesy of Human Protein Atlas
ab16052 staining TGN46 in Human appendix.The paraffin embedded human skin tissue was incubated with ab16052 (1/50 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab16052 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
Immunofluorescence analysis of SH-SY5Y cells transfected with CLU2, staining TGN46 with ab16052.
Cells were fixed with ice-cold methanol and blocked with 5% goat serum. Samples were incubated with primary antibody (1/1000) overnight at 4°C. An AlexaFluor®568-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody.
This product has been referenced in:
- Ferguson R & Subramanian V The cellular uptake of angiogenin, an angiogenic and neurotrophic factor is through multiple pathways and largely dynamin independent. PLoS One 13:e0193302 (2018). Read more (PubMed: 29486010) »
- Elks CM et al. Blueberries improve glucose tolerance without altering body composition in obese postmenopausal mice. Obesity (Silver Spring) 23:573-80 (2015). Read more (PubMed: 25611327) »