Product nameAnti-Thioredoxin / TRX antibody [3A1]
See all Thioredoxin / TRX primary antibodies
DescriptionMouse monoclonal [3A1] to Thioredoxin / TRX
Tested applicationsSuitable for: Flow Cyt, ELISA, WB, IPmore details
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Recombinant full length protein (Human) purified from E.coli
- HeLa cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.03% Sodium azide
Constituents: PBS, HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA
Concentration information loading...
Purification notesPurified from ascites.
Light chain typekappa
Our Abpromise guarantee covers the use of ab16965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|ELISA||Use at an assay dependent concentration.|
|WB||1/2000. Detects a band of approximately 12 kDa.|
|IP||Use at an assay dependent concentration.|
FunctionParticipates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
Sequence similaritiesBelongs to the thioredoxin family.
Contains 1 thioredoxin domain.
modificationsIn the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
Cellular localizationNucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
- Information by UniProt
- ADF antibody
- ATL derived factor antibody
- ATL-derived factor antibody
All lanes : Anti-Thioredoxin / TRX antibody [3A1] (ab16965) at 1/2000 dilution
All lanes : Cell lysates prepared from Hela cells
Lysates/proteins at 20 µg per lane.
ab16965 (1/2000) detecting Thioredoxin 1 by Western blot
Lane 1: HeLa cell lysate (20
Lane 2: Jurkat cell lysate (20
Secondary antibody conjugated to HRP
Overlay histogram showing HeLa cells stained with ab16965 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16965, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This product has been referenced in:
- Han Y et al. Reactive oxygen species promote tubular injury in diabetic nephropathy: The role of the mitochondrial ros-txnip-nlrp3 biological axis. Redox Biol 16:32-46 (2018). Read more (PubMed: 29475133) »
- Peeters PM et al. Silica-induced NLRP3 inflammasome activation in vitro and in rat lungs. Part Fibre Toxicol 11:58 (2014). WB ; Human . Read more (PubMed: 25406505) »