Anti-Themis antibody (ab241476)
Key features and details
- Rabbit polyclonal to Themis
- Suitable for: WB, IP
- Isotype: IgG
Overview
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Product name
Anti-Themis antibody
See all Themis primary antibodies -
Description
Rabbit polyclonal to Themis -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Immunogen
Synthetic peptide within Human Themis aa 591-641. The exact sequence is proprietary.
Database link: Q8N1K5 -
Positive control
- WB: Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241476 was affinity purified using an epitope specific to Themis immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab241476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 73 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk. -
Sequence similarities
Belongs to the themis family. -
Post-translational
modificationsPhosphorylated on Tyr residues quickly after TCR stimulation. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Alternative names
- C6orf190 antibody
- C6orf207 antibody
- MGC163388 antibody
see all
Images
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Western blot - Anti-Themis antibody (ab241476)All lanes : Anti-Themis antibody (ab241476) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 73 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Themis antibody (ab241476)Themis was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241476 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241476 at 1 µg/ml.
Lane 1: ab241476 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
Lysates prepared using NETN lysis buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab241476 has not yet been referenced specifically in any publications.
