Recombinant
RabMAb

Anti-TGN46 antibody [EPR12676] (ab174280)

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Overview

  • Product name
    Anti-TGN46 antibody [EPR12676]
    See all TGN46 primary antibodies
  • Description
    Rabbit monoclonal [EPR12676] to TGN46
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TGN46 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: O43493

  • Positive control
    • Human fetal liver, fetal lung, fetal kidney lysates. HeLa lysates. Human brain and kidney tissues. Immunoprecipitation pellet from fetal liver lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12676
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 51 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.

    • Target

    • Function
      May be involved in regulating membrane traffic to and from trans-Golgi network.
    • Tissue specificity
      Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.
    • Cellular localization
      Cell membrane. Golgi apparatus > trans-Golgi network membrane. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes.
    • Information by UniProt
    • Database links
      • Alternative names
        • TGN 46 antibody
        • TGN 51 antibody
        • TGN38 antibody
        • TGN38 homolog antibody
        • TGN46 antibody
        • TGN48 antibody
        • TGN51 antibody
        • TGOLN2 antibody
        • TGON2_HUMAN antibody
        • Trans Golgi network integral membrane protein 2 antibody
        • Trans golgi network protein 2 antibody
        • Trans Golgi network protein TGN51 antibody
        • Trans-Golgi network integral membrane protein 2 antibody
        • Trans-Golgi network protein TGN51 antibody
        • TTGN2 antibody
        see all

      Images

      • Western blot - Anti-TGN46 antibody [EPR12676] (ab174280)
        Western blot - Anti-TGN46 antibody [EPR12676] (ab174280)
        All lanes : Anti-TGN46 antibody [EPR12676] (ab174280) at 1/1000 dilution

        Lane 1 : Human fetal liver lysate
        Lane 2 : Human fetal lung lysate
        Lane 3 : Human fetal kidney lysate
        Lane 4 : HeLa lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 51 kDa

      • Immunoprecipitation - Anti-TGN46 antibody [EPR12676] (ab174280)
        Immunoprecipitation - Anti-TGN46 antibody [EPR12676] (ab174280)

        Immunoprecipitation. ab174280 at 1/1000 labeling TGN46 immunoprecipitated from Human fetal liver lysate using ab174280 at 1/10.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)

        Immunohistochemical analysis of paraffin embedded Human brain tissue labeling TGN46 with ab174280 at 1/50.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)

        Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling TGN46 with ab174280 at 1/50.

      References

      ab174280 has not yet been referenced specifically in any publications.

      Publishing research using ab174280? Please let us know so that we can cite the reference in this datasheet.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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