Anti-TGN46 antibody [EPR12676] (ab174280)
- Datasheet
- References
- Protocols
Overview
-
Product nameAnti-TGN46 antibody [EPR12676]
See all TGN46 primary antibodies -
DescriptionRabbit monoclonal [EPR12676] to TGN46
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivityReacts with: Human
-
Immunogen
Synthetic peptide within Human TGN46 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: O43493 -
Positive control
- Human fetal liver, fetal lung, fetal kidney lysates. HeLa lysates. Human brain and kidney tissues. Immunoprecipitation pellet from fetal liver lysate.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR12676
-
IsotypeIgG
-
Research areas
Associated products
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab174280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 51 kDa. | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
FunctionMay be involved in regulating membrane traffic to and from trans-Golgi network.
-
Tissue specificityIsoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.
-
Cellular localizationCell membrane. Golgi apparatus > trans-Golgi network membrane. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes.
- Information by UniProt
-
Database links
- Entrez Gene: 10618 Human
- Omim: 603062 Human
- SwissProt: O43493 Human
- Unigene: 593382 Human
-
Alternative names
- TGN 46 antibody
- TGN 51 antibody
- TGN38 antibody
see all
Images
-
![Western blot - Anti-TGN46 antibody [EPR12676] (ab174280)](https://www.abcam.com/ps/products/174/ab174280/Images/ab174280-199270-ab174280.jpg)
Western blot - Anti-TGN46 antibody [EPR12676] (ab174280)All lanes : Anti-TGN46 antibody [EPR12676] (ab174280) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal lung lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 51 kDa -
![Immunoprecipitation - Anti-TGN46 antibody [EPR12676] (ab174280)](https://www.abcam.com/ps/products/174/ab174280/Images/ab174280-199266-ab174280d.jpg)
Immunoprecipitation - Anti-TGN46 antibody [EPR12676] (ab174280)Immunoprecipitation. ab174280 at 1/1000 labeling TGN46 immunoprecipitated from Human fetal liver lysate using ab174280 at 1/10.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)](https://www.abcam.com/ps/products/174/ab174280/Images/ab174280-199264-ab174280b.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)Immunohistochemical analysis of paraffin embedded Human brain tissue labeling TGN46 with ab174280 at 1/50.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)](https://www.abcam.com/ps/products/174/ab174280/Images/ab174280-199263-ab174280c.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling TGN46 with ab174280 at 1/50.
Datasheets and documents
References
ab174280 has not yet been referenced specifically in any publications.
