Overview

  • Product name

    Anti-Themis antibody [EPR7354]
    See all Themis primary antibodies
  • Description

    Rabbit monoclonal [EPR7354] to Themis
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Themis aa 600-700. The exact sequence is proprietary.

  • Positive control

    • Human thymus and Jurkat lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
    • Sequence similarities

      Belongs to the themis family.
    • Post-translational
      modifications

      Phosphorylated on Tyr residues quickly after TCR stimulation.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • C6orf190 antibody
      • C6orf207 antibody
      • MGC163388 antibody
      • Protein THEMIS antibody
      • SIGNALING PHOSPHOPROTEIN SPECIFIC FOR T CELLS antibody
      • SPOT antibody
      • THEMIS antibody
      • THMS1_HUMAN antibody
      • Thymocyte expressed molecule involved in selection antibody
      • Thymocyte selection associated antibody
      • Thymocyte selection pathway associated antibody
      • Thymocyte-expressed molecule involved in selection antibody
      • TSEPA antibody
      see all

    Images

    • All lanes : Anti-Themis antibody [EPR7354] (ab129174) at 1/10000 dilution

      Lane 1 : Human thymus lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 73 kDa
      Observed band size: 73 kDa

    References

    This product has been referenced in:

    • Davies JL  et al. Increased THEMIS First Exon Usage in CD4+ T-Cells Is Associated with a Genotype that Is Protective against Multiple Sclerosis. PLoS One 11:e0158327 (2016). WB ; Human . Read more (PubMed: 27438997) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab129174.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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