Recombinant
RabMAb

Recombinant Anti-Thioredoxin / TRX antibody [EPR6110] (ab133524)

Overview

  • Product name

    Anti-Thioredoxin / TRX antibody [EPR6110]
    See all Thioredoxin / TRX primary antibodies
  • Description

    Rabbit monoclonal [EPR6110] to Thioredoxin / TRX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in the Human Thioredoxin/TRX protein (UniProt ID: P10599).

  • Positive control

    • WB: MCF7, THP1, HeLa, and HepG2 whole cell lysate (ab7900) IHC-P: Human colon tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
IP 1/10 - 1/100.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
      ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
    • Sequence similarities

      Belongs to the thioredoxin family.
      Contains 1 thioredoxin domain.
    • Post-translational
      modifications

      In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
      In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
    • Cellular localization

      Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • ADF antibody
      • ATL derived factor antibody
      • ATL-derived factor antibody
      • DKFZp686B1993 antibody
      • MGC61975 antibody
      • SASP antibody
      • Surface associated sulphydryl protein antibody
      • Surface-associated sulphydryl protein antibody
      • testicular tissue protein Li 199 antibody
      • THIO_HUMAN antibody
      • Thioredoxin antibody
      • thioredoxin delta 3 antibody
      • TRDX antibody
      • TRX 1 antibody
      • Trx antibody
      • TRX1 antibody
      • TXN antibody
      • TXN delta 3 antibody
      • TXN protein antibody
      • zgc:92903 antibody
      see all

    Images

    • All lanes : Anti-Thioredoxin / TRX antibody [EPR6110] (ab133524) at 1/10000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : THP1 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 12 kDa
      Observed band size: 12 kDa

    • Immunohistochemistry analysis of Paraffin Embedded Human colon tissue labelling Thioredoxin/TRX with ab133524 at 1/250.
    • ab133524 (1/200) staining TRX in asynchronous HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see abreviews.

      See Abreview

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Zuo Z  et al. Interplay between Trx-1 and S100P promotes colorectal cancer cell epithelial-mesenchymal transition by up-regulating S100A4 through AKT activation. J Cell Mol Med N/A:N/A (2018). WB ; Human . Read more (PubMed: 29383839) »
    • Ren X  et al. Up-Regulation Thioredoxin Inhibits Advanced Glycation End Products-Induced Neurodegeneration. Cell Physiol Biochem 50:1673-1686 (2018). Read more (PubMed: 30384364) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Methanol
    Permeabilization
    No

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Sep 04 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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