RecombinantRabMAb

Recombinant Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

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Western blot - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
  • Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
  • Flow Cytometry - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
  • Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15225] to Thioredoxin 2
  • Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Thioredoxin 2 antibody [EPR15225]
    See all Thioredoxin 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR15225] to Thioredoxin 2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Thioredoxin 2 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: Q99757

  • Positive control

    • Raji and HeLa cell lysate. Human liver and mouse kidney tissue. HeLa and K562 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab185544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/100 - 1/250.
WB 1/10000 - 1/50000. Detects a band of approximately 12 kDa (predicted molecular weight: 18 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance

    Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide isomerase(PDI) and a number of transcription factors such as p53, NF-kB and AP-1. Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.

  • Cellular localization

    Mitochondrial

  • Database links

    see all

  • Alternative names

    • mitochondrial thioredoxin antibody
    • MT-TRX antibody
    • MTRX antibody
    • thioredoxin 2 precursor antibody
    • Thioredoxin mitochondrial antibody
    • Thioredoxin2 antibody
    • TRX 2 antibody
    • TRX2 antibody
    • TXN 2 antibody
    • TXN2 antibody
    see all

Images

  • Western blot - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
    Western blot - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
    All lanes : Anti-Thioredoxin 2 antibody [EPR15225] (ab185544) at 1/10000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 18 kDa
    Observed band size: 12 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Thioredoxin 2 with ab185544 at 1/500 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Thioredoxin 2 with ab185544 at 1/500 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
    Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling Thioredoxin 2 with ab185544 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.

  • Flow Cytometry - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
    Flow Cytometry - Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

    Flow cytometry analysis of 2% paraformaldehyde fixed K562 cells labeling Thioredoxin 2 with ab185544 at 1/150 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

  • Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
    Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)

References (9)

Publishing research using ab185544? Please let us know so that we can cite the reference in this datasheet.

ab185544 has been referenced in 9 publications.

  • Gao J  et al. Metformin protects against PM2.5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase a2. Redox Biol 28:101345 (2020). PubMed: 31669973
  • Dang C  et al. Up-regulation of PGC-1a in neurons protects against experimental autoimmune encephalomyelitis. FASEB J 33:14811-14824 (2019). PubMed: 31718280
  • Chen C  et al. A Unique SUMO-Interacting Motif of Trx2 Is Critical for Its Mitochondrial Presequence Processing and Anti-oxidant Activity. Front Physiol 10:1089 (2019). PubMed: 31555141
  • Yuan F  et al. Mitochondrial targeted peptides preserve mitochondrial organization and decrease reversible myocardial changes in early swine metabolic syndrome. Cardiovasc Res 114:431-442 (2018). PubMed: 29267873
  • Chen X  et al. Inhalation of Hydrogen of Different Concentrations Ameliorates Spinal Cord Injury in Mice by Protecting Spinal Cord Neurons from Apoptosis, Oxidative Injury and Mitochondrial Structure Damages. Cell Physiol Biochem 47:176-190 (2018). PubMed: 29763919
  • Li YY  et al. Thioredoxin-2 protects against oxygen-glucose deprivation/reperfusion injury by inhibiting autophagy and apoptosis in H9c2 cardiomyocytes. Am J Transl Res 9:1471-1482 (2017). PubMed: 28386372
  • Machitani M  et al. MicroRNA miR-27 Inhibits Adenovirus Infection by Suppressing the Expression of SNAP25 and TXN2. J Virol 91:N/A (2017). WB ; Human . PubMed: 28356525
  • Li Y  et al. Intramyocardial injection of thioredoxin 2-expressing lentivirus alleviates myocardial ischemia-reperfusion injury in rats. Am J Transl Res 9:4428-4439 (2017). PubMed: 29118905
  • Harb OA  et al. Thioredoxin-Interact ing-Pro t e in [TXNIP] and Transglutaminase 2 [TGM2] Expression in Meningiomas of Different Grades and the Role of Their Expression in Meningioma Recurrence and Prognosis Asian Pac J Cancer Prev 18:2299-2308 (2017). PubMed: 28843270

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