Thiazolyl blue tetrazolium bromide (MTT), membrane-permeable dye (ab146345)

Overview

  • Product name
    Thiazolyl blue tetrazolium bromide (MTT), membrane-permeable dye
  • Description
    Membrane-permeable dye for assessing cell viability and proliferation
  • Alternative names
    • 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
    • Methylthiazolyldiphenyl-tetrazolium bromide
    • MTT
  • Biological description

    Membrane-permeable dye used in measurement of cell proliferation. It produces a yellowish tetrazole solution that is converted to dark blue, water-insoluble MTT formazan by intracellular NAD(P)H-oxidoreductases of living cells. The blue crystals are solubilized and the intensity is measured colorimetrically at 570 nm.

  • CAS Number
    298-93-1
  • Chemical structure
    Chemical Structure

Properties

  • Emission
    570nm
  • Chemical name
    2-(4,5-Dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide
  • Molecular weight
    414.33
  • Molecular formula
    C18H16BrN5S
  • Storage instructions
    Store at +4°C. The product can be stored for up to 12 months.
  • Solubility overview
    Soluble in water to 5 mg/ml
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

Images

  • NaHS protects viability of HEI-OC1 cells upon gentamicin toxicity.

    Gentamicin (GM) was added to the culture medium to a concentration of 0 (control), 1, 2.5 and 5 mM respectively incubated for 48 hours. Representative culture images (A) and viability measured by MTT assay (B) of HEI-OC1 cell culture after gentamicin (GM) treatment were shown.

    Scale bar 100 μm. Values were represented as the mean ± SEM from three independent experiments. * P < 0.05 vs control.

    (After Figure 1A & B of Dong et al).

References

This product has been referenced in:
  • Dong Y  et al. NaHS Protects Cochlear Hair Cells from Gentamicin-Induced Ototoxicity by Inhibiting the Mitochondrial Apoptosis Pathway. PLoS One 10:e0136051 (2015). Read more (PubMed: 26295804) »
  • Shin K  et al. TH2 cells and their cytokines regulate formation and function of lymphatic vessels. Nat Commun 6:6196 (2015). Read more (PubMed: 25648335) »
  • Wang XW & Xu YH Expression differences in TEL-AML1 fusion gene in leukemia glucocorticoid-sensitive and -resistant cell lines. Genet Mol Res 14:7883-93 (2015). Read more (PubMed: 26214469) »
  • Zahedifard M  et al. Synthesis, characterization and apoptotic activity of quinazolinone Schiff base derivatives toward MCF-7 cells via intrinsic and extrinsic apoptosis pathways. Sci Rep 5:11544 (2015). Read more (PubMed: 26108872) »
  • Berridge MV  et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev 11:127-52 (2005). Read more (PubMed: 16216776) »
See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab146345.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

Sign up