Recombinant Anti-TGN46 antibody [EPR12676] (ab174280)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12676] to TGN46
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-TGN46 antibody [EPR12676]
See all TGN46 primary antibodies -
Description
Rabbit monoclonal [EPR12676] to TGN46 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human TGN46 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: O43493 -
Positive control
- Human fetal liver, fetal lung, fetal kidney lysates. HeLa lysates. Human brain and kidney tissues. Immunoprecipitation pellet from fetal liver lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12676 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab174280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 51 kDa. | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May be involved in regulating membrane traffic to and from trans-Golgi network. -
Tissue specificity
Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells. -
Cellular localization
Cell membrane. Golgi apparatus > trans-Golgi network membrane. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes. - Information by UniProt
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Database links
- Entrez Gene: 10618 Human
- Omim: 603062 Human
- SwissProt: O43493 Human
- Unigene: 593382 Human
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Alternative names
- TGN 46 antibody
- TGN 51 antibody
- TGN38 antibody
see all
Images
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![Western blot - Anti-TGN46 antibody [EPR12676] (ab174280)](https://www.abcam.com/ps/products/174/ab174280/Images/ab174280-199270-anti-tgn46-antibody-epr12676-western-blot.jpg)
Western blot - Anti-TGN46 antibody [EPR12676] (ab174280)All lanes : Anti-TGN46 antibody [EPR12676] (ab174280) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal lung lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 51 kDa -
![Immunoprecipitation - Anti-TGN46 antibody [EPR12676] (ab174280)](https://www.abcam.com/ps/products/174/ab174280/Images/ab174280-199266-anti-tgn46-antibody-epr12676-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TGN46 antibody [EPR12676] (ab174280)Immunoprecipitation. ab174280 at 1/1000 labeling TGN46 immunoprecipitated from Human fetal liver lysate using ab174280 at 1/10.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)](https://www.abcam.com/ps/products/174/ab174280/Images/ab174280-199264-anti-tgn46-antibody-epr12676-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)Immunohistochemical analysis of paraffin embedded Human brain tissue labeling TGN46 with ab174280 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)](https://www.abcam.com/ps/products/174/ab174280/Images/ab174280-199263-anti-tgn46-antibody-epr12676-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling TGN46 with ab174280 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References
ab174280 has been referenced in 1 publication.
- Han J et al. HSP90 inhibition targets autophagy and induces a CASP9-dependent resistance mechanism in NSCLC. Autophagy 14:958-971 (2018). PubMed: 29561705
