Overview

  • Product name
  • Description
    Rabbit polyclonal to THAP11
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-271 of Human THAP11 (Uniprot ID: Q96EK4)

  • Positive control
    • NT2D1, IMR32, U87-MG, MCF 7 whole cell lysate; Hela, RT2 or C2C12 xenograft tissue
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 34 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Target

  • Function
    Transcriptional repressor that plays a central role for embryogenesis and the pluripotency of embryonic stem (ES) cells. Sequence-specific DNA-binding factor that represses gene expression in pluripotent ES cells by directly binding to key genetic loci and recruiting epigenetic modifiers.
  • Sequence similarities
    Belongs to the THAP11 family.
    Contains 1 THAP-type zinc finger.
  • Cellular localization
    Nucleus. Cytoplasm. May be regulated by shuttling of the protein between the cytoplasm and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CTG B43a antibody
    • CTG B45d antibody
    • HRIHFB2206 antibody
    • RONIN antibody
    • THA11_HUMAN antibody
    • THAP 11 antibody
    • THAP domain containing 11 antibody
    • THAP domain-containing protein 11 antibody
    • Thap11 antibody
    see all

Images

  • Anti-THAP11 antibody (ab154942) at 1/3000 dilution + NT2D1 whole cell lysate at 30 µg

    Predicted band size: 34 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human RT2 xenograft, labeling THAP11 using ab154942 at 1/500.
  • Immunohistochemical analysis of paraffin-embedded Hela xenograft, labeling THAP11 using ab154942 at 1/500.
  • Immunohistochemical analysis of paraffin-embedded Human C2C12 xenograft, labeling THAP11 using ab154942 at 1/500.

References

ab154942 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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