Anti-TGN46 antibody (ab50595)
Key features and details
- Rabbit polyclonal to TGN46
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TGN46 antibody
See all TGN46 primary antibodies -
Description
Rabbit polyclonal to TGN46 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human TGN46 aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin (Cysteine residue).
Database link: O43493-2 -
Positive control
- ICC/IF: Hep G2 and A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (6) |
Use a concentration of 5 - 10 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 5 - 10 µg/ml. |
Target
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Function
May be involved in regulating membrane traffic to and from trans-Golgi network. -
Tissue specificity
Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells. -
Cellular localization
Cell membrane. Golgi apparatus > trans-Golgi network membrane. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes. - Information by UniProt
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Database links
- Entrez Gene: 10618 Human
- Entrez Gene: 192152 Rat
- Omim: 603062 Human
- SwissProt: O43493 Human
- Unigene: 593382 Human
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Alternative names
- TGN 46 antibody
- TGN 51 antibody
- TGN38 antibody
see all
Images
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ab50595 staining TGN46 in wild-type A549 cells (top panel) and TGOLN2 knockout A549 cells (bottom panel) (ab269510). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab50595 at 0.1μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
ab50595 staining TGN46 in HepG2 cells (top panel - positive control) and HeLa cells (bottom panel - negative control, low TGN46 expression). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab50595 at 1μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
Immunocytochemistry/ Immunofluorescence analysis of Panc-1 cells labeling TGN46 with ab50595 at 1/100 dilution. Cells were fixed with formaldehyde followed by blocking in 1% Donkey serum in PBST for 1 hour at 22°C. A donkey-anti-Rb Alexa Fluor® 594 was used as the secondary antibody at 1/200 dilution. DAPI nulcear counter stain.
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ab50595 staining TGN46 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde in PBS for 10 minutes, permeabilized with 0.1% Triton X100 and blocked with 2% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 2% BSA + 0.1% Triton X100) for 2 hours at 25°C. An Alexa Fluor® 594-conjugated Goat polyclonal to mouse IgG (dilution 1/1500) was used as secondary antibody.
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Human A549 cells were fixed and permeabilized with methanol followed by acetone fixation. Fixed cells were stained with 2.5 µg/ml ab50595. The antibody was developed with Goat Anti-Rabbit IgG, FITC conjugate.
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ab50595 staining human osteosarcoma (143b) cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37°C. The secondary antibody was Alexa Fluor® 594 conjugated donkey polyclonal to rabbit diluted 1/500.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (64)
ab50595 has been referenced in 64 publications.
- Maxson ME et al. Detection and quantification of the vacuolar H+ATPase using the Legionella effector protein SidK. J Cell Biol 221:N/A (2022). PubMed: 35024770
- Guo Z et al. Activity-dependent PI4P synthesis by PI4KIIIα regulates long-term synaptic potentiation. Cell Rep 38:110452 (2022). PubMed: 35235793
- Lott J et al. The Synthetic Cannabinoid WIN55,212-2 Can Disrupt the Golgi Apparatus Independent of Cannabinoid Receptor-1. Mol Pharmacol 101:371-380 (2022). PubMed: 35236771
- Husein D et al. Identification of the C-terminal region in Amelogenesis Imperfecta causative protein WDR72 required for Golgi localization. Sci Rep 12:4640 (2022). PubMed: 35301423
- Jaldin-Fincati J et al. Aluminum hydroxide adjuvant diverts the uptake and trafficking of genetically detoxified pertussis toxin to lysosomes in macrophages. Mol Microbiol 117:1173-1195 (2022). PubMed: 35344242