Overview

  • Product name
  • Description
    Rabbit polyclonal to TGN46
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human TGN46.

    Read Abcam's proprietary immunogen policy (Peptide available as ab16378.)

  • Positive control
    • In IF, this antibody gave a positive signal in HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use a concentration of 5 µg/ml.

Target

  • Function
    May be involved in regulating membrane traffic to and from trans-Golgi network.
  • Tissue specificity
    Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.
  • Cellular localization
    Cell membrane. Golgi apparatus > trans-Golgi network membrane. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • TGN 46 antibody
    • TGN 51 antibody
    • TGN38 antibody
    • TGN38 homolog antibody
    • TGN38B antibody
    • TGN46 antibody
    • TGN48 antibody
    • TGN51 antibody
    • TGOLN2 antibody
    • TGON2_HUMAN antibody
    • Trans Golgi network integral membrane protein 2 antibody
    • Trans golgi network protein 2 antibody
    • Trans Golgi network protein TGN51 antibody
    • Trans-Golgi network integral membrane protein 2 antibody
    • Trans-Golgi network protein TGN51 antibody
    • TTGN2 antibody
    see all

Images

  • TGN46 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to TGN46 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16052.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 51kDa; TGN46

  • ICC/IF image of ab16052 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab16052, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • Image courtesy of Human Protein Atlas

    ab16052 staining TGN46 in Human appendix.The paraffin embedded human skin tissue was incubated with ab16052 (1/50 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab16052 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

    Further results for this antibody can be found at www.proteinatlas.org

  • Immunofluorescence analysis of SH-SY5Y cells transfected with CLU2, staining TGN46 with ab16052.

    Cells were fixed with ice-cold methanol and blocked with 5% goat serum. Samples were incubated with primary antibody (1/1000) overnight at 4°C. An AlexaFluor®568-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody.

References

This product has been referenced in:
  • Ferguson R & Subramanian V The cellular uptake of angiogenin, an angiogenic and neurotrophic factor is through multiple pathways and largely dynamin independent. PLoS One 13:e0193302 (2018). Read more (PubMed: 29486010) »
  • Elks CM  et al. Blueberries improve glucose tolerance without altering body composition in obese postmenopausal mice. Obesity (Silver Spring) 23:573-80 (2015). Read more (PubMed: 25611327) »
See all 15 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
African Green Monkey Cultured Cells (COS7)
Specification
COS7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.02% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jul 09 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (hippocampal culture 14 DIV)
Specification
hippocampal culture 14 DIV
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.02%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jul 06 2012

Answer

I am very pleased to hear you would like to accept our offer and test ab16052 in African Green Monkey. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forAfrican Green Monkeyand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (H1299)
Specification
H1299
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 28 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293 EBNA)
Specification
HEK293 EBNA
Fixative
3% Paraformaldehyde
Permeabilization
No
Blocking step
NH4Cl as blocking agent for 15 minute(s) · Concentration: 0.27% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 31 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela)
Specification
Hela
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% saponin
Blocking step
Casein block + 2.5% serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Sep 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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