Overview

  • Product name

    Anti-Thioredoxin 1 antibody [2C9]
  • Description

    Mouse monoclonal [2C9] to Thioredoxin 1
  • Host species

    Mouse
  • Specificity

    ab139677 recognizes E. coli Thioredoxin / TRX and TRX-tag-f Thioredoxin.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Escherichia coli
  • Immunogen

    Full length protein corresponding to Escherichia coli Thioredoxin 1.
    Database link: P0AA25

Properties

Applications

Our Abpromise guarantee covers the use of ab139677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 12 kDa.

Target

  • Relevance

    Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/ dithiol group within the conserved Cys-Gly-Pro-Cys active site. Specific protein disulfide targets for reduction by Trx include protein disulfide –isomerase (PDI) and a number of transcription factors such as p53, NFkB and AP-1. Trx is also apable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.
  • Cellular localization

    Cytoplasmic
  • Database links

    • Alternative names

      • ADF antibody
      • ATL-derived factor antibody
      • SASP antibody
      • Surface associated sulphydryl protein antibody
      • TRN antibody
      • TXN 1 antibody
      • TXN antibody
      see all

    Images

    • All lanes : Anti-Thioredoxin 1 antibody [2C9] (ab139677) at 1 µg/ml

      Lane 1 : Thioredoxin fusion protein expressed cell lysate; no induction
      Lane 2 : Thioredoxin fusion protein expressed cell lysate; induction for 30 min
      Lane 3 : Thioredoxin fusion protein expressed cell lysate; induction for 60 min
      Lane 4 : Thioredoxin fusion protein expressed cell lysate; induction for 90 min
      Lane 5 : Thioredoxin fusion protein expressed cell lysate; induction for 120 min
      Lane 6 : Thioredoxin fusion protein expressed cell lysate; induction for 150 min
      Lane 7 : Thioredoxin fusion protein expressed cell lysate; induction for 180 min
      Lane 8 : Thioredoxin fusion protein expressed cell lysate; induction for 210 min

      Developed using the ECL technique.

      Predicted band size: 12 kDa

    References

    This product has been referenced in:

    • Mohan J  et al. Cavin3 interacts with cavin1 and caveolin1 to increase surface dynamics of caveolae. J Cell Sci 128:979-91 (2015). Read more (PubMed: 25588833) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for your reply.

    We do not have the anti-Thioredoxin / TRX antibody [2C9] (ab139677) directly in stock, we would therefore expect the antibody to reach you within 2 weeks of when the order is placed.

    Can I just confirm that you will be placing the order yourself, or would you like for me to process it. If you would prefer for me to arrange it I would need your delivery and invoice address as well as your VAT number if you are claiming VAT free status.

    Please do let me know if I can be of any further assistance in this matter.

    Read More

    Answer

    Thank you for your reply.

    The antibody which I suggested to you, the mouse monoclonal anti-Thioredoxin / TRX antibody [2C9] (ab139677) is currently supplied in a 50 µg at a price of €370.00.

    I can however offer you a testing discount if you would be interested. This offer means that if you purchase ab139677 as normal, test the antibody with yourE. colisamples in an ELISA and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of ab139677 off any product). More information on this offer can be found here:

    www.abcam.com/collaborationdiscount

    Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

    If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab139677.

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

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