Product nameAnti-Thioredoxin / TRX antibody [EPR6110]
See all Thioredoxin / TRX primary antibodies
DescriptionRabbit monoclonal [EPR6110] to Thioredoxin / TRX
Tested applicationsSuitable for: WB, IHC-P, ICC, IP, ICC/IFmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues in the Human Thioredoxin/TRX protein (UniProt ID: P10599).
- WB: MCF7, THP1, HeLa, and HepG2 whole cell lysate (ab7900) IHC-P: Human colon tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.12 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab133524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||1/100 - 1/250.|
|IP||1/10 - 1/100.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionParticipates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
Sequence similaritiesBelongs to the thioredoxin family.
Contains 1 thioredoxin domain.
modificationsIn the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
Cellular localizationNucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
- Information by UniProt
- ADF antibody
- ATL derived factor antibody
- ATL-derived factor antibody
All lanes : Anti-Thioredoxin / TRX antibody [EPR6110] (ab133524) at 1/10000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : THP1 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa
Immunohistochemistry analysis of Paraffin Embedded Human colon tissue labelling Thioredoxin/TRX with ab133524 at 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab133524 (1/200) staining TRX in asynchronous HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see abreviews.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Zuo Z et al. Interplay between Trx-1 and S100P promotes colorectal cancer cell epithelial-mesenchymal transition by up-regulating S100A4 through AKT activation. J Cell Mol Med N/A:N/A (2018). WB ; Human . Read more (PubMed: 29383839) »
- Ren X et al. Up-Regulation Thioredoxin Inhibits Advanced Glycation End Products-Induced Neurodegeneration. Cell Physiol Biochem 50:1673-1686 (2018). Read more (PubMed: 30384364) »