Recombinant
RabMAb

Recombinant Anti-Thioredoxin / TRX antibody [EPR6110] - BSA and Azide free (ab240066)

Overview

  • Product name

    Anti-Thioredoxin / TRX antibody [EPR6110] - BSA and Azide free
    See all Thioredoxin / TRX primary antibodies
  • Description

    Rabbit monoclonal [EPR6110] to Thioredoxin / TRX - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, ICC/IF, IP, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Thioredoxin/ TRX. The exact sequence is proprietary.

  • General notes

    ab240066 is the carrier-free version of ab133524 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240066 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
      ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
    • Sequence similarities

      Belongs to the thioredoxin family.
      Contains 1 thioredoxin domain.
    • Post-translational
      modifications

      In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
      In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
    • Cellular localization

      Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • ADF antibody
      • ATL derived factor antibody
      • ATL-derived factor antibody
      • DKFZp686B1993 antibody
      • MGC61975 antibody
      • SASP antibody
      • Surface associated sulphydryl protein antibody
      • Surface-associated sulphydryl protein antibody
      • testicular tissue protein Li 199 antibody
      • THIO_HUMAN antibody
      • Thioredoxin antibody
      • thioredoxin delta 3 antibody
      • TRDX antibody
      • TRX 1 antibody
      • Trx antibody
      • TRX1 antibody
      • TXN antibody
      • TXN delta 3 antibody
      • TXN protein antibody
      • zgc:92903 antibody
      see all

    Images

    • Immunohistochemistry analysis of Paraffin Embedded Human colon tissue labelling Thioredoxin/TRX with ab133524 at 1/250.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133524).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab133524 (1/200) staining TRX in asynchronous HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see abreviews.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133524).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133524).

    References

    ab240066 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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