Recombinant Anti-TH1L antibody [EPR11594] (ab170934)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11594] to TH1L
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TH1L antibody [EPR11594]
See all TH1L primary antibodies -
Description
Rabbit monoclonal [EPR11594] to TH1L -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human TH1L. The exact sequence is proprietary.
Database link: Q8IXH7 -
Positive control
- Jurkat, HeLa, MCF-7 and HCT-116 cell lysates, HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11594 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab170934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/5000. Predicted molecular weight: 66 kDa. | |
ICC/IF | 1/50 - 1/100. |
Target
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Function
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. -
Tissue specificity
Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal and cardiac muscle, adrenal, thyroid, kidney and pancreas. -
Sequence similarities
Belongs to the NELF-D family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 51497 Human
- Entrez Gene: 57314 Mouse
- Entrez Gene: 679203 Rat
- Omim: 605297 Human
- SwissProt: Q8IXH7 Human
- SwissProt: Q922L6 Mouse
- Unigene: 517148 Human
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Alternative names
- HSPC130 antibody
- Negative elongation factor C/D antibody
- NELF C antibody
see all
Images
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All lanes : Anti-TH1L antibody [EPR11594] (ab170934) at 1/1000 dilution
Lane 1 : Jurkat cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : MCF-7 cell lysates
Lane 4 : HCT-116 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 66 kDa -
Immunofluorescence analysis of HeLa cells labeling TH1L with ab170934 at 1/50 dilution.
Protocols
Datasheets and documents
References (0)
ab170934 has not yet been referenced specifically in any publications.