Anti-TH1L antibody [EPR11594] (ab170934)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-TH1L antibody [EPR11594]
See all TH1L primary antibodies -
DescriptionRabbit monoclonal [EPR11594] to TH1L
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment within Human TH1L. The exact sequence is proprietary.
Database link: Q8IXH7 -
Positive control
- Jurkat, HeLa, MCF-7 and HCT-116 cell lysates, HeLa cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR11594
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab170934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 66 kDa. | |
| ICC/IF | 1/50 - 1/100. |
Target
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FunctionEssential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
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Tissue specificityWidely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal and cardiac muscle, adrenal, thyroid, kidney and pancreas.
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Sequence similaritiesBelongs to the NELF-D family.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 51497 Human
- Entrez Gene: 57314 Mouse
- Entrez Gene: 679203 Rat
- Omim: 605297 Human
- SwissProt: Q8IXH7 Human
- SwissProt: Q922L6 Mouse
- Unigene: 517148 Human
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Alternative names
- HSPC130 antibody
- Negative elongation factor C/D antibody
- NELF C antibody
see all
Images
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![Western blot - Anti-TH1L antibody [EPR11594] (ab170934)](https://www.abcam.com/ps/products/170/ab170934/Images/ab170934-171355-79041wb21996medium.jpg)
Western blot - Anti-TH1L antibody [EPR11594] (ab170934)All lanes : Anti-TH1L antibody [EPR11594] (ab170934) at 1/1000 dilution
Lane 1 : Jurkat cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : MCF-7 cell lysates
Lane 4 : HCT-116 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 66 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-TH1L antibody [EPR11594] (ab170934)](https://www.abcam.com/ps/products/170/ab170934/Images/ab170934-171354-79041icc21997medium.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TH1L antibody [EPR11594] (ab170934)Immunofluorescence analysis of HeLa cells labeling TH1L with ab170934 at 1/50 dilution.
Datasheets and documents
References
ab170934 has not yet been referenced specifically in any publications.
