Recombinant
RabMAb

Recombinant Anti-Thiophosphate ester antibody [51-8] (ab133473)

Overview

  • Product name

    Anti-Thiophosphate ester antibody [51-8]
    See all Thiophosphate ester primary antibodies
  • Description

    Rabbit monoclonal [51-8] to Thiophosphate ester
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to Thiophosphate ester conjugated to keyhole limpet haemocyanin.

  • Positive control

    • HeLa cell lysate
  • General notes

    This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Kevan Shokat at University of California at San Francisco.

    This antibody is useful for studying kinase-substrate relationships of interest. The antibody is also context independent in that it can recognize serine, threonine, and tyrosine thiophosphorylated residues in a multitude of kinase phosphorylation consensus motifs.
    In order for this antibody to work in WB and IP, the customer needs to perform a specific kinase reaction using ATP gamma S and PNBM.
    See specific Protocol at the bottom of this page for details.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Primary antibody notes

    This antibody is useful for studying kinase-substrate relationships of interest. The antibody is also context independent in that it can recognize serine, threonine, and tyrosine thiophosphorylated residues in a multitude of kinase phosphorylation consensus motifs.
    In order for this antibody to work in WB and IP, the customer needs to perform a specific kinase reaction using ATP gamma S and PNBM.
    See specific Protocol at the bottom of this page for details.
  • Clonality

    Monoclonal
  • Clone number

    51-8
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/5000. Recommended: 0.5-1 ug of antibody per 1ug of kinase substrate

Target

  • Relevance

    Thiophosphate ester is used as fungicide.

Images

  • ab133473, at 1:5000, immunoblotting immunoprecipitated samples pre-resolved by SDS-PAGE. Myelin basic protein (MBP) was thiophosphorylated by AS GSK3 beta (GSK3 beta also catalyzes an auto thiophosporylation reaction). Samples were treated with or without p-nitrobenzyl mesylate (PNBM) for 1 hour at room temperature. PNBM was removed by size exclusion and 1mg/ml of HeLa cell lysate was added to samples.

    ab133473 was pre-incubated with protein G beads and was added to sample for an immunoprecipitation at 4°C overnight:

    (1) Thiophosphorylated MBP
    (2) PNBM treated thiophosphorylated MBP sample
    (3) HeLa cell lysate
    (4) HeLa cell lysate with PNBM treated thiophosphorylated MBP sample
    (5) Control

References

This product has been referenced in:

  • Shehata SN  et al. Identification of novel PCTAIRE-1/CDK16 substrates using a chemical genetic screen. Cell Signal 59:53-61 (2019). Read more (PubMed: 30880224) »
  • Callender TL  et al. Mek1 Down Regulates Rad51 Activity during Yeast Meiosis by Phosphorylation of Hed1. PLoS Genet 12:e1006226 (2016). WB . Read more (PubMed: 27483004) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T)
Total protein in input
0.1 µg
Immuno-precipitation step
Protein G
Specification
293T

Xiaoming Dai

Verified customer

Submitted Sep 23 2015

Answer


The main difference between ab92570 and ab133473 is the composition of the storage buffer.
The ab133473 is ideal for IP because it is supplied as cell culture supernatant with 0.01% sodium azide and the IgG concentration is at least 1 mg/ml, which is higher than ab92570.
Ab92570 can be used for IP, but it will require more antibody per reaction. This is why we launched the IP version (ab133473), which has higher concentration and no BSA in the buffer.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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