Recombinant Anti-Thioredoxin 2 antibody [EPR15225] - BSA and Azide free (ab240292)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15225] to Thioredoxin 2 - BSA and Azide free
- Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Thioredoxin 2 antibody [EPR15225] - BSA and Azide free
See all Thioredoxin 2 primary antibodies -
Description
Rabbit monoclonal [EPR15225] to Thioredoxin 2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab240292 is the carrier-free version of ab185544.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15225 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Thioredoxin 2 antibody [EPR15225] (ab185544)
- PE Anti-Thioredoxin 2 antibody [EPR15225] (ab306283)
- APC Anti-Thioredoxin 2 antibody [EPR15225] (ab306284)
- HRP Anti-Thioredoxin 2 antibody [EPR15225] (ab306285)
- Alexa Fluor® 488 Anti-Thioredoxin 2 antibody [EPR15225] (ab309807)
- Alexa Fluor® 647 Anti-Thioredoxin 2 antibody [EPR15225] (ab310176)
- Alexa Fluor® 594 Anti-Thioredoxin 2 antibody [EPR15225] (ab310615)
- Alexa Fluor® 555 Anti-Thioredoxin 2 antibody [EPR15225] (ab312143)
- Alexa Fluor® 568 Anti-Thioredoxin 2 antibody [EPR15225] (ab312629)
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Conjugation kits
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 12 kDa (predicted molecular weight: 18 kDa).
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 12 kDa (predicted molecular weight: 18 kDa). |
Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Relevance
Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide isomerase(PDI) and a number of transcription factors such as p53, NF-kB and AP-1. Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins. -
Cellular localization
Mitochondrial -
Database links
- Entrez Gene: 25828 Human
- Entrez Gene: 56551 Mouse
- Entrez Gene: 79462 Rat
- Omim: 609063 Human
- SwissProt: Q99757 Human
- SwissProt: P97493 Mouse
- SwissProt: P97615 Rat
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Alternative names
- mitochondrial thioredoxin antibody
- MT-TRX antibody
- MTRX antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Thioredoxin 2 with ab185544 at 1/500 dilution. Counter stain Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185544).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Thioredoxin 2 with ab185544 at 1/500 dilution. Counter stain Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185544).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling Thioredoxin 2 with ab185544 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185544).
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Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed K562 cells labeling Thioredoxin 2 with ab185544 at 1/150 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185544).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab240292 has not yet been referenced specifically in any publications.