Key features and details
- Rabbit polyclonal to Themis
- Suitable for: WB, IP
- Isotype: IgG
Product nameAnti-Themis antibody
See all Themis primary antibodies
DescriptionRabbit polyclonal to Themis
Tested applicationsSuitable for: WB, IPmore details
Synthetic peptide within Human Themis aa 591-641. The exact sequence is proprietary.
Database link: Q8N1K5
- WB: Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241476 was affinity purified using an epitope specific to Themis immobilized on solid support.
Our Abpromise guarantee covers the use of ab241476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 73 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPlays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
Sequence similaritiesBelongs to the themis family.
modificationsPhosphorylated on Tyr residues quickly after TCR stimulation.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- C6orf190 antibody
- C6orf207 antibody
- MGC163388 antibody
All lanes : Anti-Themis antibody (ab241476) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 73 kDa
Exposure time: 10 seconds
Lysates prepared using NETN lysis buffer.
Themis was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241476 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241476 at 1 µg/ml.
Lane 1: ab241476 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Lysates prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241476 has not yet been referenced specifically in any publications.