The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Is unsuitable for WB.
Transcriptional repressor that plays a central role for embryogenesis and the pluripotency of embryonic stem (ES) cells. Sequence-specific DNA-binding factor that represses gene expression in pluripotent ES cells by directly binding to key genetic loci and recruiting epigenetic modifiers.
Belongs to the THAP11 family. Contains 1 THAP-type zinc finger.
Nucleus. Cytoplasm. May be regulated by shuttling of the protein between the cytoplasm and nucleus.
Detection of THAP11 by Western Blot of Immunprecipitate.
An antibody to THAP11 at 1µg/ml staining THAP11 in HeLa whole cell lysate immunoprecipitated using ab113210 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.