Recombinant Anti-Thimet Oligopeptidase antibody [EPR7651] (ab154173)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7651] to Thimet Oligopeptidase
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Thimet Oligopeptidase antibody [EPR7651]
See all Thimet Oligopeptidase primary antibodies -
Description
Rabbit monoclonal [EPR7651] to Thimet Oligopeptidase -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Thimet Oligopeptidase aa 650 to the C-terminus. The exact sequence is proprietary.
Database link: P52888 -
Positive control
- Human cerebellum, fetal brain, K562, SH-SY5Y, THP1 and MCF7 lysates; MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7651 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 79 kDa.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 79 kDa. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation. Able to degrade the beta-amyloid precursor protein and generate amyloidogenic fragments. -
Sequence similarities
Belongs to the peptidase M3 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7064 Human
- Entrez Gene: 50492 Mouse
- Entrez Gene: 64517 Rat
- Omim: 601117 Human
- SwissProt: P52888 Human
- SwissProt: Q8C1A5 Mouse
- SwissProt: P24155 Rat
- Unigene: 78769 Human
see all -
Alternative names
- EC 3.4.24.15 antibody
- Endopeptidase 24.15 antibody
- EP24.15 antibody
see all
Images
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All lanes : Anti-Thimet Oligopeptidase antibody [EPR7651] (ab154173) at 1/1000 dilution
Lane 1 : Human cerebellum lysate
Lane 2 : Human fetal brain lysate
Lane 3 : K562 cell lysate
Lane 4 : SH-SY5Y cell lysate
Lane 5 : THP1 cell lysate
Lane 6 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 79 kDa -
Immunofluorescence analysis of MCF7 cells labeling Thimet Oligopeptidase with ab154173 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154173 has not yet been referenced specifically in any publications.