Recombinant Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385)


  • Product name

    Anti-Thioredoxin / TRX antibody [EPR6111]
    See all Thioredoxin / TRX primary antibodies
  • Description

    Rabbit monoclonal [EPR6111] to Thioredoxin / TRX
  • Host species

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Thioredoxin/ TRX aa 50-150 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • MCF-7, THP-1, HeLa and HepG2 cell lysates
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab109385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 12 kDa.
Flow Cyt 1/100 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
      ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
    • Sequence similarities

      Belongs to the thioredoxin family.
      Contains 1 thioredoxin domain.
    • Post-translational

      In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
      In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
    • Cellular localization

      Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • ADF antibody
      • ATL derived factor antibody
      • ATL-derived factor antibody
      • DKFZp686B1993 antibody
      • MGC61975 antibody
      • SASP antibody
      • Surface associated sulphydryl protein antibody
      • Surface-associated sulphydryl protein antibody
      • testicular tissue protein Li 199 antibody
      • THIO_HUMAN antibody
      • Thioredoxin antibody
      • thioredoxin delta 3 antibody
      • TRDX antibody
      • TRX 1 antibody
      • Trx antibody
      • TRX1 antibody
      • TXN antibody
      • TXN delta 3 antibody
      • TXN protein antibody
      • zgc:92903 antibody
      see all


    • All lanes : Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385) at 1/10000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : THP-1 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 12 kDa

    • Overlay histogram showing HeLa cells stained with ab109385 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109385, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    ab109385 has not yet been referenced specifically in any publications.

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