Product nameAnti-Themis antibody [EPR7353]
See all Themis primary antibodies
DescriptionRabbit monoclonal [EPR7353] to Themis
Tested applicationsSuitable for: WB, IP, ICC, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide, corresponding to residues in Human Themis (Q8N1K5).
- Jurkat and Human thymus lysates; Jurkat cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab126771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 73 kDa.|
|IP||1/10 - 1/100.|
|ICC||1/50 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPlays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
Sequence similaritiesBelongs to the themis family.
modificationsPhosphorylated on Tyr residues quickly after TCR stimulation.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- C6orf190 antibody
- C6orf207 antibody
- MGC163388 antibody
All lanes : Anti-Themis antibody [EPR7353] (ab126771) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Human thymus lysates
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 73 kDa
ab126771 at 1/10 dilution staining Themis in permeabilized Jurkat cells by Flow cytometry (red) compared with a rabbit IgG negative control (green).