Recombinant
RabMAb

Recombinant Anti-Thimet Oligopeptidase antibody [EPR7651] (ab154173)

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Overview

  • Product name

    Anti-Thimet Oligopeptidase antibody [EPR7651]
    See all Thimet Oligopeptidase primary antibodies

  • Description

    Rabbit monoclonal [EPR7651] to Thimet Oligopeptidase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Thimet Oligopeptidase aa 650 to the C-terminus. The exact sequence is proprietary.
    Database link: P52888

  • Positive control

    • Human cerebellum, fetal brain, K562, SH-SY5Y, THP1 and MCF7 lysates; MCF7 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 79 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.

    • Target

    Images

    • Western blot - Anti-Thimet Oligopeptidase antibody [EPR7651] (ab154173)
      Western blot - Anti-Thimet Oligopeptidase antibody [EPR7651] (ab154173)
      All lanes : Anti-Thimet Oligopeptidase antibody [EPR7651] (ab154173) at 1/1000 dilution

      Lane 1 : Human cerebellum lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : K562 cell lysate
      Lane 4 : SH-SY5Y cell lysate
      Lane 5 : THP1 cell lysate
      Lane 6 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 79 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Thimet Oligopeptidase antibody [EPR7651] (ab154173)
      Immunocytochemistry/ Immunofluorescence - Anti-Thimet Oligopeptidase antibody [EPR7651] (ab154173)
      Immunofluorescence analysis of MCF7 cells labeling Thimet Oligopeptidase with ab154173 at 1/100 dilution.

    References

    ab154173 has not yet been referenced specifically in any publications.

    Publishing research using ab154173? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question



    The antibodies ab59523 (Anti-NLN) and ab154173 (Anti-Thimet oligopeptidase) recognise two human peptidases of the M3 family that are rather similar at the sequence level (please find as attachment a protein sequence alignment).
    I was wondering if you could tell me (based on your knowledge of the epitopes) if there is possibility of cross reaction in either case?
    Also, if these particular antibodies would not be suitable to distinguish these two proteins, perhaps you could suggest others from your catalog.

    This issue is very important for my research and will likely determine whether or not we will purchase these antibodies.

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    Answer


    I have researched the immunogens and alignments for you:

    ab59523 Anti-NLN antibody : a blast of the RP3-Neurolysin antibody epitope shows about 59% identity with Thimet Oligopeptidase. It is immunogen affinity purified so there should be no or very little crossreactivity. This may also depend on the amount of TOP present in the sample.

    ab154173 Anti-Thimet Oligopeptidase antibody [EPR7651]: similarly, the immunogen has 53% alignment to the human NLN protein. This one is not affinity purified.

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