Thiazolyl blue tetrazolium bromide (MTT), membrane-permeable dye (ab146345)
Key features and details
- Membrane-permeable dye for assessing cell viability and proliferation
- CAS Number: 298-93-1
- Purity: > 98%
- Soluble in water to 5 mg/ml
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Thiazolyl blue tetrazolium bromide (MTT), membrane-permeable dye -
Description
Membrane-permeable dye for assessing cell viability and proliferation -
Alternative names
- 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
- Methylthiazolyldiphenyl-tetrazolium bromide
- MTT
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Purity
> 98% -
CAS Number
298-93-1 -
Chemical structure
Properties
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Emission
570nm -
Chemical name
2-(4,5-Dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide -
Molecular weight
414.33 -
Molecular formula
C18H16BrN5S -
Storage instructions
Store at +4°C. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 5 mg/ml -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic
Images
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2D chemical structure image of ab146345, Thiazolyl blue tetrazolium bromide (MTT), membrane-permeable dye
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NaHS protects viability of HEI-OC1 cells upon gentamicin toxicity.
Gentamicin (GM) was added to the culture medium to a concentration of 0 (control), 1, 2.5 and 5 mM respectively incubated for 48 hours. Representative culture images (A) and viability measured by MTT assay (B) of HEI-OC1 cell culture after gentamicin (GM) treatment were shown.
Scale bar 100 μm. Values were represented as the mean ± SEM from three independent experiments. * P < 0.05 vs control.
(After Figure 1A & B of Dong et al).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab146345 has been referenced in 16 publications.
- Lesslich HM et al. Adjusting the neuron to astrocyte ratio with cytostatics in hippocampal cell cultures from postnatal rats: A comparison of cytarabino furanoside (AraC) and 5-fluoro-2'-deoxyuridine (FUdR). PLoS One 17:e0265084 (2022). PubMed: 35263366
- Schröder SK et al. Lipocalin-2 (LCN2) Deficiency Leads to Cellular Changes in Highly Metastatic Human Prostate Cancer Cell Line PC-3. Cells 11:N/A (2022). PubMed: 35053376
- Poh HX et al. Alternative splicing of METTL3 explains apparently METTL3-independent m6A modifications in mRNA. PLoS Biol 20:e3001683 (2022). PubMed: 35853000
- Ablinger M et al. Personalized Development of Antisense Oligonucleotides for Exon Skipping Restores Type XVII Collagen Expression in Junctional Epidermolysis Bullosa. Int J Mol Sci 22:N/A (2021). PubMed: 33805154
- Cheng CY et al. Epigallocatechin-3-Gallate-Loaded Liposomes Favor Anti-Inflammation of Microglia Cells and Promote Neuroprotection. Int J Mol Sci 22:N/A (2021). PubMed: 33809762