RecombinantRabMAb

Recombinant Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

Submit a review Submit a question References (15)
Western blot - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
  • Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
  • Flow Cytometry (Intracellular) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
  • Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15225] to Thioredoxin 2
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Thioredoxin 2 antibody [EPR15225]
    See all Thioredoxin 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR15225] to Thioredoxin 2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Raji and HeLa cell lysate. Human liver and mouse kidney tissue. HeLa and K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100 - 1/250.
WB
1/10000 - 1/50000. Detects a band of approximately 12 kDa (predicted molecular weight: 18 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100 - 1/250.
WB
1/10000 - 1/50000. Detects a band of approximately 12 kDa (predicted molecular weight: 18 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance

    Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide isomerase(PDI) and a number of transcription factors such as p53, NF-kB and AP-1. Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.

  • Cellular localization

    Mitochondrial

  • Database links

    • Alternative names

      • mitochondrial thioredoxin antibody
      • MT-TRX antibody
      • MTRX antibody
      • thioredoxin 2 precursor antibody
      • Thioredoxin mitochondrial antibody
      • Thioredoxin2 antibody
      • TRX 2 antibody
      • TRX2 antibody
      • TXN 2 antibody
      • TXN2 antibody
      see all

    Images

    • Western blot - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
      Western blot - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
      All lanes : Anti-Thioredoxin 2 antibody [EPR15225] (ab185544) at 1/10000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 18 kDa
      Observed band size: 12 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Thioredoxin 2 with ab185544 at 1/500 dilution. Counter stain Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

      Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Thioredoxin 2 with ab185544 at 1/500 dilution. Counter stain Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
      Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

      Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling Thioredoxin 2 with ab185544 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.

    • Flow Cytometry (Intracellular) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
      Flow Cytometry (Intracellular) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

      Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed K562 cells labeling Thioredoxin 2 with ab185544 at 1/150 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution. 

       

       

    • Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
      Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

    References (15)

    Publishing research using ab185544? Please let us know so that we can cite the reference in this datasheet.

    ab185544 has been referenced in 15 publications.

    • Li G  et al. Dysregulation of ferroptosis may involve in the development of non-small-cell lung cancer in Xuanwei area. J Cell Mol Med 25:2872-2884 (2021). PubMed: 33528895
    • Xiang C  et al. Yixin-Shu Capsules Ameliorated Ischemia-Induced Heart Failure by Restoring Trx2 and Inhibiting JNK/p38 Activation. Oxid Med Cell Longev 2021:8049079 (2021). PubMed: 33643519
    • Fan J  et al. ?-opioid receptor agonist U50488H attenuates postoperative cognitive dysfunction of cardiopulmonary bypass rats through the PI3K/AKT/Nrf2/HO-1 pathway. Mol Med Rep 23:N/A (2021). PubMed: 33649775
    • Quenneville S  et al. Hypoglycemia-Sensing Neurons of the Ventromedial Hypothalamus Require AMPK-Induced Txn2 Expression but Are Dispensable for Physiological Counterregulation. Diabetes 69:2253-2266 (2020). PubMed: 32839348
    • Bouitbir J  et al. Imatinib and Dasatinib Provoke Mitochondrial Dysfunction Leading to Oxidative Stress in C2C12 Myotubes and Human RD Cells. Front Pharmacol 11:1106 (2020). PubMed: 32792947
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab185544.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com