Anti-Themis antibody (ab241476)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Themis antibody
See all Themis primary antibodies -
Description
Rabbit polyclonal to Themis -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Immunogen
Synthetic peptide within Human Themis aa 591-641. The exact sequence is proprietary.
Database link: Q8N1K5 -
Positive control
- WB: Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241476 was affinity purified using an epitope specific to Themis immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab241476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 73 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk. -
Sequence similarities
Belongs to the themis family. -
Post-translational
modificationsPhosphorylated on Tyr residues quickly after TCR stimulation. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Alternative names
- C6orf190 antibody
- C6orf207 antibody
- MGC163388 antibody
see all
Images
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Western blot - Anti-Themis antibody (ab241476)All lanes : Anti-Themis antibody (ab241476) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 73 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Themis antibody (ab241476)Themis was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241476 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241476 at 1 µg/ml.
Lane 1: ab241476 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
Lysates prepared using NETN lysis buffer.
Datasheets and documents
References
ab241476 has not yet been referenced specifically in any publications.
