Recombinant
RabMAb

Recombinant Anti-TGN46 antibody [EPR12676] (ab174280)

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Overview

  • Product name

    Anti-TGN46 antibody [EPR12676]
    See all TGN46 primary antibodies

  • Description

    Rabbit monoclonal [EPR12676] to TGN46

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human TGN46 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: O43493

  • Positive control

    • Human fetal liver, fetal lung, fetal kidney lysates. HeLa lysates. Human brain and kidney tissues. Immunoprecipitation pellet from fetal liver lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12676

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 51 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.

    • Target

    • Function

      May be involved in regulating membrane traffic to and from trans-Golgi network.

    • Tissue specificity

      Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.

    • Cellular localization

      Cell membrane. Golgi apparatus > trans-Golgi network membrane. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes.
    • Information by UniProt

    • Database links

      • Alternative names

        • TGN 46 antibody
        • TGN 51 antibody
        • TGN38 antibody
        • TGN38 homolog antibody
        • TGN38B antibody
        • TGN46 antibody
        • TGN48 antibody
        • TGN51 antibody
        • TGOLN2 antibody
        • TGON2_HUMAN antibody
        • Trans Golgi network integral membrane protein 2 antibody
        • Trans golgi network protein 2 antibody
        • Trans Golgi network protein TGN51 antibody
        • Trans-Golgi network integral membrane protein 2 antibody
        • Trans-Golgi network protein TGN51 antibody
        • TTGN2 antibody
        see all

      Images

      • Western blot - Anti-TGN46 antibody [EPR12676] (ab174280)
        Western blot - Anti-TGN46 antibody [EPR12676] (ab174280)
        All lanes : Anti-TGN46 antibody [EPR12676] (ab174280) at 1/1000 dilution

        Lane 1 : Human fetal liver lysate
        Lane 2 : Human fetal lung lysate
        Lane 3 : Human fetal kidney lysate
        Lane 4 : HeLa lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 51 kDa

      • Immunoprecipitation - Anti-TGN46 antibody [EPR12676] (ab174280)
        Immunoprecipitation - Anti-TGN46 antibody [EPR12676] (ab174280)

        Immunoprecipitation. ab174280 at 1/1000 labeling TGN46 immunoprecipitated from Human fetal liver lysate using ab174280 at 1/10.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)

        Immunohistochemical analysis of paraffin embedded Human brain tissue labeling TGN46 with ab174280 at 1/50.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)

        Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling TGN46 with ab174280 at 1/50.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      References

      ab174280 has not yet been referenced specifically in any publications.

      Publishing research using ab174280? Please let us know so that we can cite the reference in this datasheet.

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