Anti-THAP11 antibody (ab154942)
Key features and details
- Rabbit polyclonal to THAP11
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-THAP11 antibody
See all THAP11 primary antibodies -
Description
Rabbit polyclonal to THAP11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-271 of Human THAP11 (Uniprot ID: Q96EK4)
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Positive control
- NT2D1, IMR32, U87-MG, MCF 7 whole cell lysate; Hela, RT2 or C2C12 xenograft tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 34 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 34 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0). |
Target
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Function
Transcriptional repressor that plays a central role for embryogenesis and the pluripotency of embryonic stem (ES) cells. Sequence-specific DNA-binding factor that represses gene expression in pluripotent ES cells by directly binding to key genetic loci and recruiting epigenetic modifiers. -
Sequence similarities
Belongs to the THAP11 family.
Contains 1 THAP-type zinc finger. -
Cellular localization
Nucleus. Cytoplasm. May be regulated by shuttling of the protein between the cytoplasm and nucleus. - Information by UniProt
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Database links
- Entrez Gene: 539845 Cow
- Entrez Gene: 57215 Human
- Entrez Gene: 59016 Mouse
- Omim: 609119 Human
- SwissProt: A5PKF5 Cow
- SwissProt: Q96EK4 Human
- SwissProt: Q9JJD0 Mouse
- Unigene: 632200 Human
see all -
Alternative names
- CTG B43a antibody
- CTG B45d antibody
- HRIHFB2206 antibody
see all
Images
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Anti-THAP11 antibody (ab154942) at 1/3000 dilution + NT2D1 whole cell lysate at 30 µg
Predicted band size: 34 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human RT2 xenograft, labeling THAP11 using ab154942 at 1/500.
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Immunohistochemical analysis of paraffin-embedded Hela xenograft, labeling THAP11 using ab154942 at 1/500.
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Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, labeling THAP11 using ab154942 at 1/500.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154942 has not yet been referenced specifically in any publications.