Key features and details
- Membrane-permeable dye for assessing cell viability and proliferation
- CAS Number: 298-93-1
- Purity: > 98%
- Soluble in water to 5 mg/ml
- Form / State: Solid
- Source: Synthetic
Product nameThiazolyl blue tetrazolium bromide (MTT), membrane-permeable dye
DescriptionMembrane-permeable dye for assessing cell viability and proliferation
- 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
- Methylthiazolyldiphenyl-tetrazolium bromide
Membrane-permeable dye used in measurement of cell proliferation. It produces a yellowish tetrazole solution that is converted to dark blue, water-insoluble MTT formazan by intracellular NAD(P)H-oxidoreductases of living cells. The blue crystals are solubilized and the intensity is measured colorimetrically at 570 nm.
Chemical name2-(4,5-Dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide
Storage instructionsStore at +4°C. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 5 mg/ml
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
2D chemical structure image of ab146345, Thiazolyl blue tetrazolium bromide (MTT), membrane-permeable dye
NaHS protects viability of HEI-OC1 cells upon gentamicin toxicity.
Gentamicin (GM) was added to the culture medium to a concentration of 0 (control), 1, 2.5 and 5 mM respectively incubated for 48 hours. Representative culture images (A) and viability measured by MTT assay (B) of HEI-OC1 cell culture after gentamicin (GM) treatment were shown.
Scale bar 100 μm. Values were represented as the mean ± SEM from three independent experiments. * P < 0.05 vs control.
(After Figure 1A & B of Dong et al).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab146345 has been referenced in 12 publications.
- Ablinger M et al. Personalized Development of Antisense Oligonucleotides for Exon Skipping Restores Type XVII Collagen Expression in Junctional Epidermolysis Bullosa. Int J Mol Sci 22:N/A (2021). PubMed: 33805154
- Cheng CY et al. Epigallocatechin-3-Gallate-Loaded Liposomes Favor Anti-Inflammation of Microglia Cells and Promote Neuroprotection. Int J Mol Sci 22:N/A (2021). PubMed: 33809762
- Jones O et al. The synergistic effect of Canady Helios cold atmospheric plasma and a FOLFIRINOX regimen for the treatment of cholangiocarcinoma in vitro. Sci Rep 11:8967 (2021). PubMed: 33903679
- Hasemann G et al. Refractory Metal Coated Alumina Foams as Support Material for Stem Cell and Fibroblasts Cultivation. Materials (Basel) 14:N/A (2021). PubMed: 34070481
- Chang LC et al. The Potential Effect of Fucoidan on Inhibiting Epithelial-to-Mesenchymal Transition, Proliferation, and Increase in Apoptosis for Endometriosis Treatment: In Vivo and In Vitro Study. Biomedicines 8:N/A (2020). PubMed: 33266505