Recombinant Anti-TH1L antibody [EPR11594] (ab170934)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11594] to TH1L
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TH1L antibody [EPR11594]
See all TH1L primary antibodies -
Description
Rabbit monoclonal [EPR11594] to TH1L -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human TH1L. The exact sequence is proprietary.
Database link: Q8IXH7 -
Positive control
- Jurkat, HeLa, MCF-7 and HCT-116 cell lysates, HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11594 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 66 kDa.
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ICC/IF |
1/50 - 1/100.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 66 kDa. |
ICC/IF
1/50 - 1/100. |
Target
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Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. -
Tissue specificity
Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal and cardiac muscle, adrenal, thyroid, kidney and pancreas. -
Sequence similarities
Belongs to the NELF-D family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 51497 Human
- Entrez Gene: 57314 Mouse
- Entrez Gene: 679203 Rat
- Omim: 605297 Human
- SwissProt: Q8IXH7 Human
- SwissProt: Q922L6 Mouse
- Unigene: 517148 Human
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Alternative names
- HSPC130 antibody
- Negative elongation factor C/D antibody
- NELF C antibody
see all
Images
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All lanes : Anti-TH1L antibody [EPR11594] (ab170934) at 1/1000 dilution
Lane 1 : Jurkat cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : MCF-7 cell lysates
Lane 4 : HCT-116 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 66 kDa -
Immunofluorescence analysis of HeLa cells labeling TH1L with ab170934 at 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab170934 has been referenced in 1 publication.
- Abe K et al. Distinct patterns of RNA polymerase II and transcriptional elongation characterize mammalian genome activation. Cell Rep 41:111865 (2022). PubMed: 36577375