Overview

  • Product name

  • Description

    Rabbit polyclonal to Themis
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Themis aa 547-641.
    Database link: Q8N1K5

  • Positive control

    • WB: Jurkat and K562 whole cell lysate. IHC-P: Human spleen tissue. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab237626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000.
ICC/IF 1/50 - 1/200.
IHC-P 1/200 - 1/500.

Target

  • Function

    Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
  • Sequence similarities

    Belongs to the themis family.
  • Post-translational
    modifications

    Phosphorylated on Tyr residues quickly after TCR stimulation.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C6orf190 antibody
    • C6orf207 antibody
    • MGC163388 antibody
    • Protein THEMIS antibody
    • SIGNALING PHOSPHOPROTEIN SPECIFIC FOR T CELLS antibody
    • SPOT antibody
    • THEMIS antibody
    • THMS1_HUMAN antibody
    • Thymocyte expressed molecule involved in selection antibody
    • Thymocyte selection associated antibody
    • Thymocyte selection pathway associated antibody
    • Thymocyte-expressed molecule involved in selection antibody
    • TSEPA antibody
    see all

Images

  • All lanes : Anti-Themis antibody (ab237626) at 1/500 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
  • Paraffin-embedded human spleen tissue stained for Themis with ab237626 at a 1/300 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Themis using ab237626 at a dilution of 1/100 in ICC/IF.

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

References

ab237626 has not yet been referenced specifically in any publications.

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